MPHOSPH8

Gene Information

Official Symbol: MPHOSPH8
Official Name: M-phase phosphoprotein 8
Aliases and Previous Symbols: N/A
Entrez ID: 54737
UniProt: Q99549
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). {ECO:0000269|PubMed:20871592, ECO:0000269|PubMed:26022416}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
42°C R08 exp539	-2.62
MK-1775 0.32μM R02 exp75	-2.39
LB-100 2μM R05 exp210	-2.22
Cadmium 60μM R08 exp462	-2.17
Resminostat 0.5μM R07 exp393	-2.02
Erlotinib 10μM R05 exp216	-1.79
Ethanol 0.01 R05 exp234	-1.77
MLN-4924 0.2μM R00 exp21	1.7
Clomiphene 4.4μM R02 exp68	1.74
Mdivi-1 15μM R05 exp217	1.75
UMK57 1μM R01 exp59	1.96
UM131593 0.2μM R03 exp106	2.22
Artemisinin 50μM R08 exp450	2.26

Global Fraction of Cell Lines Where Essential: 0/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 3037
Expression level (log2 read counts): 6.32

Expression Distribution

Gene	Correlation
FAM208A	0.48
PPHLN1	0.456

Ank 2
Chromo
Ank

positive regulation of methylation-dependent chromatin silencing
regulation of methylation-dependent chromatin silencing
negative regulation of single stranded viral RNA replication via double stranded DNA intermediate
positive regulation of chromatin silencing
nuclear nucleosome
regulation of single stranded viral RNA replication via double stranded DNA intermediate
nuclear heterochromatin
regulation of DNA methylation
heterochromatin
regulation of chromatin silencing
negative regulation of viral genome replication
methylated histone binding
negative regulation of gene expression, epigenetic
negative regulation of viral life cycle
regulation of viral genome replication
negative regulation of viral process
positive regulation of chromatin organization
regulation of gene silencing
regulation of viral life cycle
positive regulation of chromosome organization
regulation of chromatin organization
regulation of viral process
negative regulation of multi-organism process
regulation of symbiosis, encompassing mutualism through parasitism
regulation of gene expression, epigenetic
regulation of chromosome organization
regulation of DNA metabolic process
chromatin binding
positive regulation of organelle organization
regulation of multi-organism process
nucleolus
negative regulation of transcription, DNA-templated
positive regulation of cellular component organization
negative regulation of nucleic acid-templated transcription
negative regulation of RNA biosynthetic process
regulation of organelle organization
negative regulation of RNA metabolic process
negative regulation of cellular macromolecule biosynthetic process
negative regulation of nucleobase-containing compound metabolic process
negative regulation of macromolecule biosynthetic process