NOL9

Entrez Summary: N/A
UniProt Summary: Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA. {ECO:0000269|PubMed:21063389}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Pimelic-diphenylamide-106 5μM R00 exp28	-3.28
Rotenone 2μM R00 exp33	-1.96
THZ531 0.11 to 0.125μM on day4 R07 exp410	1.7
Cycloheximide 0.2μM R00 exp15	1.71
Quinacrine 2.5μM R03 exp146	1.74
Sucrose 20000μM R06 exp298	1.76
I-BRD9 4μM R07 exp365	1.84
PNU96415E 10μM R03 exp145	1.89
Calcipotriol 5μM R08 exp464	1.96
TGF-beta1 44ng/ml R08 exp528	2
GSK343 3μM R03 exp124	2.01
THZ531 0.11 to 0.175μM on day4 R07 exp413	2.02
Nifuroxazide 5μM R03 exp102	2.22
Benzoate 10000μM R08 exp455	2.24
Cyclosporin-A 3μM R07 exp348	2.39

Global Fraction of Cell Lines Where Essential: 667/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	1/1
bile duct	27/28
blood	25/28
bone	20/26
breast	26/33
central nervous system	53/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	14/16
kidney	21/21
liver	15/20
lung	70/75
lymphocyte	14/16
ovary	21/26
pancreas	24/24
peripheral nervous system	15/16
plasma cell	12/15
prostate	1/1
skin	22/24
soft tissue	8/9
thyroid	2/2
upper aerodigestive	19/22
urinary tract	27/29
uterus	4/5

Expression Distribution

Clp1
MobB

polynucleotide 5-hydroxyl-kinase activity
cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
endonucleolytic cleavage involved in rRNA processing
maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
maturation of LSU-rRNA
maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
cleavage involved in rRNA processing
maturation of 5.8S rRNA
intermediate filament cytoskeleton
ribosomal large subunit biogenesis
RNA phosphodiester bond hydrolysis, endonucleolytic
RNA phosphodiester bond hydrolysis
rRNA processing
rRNA metabolic process
nucleic acid phosphodiester bond hydrolysis
ribosome biogenesis
ncRNA processing
ribonucleoprotein complex biogenesis
ncRNA metabolic process
nucleolus
RNA processing
phosphorylation
RNA binding
ATP binding
RNA metabolic process
membrane
gene expression