TRIM33

Entrez Summary: The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008].
UniProt Summary: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade). {ECO:0000250, ECO:0000269|PubMed:10022127, ECO:0000269|PubMed:15820681, ECO:0000269|PubMed:16751102, ECO:0000269|PubMed:19135894}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Dexamethasone 0.006μM R07 exp351	-2.45
Dexamethasone 0.006 to 0.015μM on day4 R07 exp352	-2.43
Dimethyloxaloylglycine 11μM R07 exp314	-2.34
Rapamycin 1μM R00 exp29	-2.2
Rapamycin 10μM R00 exp30	-2.08
Rapamycin 2μM R07 exp306	-1.84
Senexin-A 25μM R07 exp400	-1.74
Olaparib 4μM R08 exp512	1.75
Thimerosal 0.85μM R08 exp529	1.82
Taxol 0.004μM R03 exp103	1.91
BML-284 0.09μM R08 exp460	2.36
UM131593 0.2μM R03 exp106	2.91
JQ1 0.8μM R08 exp435	3.01

Global Fraction of Cell Lines Where Essential: 0/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

co-SMAD binding
R-SMAD binding
negative regulation of BMP signaling pathway
regulation of BMP signaling pathway
regulation of transforming growth factor beta receptor signaling pathway
negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway
regulation of cellular response to transforming growth factor beta stimulus
negative regulation of cellular response to growth factor stimulus
regulation of transmembrane receptor protein serine/threonine kinase signaling pathway
ubiquitin-protein transferase activity
regulation of cellular response to growth factor stimulus
protein ubiquitination
protein modification by small protein conjugation
zinc ion binding
negative regulation of transcription by RNA polymerase II
protein modification by small protein conjugation or removal
negative regulation of transcription, DNA-templated
negative regulation of nucleic acid-templated transcription
negative regulation of RNA biosynthetic process
negative regulation of signal transduction
negative regulation of RNA metabolic process
negative regulation of cell communication
negative regulation of signaling
negative regulation of cellular macromolecule biosynthetic process
negative regulation of nucleobase-containing compound metabolic process
DNA binding
negative regulation of macromolecule biosynthetic process
negative regulation of cellular biosynthetic process
negative regulation of biosynthetic process
negative regulation of response to stimulus
negative regulation of gene expression