SF3B3

Gene Information

Official Symbol: SF3B3
Official Name: splicing factor 3b subunit 3
Aliases and Previous Symbols: N/A
Entrez ID: 23450
UniProt: Q15393
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008].
UniProt Summary: Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077). {ECO:0000269|PubMed:10882114, ECO:0000269|PubMed:12234937, ECO:0000269|PubMed:15146077, ECO:0000269|PubMed:27720643}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Hippuristanol 0.12μM R08 exp489 no dilution day6	-3.18
Rapamycin 0.001μM R08 exp429	-2.24
DABN 20μM R00 exp17	-1.85
Hippuristanol 0.12μM R08 exp488	-1.85
CI-1040 9.5μM R08 exp472	-1.83
BS-181 20μM R08 exp461	1.75
SGC2043 10μM R03 exp152	1.76
IL-3 9ng/ml R08 exp493	1.77
Momordin-Ic 10μM R08 exp506	1.78
CCCP 0.1μM R00 exp10	1.8
UM0011500 5μM R05 exp245	1.84
All-trans-Retinoic-Acid 40μM R07 exp334	1.89
Genistein 15μM R07 exp360	1.92
L-BMAA 500 to 750μM on day4 R04 exp185	2.13
Cholesterol 0.003 to 0.006 to 0.1μM on day2 then day6 R08 exp471	2.27
BAY-598 4μM R05 exp220	2.37
Parthenolide 2.5μM R05 exp238	2.38
Rapamycin 10μM R00 exp30	2.39
Golgicide-A 4μM R03 exp122	2.45
MLN-4924 0.2μM R00 exp21	2.65

Gene	Correlation
VASN	0.463
C14orf93	0.447
RPS2	0.434
PRIM1	0.433
AHCTF1	0.43
PAN3	0.426
ZNF513	0.426
PSMB4	0.422
PSMD3	0.421
PAN2	0.421
NPIPB5	0.417
NUP155	0.411
MTCH2	0.41
KIF3C	0.402

Global Fraction of Cell Lines Where Essential: 734/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	26/26
breast	32/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	20/21
liver	20/20
lung	74/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	28/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 79
Expression level (log2 read counts): 8.67

Expression Distribution

CPSF A
MMS1 N

U12-type spliceosomal complex
U2-type precatalytic spliceosome
catalytic step 2 spliceosome
negative regulation of protein catabolic process
nucleic acid binding
protein-containing complex binding
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile
mRNA splicing, via spliceosome
RNA splicing, via transesterification reactions
negative regulation of catabolic process
regulation of protein catabolic process
RNA splicing
mRNA processing
mRNA metabolic process
nucleolus
RNA processing
regulation of catabolic process
negative regulation of protein metabolic process
RNA metabolic process
gene expression