ZNF207

Entrez Summary: N/A
UniProt Summary: Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed:24462186, PubMed:24462187, PubMed:26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed:24462186, PubMed:24462187). Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187). {ECO:0000269|PubMed:24462186, ECO:0000269|PubMed:24462187, ECO:0000269|PubMed:26388440}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
CB-5083 0.4μM R08 exp468	1.7
D-Fructose 10000μM R06 exp279	1.73
Latrunculin-B 10μM R07 exp374	1.77
THZ531 0.11 to 0.175μM on day4 R07 exp413	1.84
Bisphenol S 100μM R08 exp458	1.87
Lead acetate 2000μM R08 exp498 no dilution day6	2
CAY10603 0.55μM R08 exp467	2.02
Senexin-A 25μM R07 exp400	2.03
THZ531 0.11 to 0.125μM on day4 R07 exp410	2.16
Lead acetate 2000μM R08 exp497	2.17
THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412	2.19
Geldanamycin 0.015 to 0.025μM on day4 R07 exp316	2.23
Olaparib 4μM R08 exp512	2.24
THZ531 0.225μM R08 exp444	2.26
PFI-3 10μM R03 exp144	2.38
Dynasore 10μM R04 exp180	2.42
Nutlin-3A 1.6μM R06 exp294	2.63
Ethanol 0.01 R05 exp234	3.44

Global Fraction of Cell Lines Where Essential: 339/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	7/28
blood	19/28
bone	9/26
breast	17/33
central nervous system	22/56
cervix	1/4
colorectal	8/17
esophagus	5/13
fibroblast	0/1
gastric	6/16
kidney	15/21
liver	6/20
lung	35/75
lymphocyte	6/16
ovary	13/26
pancreas	6/24
peripheral nervous system	11/16
plasma cell	13/15
prostate	0/1
skin	16/24
soft tissue	3/9
thyroid	1/2
upper aerodigestive	9/22
urinary tract	17/29
uterus	5/5

Expression Distribution

spindle matrix
attachment of spindle microtubules to kinetochore
mitotic spindle assembly checkpoint
spindle checkpoint
mitotic spindle checkpoint
spindle assembly checkpoint
negative regulation of mitotic metaphase/anaphase transition
negative regulation of metaphase/anaphase transition of cell cycle
negative regulation of mitotic sister chromatid separation
negative regulation of chromosome separation
negative regulation of mitotic sister chromatid segregation
microtubule polymerization
negative regulation of sister chromatid segregation
negative regulation of chromosome segregation
mitotic spindle assembly
negative regulation of mitotic nuclear division
negative regulation of nuclear division
microtubule polymerization or depolymerization
regulation of mitotic metaphase/anaphase transition
regulation of metaphase/anaphase transition of cell cycle
regulation of mitotic sister chromatid separation
regulation of chromosome separation
regulation of mitotic sister chromatid segregation
mitotic spindle organization
regulation of sister chromatid segregation
kinetochore
protein polymerization
condensed chromosome kinetochore
microtubule bundle formation
spindle assembly
regulation of chromosome segregation
microtubule cytoskeleton organization involved in mitosis
mitotic sister chromatid segregation
spindle
negative regulation of chromosome organization
sister chromatid segregation
mitotic nuclear division
mitotic cell cycle checkpoint
spindle organization
regulation of mitotic nuclear division