HSF1

Gene Information

Official Symbol: HSF1
Official Name: heat shock transcription factor 1
Aliases and Previous Symbols: N/A
Entrez ID: 3297
UniProt: Q00613
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed:1871105, PubMed:11447121, PubMed:1986252, PubMed:7760831, PubMed:7623826, PubMed:8946918, PubMed:8940068, PubMed:9341107, PubMed:9121459, PubMed:9727490, PubMed:9499401, PubMed:9535852, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:25963659, PubMed:26754925). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:9727490, PubMed:11583998, PubMed:16278218). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:1871105, PubMed:1986252, PubMed:8455624, PubMed:7935471, PubMed:7623826, PubMed:8940068, PubMed:9727490, PubMed:9499401, PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:25963659, PubMed:26754925). Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed:11583998, PubMed:16278218). Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed:1986252, PubMed:26727489). Binds to chromatin at heat shock gene promoters (PubMed:25963659). Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed:9341107). Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner (PubMed:14707147). Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed:17897941). Plays a role in the regulation of mitotic progression (PubMed:18794143). Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage- dependent manner (PubMed:26359349). Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed:26754925). {ECO:0000269|PubMed:10359787, ECO:0000269|PubMed:11447121, ECO:0000269|PubMed:11583998, ECO:0000269|PubMed:12659875, ECO:0000269|PubMed:12917326, ECO:0000269|PubMed:14707147, ECO:0000269|PubMed:15016915, ECO:0000269|PubMed:16278218, ECO:0000269|PubMed:17897941, ECO:0000269|PubMed:1871105, ECO:0000269|PubMed:18794143, ECO:0000269|PubMed:1986252, ECO:0000269|PubMed:25963659, ECO:0000269|PubMed:26359349, ECO:0000269|PubMed:26727489, ECO:0000269|PubMed:26754925, ECO:0000269|PubMed:7623826, ECO:0000269|PubMed:7760831, ECO:0000269|PubMed:7935471, ECO:0000269|PubMed:8455624, ECO:0000269|PubMed:8940068, ECO:0000269|PubMed:8946918, ECO:0000269|PubMed:9121459, ECO:0000269|PubMed:9341107, ECO:0000269|PubMed:9499401, ECO:0000269|PubMed:9535852, ECO:0000269|PubMed:9727490}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Geldanamycin 0.015 to 0.025μM on day4 R07 exp316	-2.13
Radicicol 0.16μM R05 exp242	-1.95
Tanespimycin 14μM R08 exp527	-1.92
PU-H71 1μM R08 exp515	-1.91
Geldanamycin 0.015 to 0.05μM on day4 R07 exp317	-1.75
Beta-Estradiol 0.55μM R07 exp425	1.71
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	1.71
Genistein 15μM R07 exp360	1.73
UM0131023 0.05μM R05 exp248	1.78
CCCP 0.1μM R00 exp10	1.81
GSK-J4 1μM R07 exp362	1.81
Ixabepilone 0.004 to 0.005μM on day4 R04 exp184	1.88
Adefovir 20μM R07 exp332	1.88
IU1-C 25μM R04 exp183	1.89
Taxol 0.004μM R03 exp103	1.91
ABT-702 5μM R07 exp318	1.96
Apcin 25μM plus proTAME 2μM R04 exp177	1.96
Nicotine 3000μM R08 exp510	1.98
Phorbol-12-myristate-13-acetate 0.57μM R08 exp514	2.01
FK-506 5μM R07 exp358	2.04
Mdivi-1 15μM R05 exp217	2.14
RO-3306 3 to 4μM on day4 R04 exp165	2.2
Nutlin-3A 1.6μM R06 exp294	3.87

Global Fraction of Cell Lines Where Essential: 49/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	4/28
blood	1/28
bone	0/26
breast	4/33
central nervous system	2/56
cervix	0/4
colorectal	1/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	2/21
liver	3/20
lung	4/75
lymphocyte	1/16
ovary	3/26
pancreas	0/24
peripheral nervous system	2/16
plasma cell	1/15
prostate	0/1
skin	1/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	1/22
urinary tract	2/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 1662
Expression level (log2 read counts): 6.21

Expression Distribution

Vert HS TF
HSF DNA-bind

cellular response to nitroglycerin
translation elongation factor binding
response to diamide
cellular response to diamide
response to nitroglycerin
sequence-specific single stranded DNA binding
cellular response to L-glutamine
response to L-glutamine
response to hypobaric hypoxia
response to psychosocial stress
positive regulation of inclusion body assembly
positive regulation of mRNA polyadenylation
positive regulation of apoptotic DNA fragmentation
cellular response to sodium arsenite
nuclear stress granule
positive regulation of DNA catabolic process
positive regulation of microtubule binding
pronucleus
embryonic process involved in female pregnancy
response to sodium arsenite
negative regulation of double-strand break repair via nonhomologous end joining
cellular response to salt
regulation of protein heterodimerization activity
euchromatin
positive regulation of transcription from RNA polymerase II promoter in response to heat stress
STAT family protein binding
cellular response to potassium ion
regulation of microtubule binding
regulation of apoptotic DNA fragmentation
positive regulation of mRNA 3-end processing
negative regulation of inclusion body assembly
response to herbicide
regulation of DNA catabolic process
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
response to potassium ion
positive regulation of execution phase of apoptosis
regulation of inclusion body assembly
response to insecticide
regulation of mRNA polyadenylation
cellular response to arsenic-containing substance