KARS
Gene Information
- Official Symbol: KARS1
- Official Name: lysyl-tRNA synthetase 1
- Aliases and Previous Symbols: N/A
- Entrez ID: 3735
- UniProt: Q15046
- Interactions: BioGRID
- PubMed articles: Open PubMed
- OMIM: Open OMIM
Function Summary
- Entrez Summary: N/A
- UniProt Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739). {ECO:0000269|PubMed:14975237, ECO:0000269|PubMed:15851690, ECO:0000269|PubMed:18029264, ECO:0000269|PubMed:19524539, ECO:0000269|PubMed:28887846, ECO:0000269|PubMed:5338216, ECO:0000269|PubMed:9278442}.
Pfam Domains GO Terms
Pfam Domains
| tRNA-synt 2 |
| tRNA anti |
GO Terms
| lysine-tRNA ligase activity |
| ATP adenylyltransferase activity |
| lysyl-tRNA aminoacylation |
| basophil activation involved in immune response |
| basophil activation |
| diadenosine tetraphosphate metabolic process |
| diadenosine polyphosphate biosynthetic process |
| diadenosine tetraphosphate biosynthetic process |
| positive regulation of metallopeptidase activity |
| diadenosine polyphosphate metabolic process |
| positive regulation of cytokine secretion involved in immune response |
| aminoacyl-tRNA synthetase multienzyme complex |
| regulation of metallopeptidase activity |
| positive regulation of macrophage chemotaxis |
| regulation of cytokine secretion involved in immune response |
| positive regulation of cytokine production involved in inflammatory response |
| amino acid binding |
| positive regulation of macrophage migration |
| positive regulation of macrophage activation |
| regulation of macrophage chemotaxis |
| positive regulation of p38MAPK cascade |
| ERK1 and ERK2 cascade |
| response to X-ray |
| regulation of macrophage migration |
| regulation of cytokine production involved in inflammatory response |
| regulation of p38MAPK cascade |
| regulation of granulocyte chemotaxis |
| tRNA aminoacylation for protein translation |
| regulation of mononuclear cell migration |
| tRNA aminoacylation |
| amino acid activation |
| regulation of macrophage activation |
| positive regulation of cytokine production involved in immune response |
| tRNA binding |
| regulation of cytokine production involved in immune response |
| positive regulation of leukocyte chemotaxis |
| positive regulation of production of molecular mediator of immune response |
| regulation of leukocyte chemotaxis |
| tumor necrosis factor-mediated signaling pathway |
| positive regulation of leukocyte migration |
| tRNA processing |
| positive regulation of chemotaxis |
| positive regulation of cytokine secretion |
| positive regulation of inflammatory response |
| regulation of production of molecular mediator of immune response |
| response to ionizing radiation |
| activation of MAPK activity |
| positive regulation of stress-activated MAPK cascade |
| positive regulation of stress-activated protein kinase signaling cascade |
| tRNA metabolic process |
| positive regulation of peptidase activity |
| regulation of leukocyte migration |
| regulation of cytokine secretion |
| positive regulation of ERK1 and ERK2 cascade |
| regulation of chemotaxis |
| positive regulation of immune effector process |
| regulation of stress-activated MAPK cascade |
| regulation of stress-activated protein kinase signaling cascade |
| cellular response to tumor necrosis factor |
| nucleotide biosynthetic process |
| nucleoside phosphate biosynthetic process |
| positive regulation of MAP kinase activity |
| response to tumor necrosis factor |
| positive regulation of protein secretion |
| positive regulation of peptide secretion |
| regulation of ERK1 and ERK2 cascade |
| cellular amino acid metabolic process |
| activation of protein kinase activity |
| regulation of inflammatory response |
| positive regulation of protein serine/threonine kinase activity |
| regulation of MAP kinase activity |
| positive regulation of proteolysis |
| mitochondrial matrix |
| MAPK cascade |
| translation |
| ncRNA processing |
| signal transduction by protein phosphorylation |
| positive regulation of secretion by cell |
| positive regulation of leukocyte activation |
| peptide biosynthetic process |
| positive regulation of cell activation |
| positive regulation of protein transport |
| positive regulation of secretion |
| response to radiation |
| regulation of peptidase activity |
| nucleotide metabolic process |
| positive regulation of cytokine production |
| nucleoside phosphate metabolic process |
| positive regulation of establishment of protein localization |
| regulation of protein secretion |
| regulation of immune effector process |
| positive regulation of defense response |
| ncRNA metabolic process |
| regulation of peptide secretion |
| positive regulation of cell migration |
| granulocyte activation |
| regulation of protein serine/threonine kinase activity |
| myeloid cell activation involved in immune response |
| positive regulation of cell motility |
| amide biosynthetic process |
| peptide metabolic process |
| positive regulation of protein kinase activity |
| organophosphate biosynthetic process |
| positive regulation of cellular component movement |
| nucleobase-containing small molecule metabolic process |
| positive regulation of MAPK cascade |
| positive regulation of locomotion |
| positive regulation of kinase activity |
| myeloid leukocyte activation |
| positive regulation of response to external stimulus |
| regulation of leukocyte activation |
| leukocyte activation involved in immune response |
| cell activation involved in immune response |
| regulation of cell activation |
| positive regulation of transferase activity |
| cytokine-mediated signaling pathway |
| negative regulation of cell population proliferation |
| regulation of cytokine production |
| viral process |
| regulation of protein transport |
| regulation of proteolysis |
| regulation of cellular response to stress |
| regulation of peptide transport |
| regulation of establishment of protein localization |
| regulation of secretion by cell |
| regulation of MAPK cascade |
| regulation of defense response |
| positive regulation of hydrolase activity |
| symbiotic process |
| cellular amide metabolic process |
| regulation of protein kinase activity |
| regulation of secretion |
| interspecies interaction between organisms |
| regulation of cell migration |
| positive regulation of immune response |
| regulation of kinase activity |
| RNA processing |
| protein homodimerization activity |
| organophosphate metabolic process |
| carboxylic acid metabolic process |
| regulation of cell motility |
| leukocyte activation |
| protein phosphorylation |
| regulation of transferase activity |
| regulation of locomotion |
| positive regulation of transport |
| regulation of cellular component movement |
| oxoacid metabolic process |
| cellular response to cytokine stimulus |
| positive regulation of protein phosphorylation |
| positive regulation of intracellular signal transduction |
| organic acid metabolic process |
| regulation of protein localization |
| positive regulation of phosphorylation |
| identical protein binding |
| cell activation |
| immune effector process |
| regulation of response to external stimulus |
| nucleobase-containing compound biosynthetic process |
| response to cytokine |
| positive regulation of phosphate metabolic process |
| positive regulation of phosphorus metabolic process |
| regulation of immune response |
| positive regulation of immune system process |
| response to abiotic stimulus |
| heterocycle biosynthetic process |
| aromatic compound biosynthetic process |
| positive regulation of protein modification process |
| mitochondrion |
| regulation of hydrolase activity |
| phosphorylation |
| organic cyclic compound biosynthetic process |
| organonitrogen compound biosynthetic process |
| positive regulation of catalytic activity |
| regulation of protein phosphorylation |
| regulation of response to stress |
| ATP binding |
| positive regulation of transcription, DNA-templated |
| regulation of phosphorylation |
| extracellular space |
| positive regulation of cellular protein metabolic process |
| regulation of cell population proliferation |
| cellular nitrogen compound biosynthetic process |
| positive regulation of nucleic acid-templated transcription |
| positive regulation of RNA biosynthetic process |
| positive regulation of signal transduction |
| regulation of immune system process |
| RNA metabolic process |
| intracellular signal transduction |
| positive regulation of protein metabolic process |
| cellular macromolecule biosynthetic process |
| positive regulation of RNA metabolic process |
| positive regulation of multicellular organismal process |
| small molecule metabolic process |
| macromolecule biosynthetic process |
| positive regulation of molecular function |
| regulation of phosphate metabolic process |
| regulation of phosphorus metabolic process |
| positive regulation of cell communication |
| positive regulation of signaling |
| regulation of intracellular signal transduction |
| regulation of protein modification process |
| regulation of transport |
| immune response |
| positive regulation of nucleobase-containing compound metabolic process |
| positive regulation of macromolecule biosynthetic process |
| positive regulation of cellular biosynthetic process |
| positive regulation of gene expression |
| gene expression |
| positive regulation of biosynthetic process |
CRISPR Data
Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Compound Hit
Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Global Fraction of Cell Lines Where Essential: 738/739
| Tissue | Fraction Of Cell Lines Where Essential |
|---|---|
| 1290807.0 | 1/1 |
| 909776.0 | 1/1 |
| bile duct | 28/28 |
| blood | 28/28 |
| bone | 26/26 |
| breast | 33/33 |
| central nervous system | 56/56 |
| cervix | 4/4 |
| colorectal | 17/17 |
| esophagus | 13/13 |
| fibroblast | 1/1 |
| gastric | 16/16 |
| kidney | 21/21 |
| liver | 20/20 |
| lung | 75/75 |
| lymphocyte | 16/16 |
| ovary | 26/26 |
| pancreas | 24/24 |
| peripheral nervous system | 16/16 |
| plasma cell | 15/15 |
| prostate | 1/1 |
| skin | 24/24 |
| soft tissue | 9/9 |
| thyroid | 2/2 |
| upper aerodigestive | 22/22 |
| urinary tract | 29/29 |
| uterus | 5/5 |
Essentiality in NALM6
- Essentiality Rank: 132
- Expression level (log2 read counts): 7.57
Expression Distribution
KARS Expression in NALM6 Cells: 7.57
