MTHFD1

Gene Information

Official Symbol: MTHFD1
Official Name: methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
Aliases and Previous Symbols: N/A
Entrez ID: 4522
UniProt: P11586
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008].
UniProt Summary: N/A

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Parthenolide 2.5μM R05 exp238	-2.23
BH1 1μM R04 exp169	-2.13
Menadione 5μM R06 exp292	-2.06
THZ531 0.11 to 0.125μM on day4 R07 exp410	-2.02
Bisphenol A 100μM R06 exp269	-1.98
Sucrose 20000μM R06 exp298	-1.81
BN82002 4μM R07 exp340	-1.8
All-trans-Retinoic-Acid 8μM R07 exp333	-1.77
Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53	-1.7
Dimethyl-Sulfoxide 1pc R03 exp120	1.71
FK-506 30μM R07 exp359	1.71
Golgicide-A 4μM R03 exp122	1.81
GSK343 3μM R03 exp124	1.85
PNU96415E 10μM R03 exp145	1.89
QNZ 0.01μM R05 exp241	1.89
Torin1 0.08μM R02 exp82	2.12
Dinaciclib 0.007μM R07 exp355	2.15
SGC0946 7μM R03 exp151	2.16
Phenformin 20μM R05 exp212	2.19
AICAR 240μM R05 exp211	3.19

Global Fraction of Cell Lines Where Essential: 3/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	1/16
plasma cell	1/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 182
Expression level (log2 read counts): 7.8

Expression Distribution

Gene	Correlation
GART	0.494
ATIC	0.473

THF DHG CYH C
THF DHG CYH
FTHFS

methylenetetrahydrofolate dehydrogenase [NAD(P)+] activity
histidine biosynthetic process
formate-tetrahydrofolate ligase activity
10-formyltetrahydrofolate biosynthetic process
methylenetetrahydrofolate dehydrogenase (NAD+) activity
methenyltetrahydrofolate cyclohydrolase activity
aromatic amino acid family biosynthetic process
methylenetetrahydrofolate dehydrogenase (NADP+) activity
10-formyltetrahydrofolate metabolic process
embryonic neurocranium morphogenesis
tetrahydrofolate biosynthetic process
embryonic viscerocranium morphogenesis
histidine metabolic process
folic acid-containing compound biosynthetic process
tetrahydrofolate interconversion
purine nucleobase biosynthetic process
imidazole-containing compound metabolic process
methionine biosynthetic process
dicarboxylic acid biosynthetic process
pteridine-containing compound biosynthetic process
methionine metabolic process
serine family amino acid biosynthetic process
sulfur amino acid biosynthetic process
tetrahydrofolate metabolic process
folic acid metabolic process
nucleobase biosynthetic process
purine nucleobase metabolic process
aspartate family amino acid biosynthetic process
one-carbon metabolic process
folic acid-containing compound metabolic process
pteridine-containing compound metabolic process
aromatic amino acid family metabolic process
sulfur amino acid metabolic process
serine family amino acid metabolic process
nucleobase metabolic process
embryonic cranial skeleton morphogenesis
cellular modified amino acid biosynthetic process
pigment biosynthetic process
aspartate family amino acid metabolic process
pigment metabolic process