NELFA

Gene Information

Official Symbol: NELFA
Official Name: negative elongation factor complex member A
Aliases and Previous Symbols: N/A
Entrez ID: 7469
UniProt: Q9H3P2
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008].
UniProt Summary: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and ssRNA in a sequence- and structure-dependent manner. Probably required to interact with the RNA polymerase II complex. {ECO:0000269|PubMed:10199401, ECO:0000269|PubMed:12563561, ECO:0000269|PubMed:12612062, ECO:0000305}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Diepoxybutane 3μM R07 exp354	-2.74
MS023 7μM R03 exp135	-2.46
Dorsomorphin 5μM R07 exp357	-2.32
Pimelic-diphenylamide-106 5μM R00 exp28	-2.3
Tozasertib 0.1μM R07 exp414	-2.03
Docetaxel 0.002μM R01 exp45	-2.02
HMS-I1 1μM R01 exp46	-2.02
42°C R08 exp539	-1.86
Hydroxyurea 100μM R07 exp329	-1.84
UMK57 1μM R01 exp59	-1.83
JQ1 0.8μM R08 exp435	-1.78
Etoposide 0.3μM R05 exp199	-1.74
HMS-I1 10μM R06 exp256	-1.74
Oligomycin-A 20μM R00 exp26	-1.72
Trimetrexate 0.03μM R08 exp535	2.5

Gene	Correlation
NELFB	0.508
NELFE	0.487
BRD2	0.481
NELFCD	0.475
NARFL	0.45
RPL8	0.433
PSMB4	0.428
PRIM1	0.425
CHMP4B	0.417
PDS5B	0.416
UTP3	0.412
PSMA6	0.412
DDX3X	0.411
IPPK	0.408

Global Fraction of Cell Lines Where Essential: 313/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	8/28
blood	10/28
bone	11/25
breast	17/33
central nervous system	29/56
cervix	1/4
colorectal	5/17
esophagus	5/13
fibroblast	0/1
gastric	5/15
kidney	8/21
liver	13/20
lung	28/75
lymphocyte	10/14
ovary	12/26
pancreas	9/24
peripheral nervous system	7/16
plasma cell	6/15
prostate	1/1
skin	10/24
soft tissue	3/7
thyroid	1/2
upper aerodigestive	11/22
urinary tract	11/29
uterus	2/5

Essentiality in NALM6

Essentiality Rank: 1561
Expression level (log2 read counts): 5.67

Expression Distribution

NELF complex
negative regulation of transcription elongation from RNA polymerase II promoter
negative regulation of DNA-templated transcription, elongation
regulation of transcription elongation from RNA polymerase II promoter
positive regulation of viral transcription
regulation of DNA-templated transcription, elongation
regulation of viral transcription
transcription elongation from RNA polymerase II promoter
DNA-templated transcription, elongation
positive regulation of viral process
regulation of viral process
regulation of symbiosis, encompassing mutualism through parasitism
nuclear body
transcription by RNA polymerase II
positive regulation of multi-organism process
transcription, DNA-templated
nucleic acid-templated transcription
RNA biosynthetic process
regulation of multi-organism process
negative regulation of transcription by RNA polymerase II
nucleobase-containing compound biosynthetic process
heterocycle biosynthetic process
aromatic compound biosynthetic process
negative regulation of transcription, DNA-templated
negative regulation of nucleic acid-templated transcription
negative regulation of RNA biosynthetic process
organic cyclic compound biosynthetic process
negative regulation of RNA metabolic process
negative regulation of cellular macromolecule biosynthetic process
negative regulation of nucleobase-containing compound metabolic process
negative regulation of macromolecule biosynthetic process
negative regulation of cellular biosynthetic process
negative regulation of biosynthetic process
cellular nitrogen compound biosynthetic process
RNA metabolic process
cellular macromolecule biosynthetic process
negative regulation of gene expression
macromolecule biosynthetic process
gene expression