PHF2

Entrez Summary: This gene encodes a protein which contains a zinc finger-like PHD (plant homeodomain) finger, distinct from other classes of zinc finger motifs, and a hydrophobic and highly conserved domain. The PHD finger shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. [provided by RefSeq, Jul 2008].
UniProt Summary: Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. {ECO:0000269|PubMed:20129925, ECO:0000269|PubMed:21167174, ECO:0000269|PubMed:21532585}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Geldanamycin 0.015 to 0.05μM on day4 R07 exp317	1.73
THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412	1.73
NMS-873 0.07μM R07 exp380	1.77
PFI-2 4μM R05 exp239	1.8
Geldanamycin 0.01μM R05 exp235	1.85
Pomalidomide 20μM R07 exp391	2.04
Geldanamycin 0.015 to 0.025μM on day4 R07 exp316	2.08
Dinaciclib 0.007μM R07 exp355	2.65

Global Fraction of Cell Lines Where Essential: 1/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	1/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

JmjC
PHD

negative regulation of chromatin silencing at rDNA
regulation of chromatin silencing at rDNA
histone demethylase activity (H3-K9 specific)
histone H3-K9 demethylation
histone demethylase activity
negative regulation of chromatin silencing
dioxygenase activity
histone lysine demethylation
histone demethylation
protein demethylation
protein dealkylation
negative regulation of gene silencing
regulation of chromatin silencing
methylated histone binding
negative regulation of chromatin organization
positive regulation of gene expression, epigenetic
demethylation
kinetochore
condensed chromosome kinetochore
liver development
regulation of gene silencing
hepaticobiliary system development
iron ion binding
negative regulation of chromosome organization
regulation of chromatin organization
regulation of gene expression, epigenetic
transcription coactivator activity
regulation of chromosome organization
histone modification
negative regulation of organelle organization
covalent chromatin modification
gland development
chromatin organization
negative regulation of cellular component organization
zinc ion binding
nucleolus
oxidation-reduction process
chromosome organization
regulation of organelle organization
positive regulation of transcription, DNA-templated