ROBO1

Gene Information

Official Symbol: ROBO1
Official Name: roundabout guidance receptor 1
Aliases and Previous Symbols: N/A
Entrez ID: 6091
UniProt: Q9Y6N7
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009].
UniProt Summary: Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10102268, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP- bound RHOA (PubMed:26529257). May be required for lung development (By similarity). {ECO:0000250|UniProtKB:O89026, ECO:0000269|PubMed:10102268, ECO:0000269|PubMed:24560577, ECO:0000269|PubMed:26529257, ECO:0000305}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
2-Deoxy-D-glucose 800μM R05 exp214	-1.87
3-Bromopyruvate 7μM R04 exp175	-1.86
Bafilomycin-A1 0.009μM R08 exp454	-1.83
Cholesterol 0.003 to 0.006 to 0.1μM on day2 then day6 R08 exp471	-1.74
THZ531 0.11 to 0.175μM on day4 R07 exp413	1.73
YM155 0.0002μM R01 exp61	1.93
Selumetinib 20μM R02 exp81	2.01
UM131593 0.1μM R01 exp58	2.06
A-196 10μM R03 exp114	2.17
Trientine 500μM R08 exp534	2.45

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 5086
Expression level (log2 read counts): 1.18

Expression Distribution

I-set
fn3
V-set
ig

chemorepulsion involved in postnatal olfactory bulb interneuron migration
negative regulation of negative chemotaxis
cerebral cortex tangential migration using cell-cell interactions
postnatal olfactory bulb interneuron migration
negative regulation of mammary gland epithelial cell proliferation
positive regulation of Notch signaling pathway involved in heart induction
regulation of Notch signaling pathway involved in heart induction
axon midline choice point recognition
regulation of negative chemotaxis
positive regulation of heart induction
Roundabout signaling pathway
substrate-dependent cerebral cortex tangential migration
LRR domain binding
positive regulation of vascular endothelial growth factor signaling pathway
axon choice point recognition
regulation of heart induction
negative regulation of chemokine-mediated signaling pathway
tangential migration from the subventricular zone to the olfactory bulb
olfactory bulb interneuron development
axon guidance receptor activity
cerebral cortex tangential migration
regulation of chemokine-mediated signaling pathway
olfactory bulb interneuron differentiation
regulation of mammary gland epithelial cell proliferation
regulation of vascular endothelial growth factor signaling pathway
pulmonary valve morphogenesis
regulation of cellular response to vascular endothelial growth factor stimulus
endocardial cushion formation
pulmonary valve development
cell migration involved in sprouting angiogenesis
substrate-dependent cell migration
outflow tract septum morphogenesis
aortic valve morphogenesis
positive regulation of Rho protein signal transduction
blood vessel endothelial cell migration
forebrain neuron development
olfactory bulb development
aortic valve development
olfactory lobe development
endocardial cushion morphogenesis