USP5

Gene Information

Official Symbol: USP5
Official Name: ubiquitin specific peptidase 5
Aliases and Previous Symbols: N/A
Entrez ID: 8078
UniProt: P45974
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. {ECO:0000269|PubMed:19098288}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Cytochalasin-B 5μM R07 exp349	-2.75
Etoposide 1μM R00 exp19	-2.74
AICAR 240μM R05 exp211	-2.63
Aminopterin 0.005μM R07 exp335	-2.49
2-Deoxy-D-glucose 800μM R05 exp214	-2.42
42°C R08 exp539	-2.01
5-Fluorouracil 20μM R00 exp2	-2
GSK461364A 0.1μM R03 exp126	-2
BI-D1870 3.15μM R02 exp66	-1.92
ML-792 0.2μM R08 exp505	-1.91
RO-3307 4.7μM R02 exp80	-1.82
6-Thio-2-deoxyguanosine 2μM R06 exp259	-1.81
Centrinone 0.5μM R07 exp343	-1.77
Milciclib 2μM R08 exp502	-1.77
Pimelic-diphenylamide-106 5μM R00 exp28	-1.71
Tozasertib 0.1μM R07 exp414	-1.71
KU-0063794 3.8μM R02 exp71	-1.7
ZLN024 50μM R08 exp538	1.72

Global Fraction of Cell Lines Where Essential: 717/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	27/28
bone	24/26
breast	33/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	12/13
fibroblast	1/1
gastric	16/16
kidney	20/21
liver	20/20
lung	72/75
lymphocyte	15/16
ovary	23/26
pancreas	22/24
peripheral nervous system	15/16
plasma cell	15/15
prostate	1/1
skin	23/24
soft tissue	8/9
thyroid	2/2
upper aerodigestive	21/22
urinary tract	29/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 184
Expression level (log2 read counts): 7.1

Expression Distribution

Gene	Correlation
NIPBL	0.425
CHMP4B	0.423
MTCH2	0.422
PSMA6	0.411
PSMC1	0.41

UCH
zf-UBP
UBA

protein K48-linked deubiquitination
thiol-dependent ubiquitinyl hydrolase activity
ubiquitin binding
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
cysteine-type endopeptidase activity
positive regulation of ubiquitin-dependent protein catabolic process
positive regulation of proteasomal protein catabolic process
thiol-dependent ubiquitin-specific protease activity
positive regulation of proteolysis involved in cellular protein catabolic process
regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of cellular protein catabolic process
regulation of ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
regulation of proteolysis involved in cellular protein catabolic process
positive regulation of protein catabolic process
regulation of cellular protein catabolic process
lysosome
protein deubiquitination
protein modification by small protein removal
positive regulation of proteolysis
positive regulation of cellular catabolic process
regulation of protein catabolic process
positive regulation of catabolic process
ubiquitin-dependent protein catabolic process
modification-dependent protein catabolic process
modification-dependent macromolecule catabolic process
proteolysis involved in cellular protein catabolic process
cellular protein catabolic process
protein catabolic process
protein ubiquitination
regulation of proteolysis
protein modification by small protein conjugation
regulation of cellular catabolic process
zinc ion binding
cellular macromolecule catabolic process
protein modification by small protein conjugation or removal
regulation of catabolic process
macromolecule catabolic process
organonitrogen compound catabolic process
proteolysis