POLD2

Gene Information

Official Symbol: POLD2
Official Name: DNA polymerase delta 2, accessory subunit
Aliases and Previous Symbols: N/A
Entrez ID: 5425
UniProt: P49005
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:12403614, PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol- delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS as a component of the POLZ complex. Along with POLD3, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906). {ECO:0000269|PubMed:12403614, ECO:0000269|PubMed:16510448, ECO:0000269|PubMed:19074196, ECO:0000269|PubMed:20227374, ECO:0000269|PubMed:20334433, ECO:0000269|PubMed:24035200, ECO:0000269|PubMed:24310611, ECO:0000269|PubMed:24449906}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
THZ1 0.06μM R08 exp531	-2.69
Quercetin 20μM R08 exp517	-2.23
ONC212 0.15μM R08 exp513	-2
Atovaquone 15μM R08 exp451	-1.96
DKK1 89ng/ml R08 exp477	-1.93
Bleomycin 5μM R08 exp459	-1.83
Etoposide 0.3μM R05 exp199	-1.82
TRAIL 5ng/ml R00 exp35	-1.77
Tunicamycin 0.04 to 0.075μM on day4 R07 exp419	1.76
Myriocin 25μM R06 exp293	1.82
Thalidomide 20μM R07 exp406	1.83
UM0131023 0.05μM R05 exp248	1.86
Adefovir 20μM R07 exp332	1.87
Ibrutinib 1μM R07 exp372	1.9
Alda-1 10μM R06 exp262	1.97
Deguelin 0.05μM R02 exp69	2.13
Momordin-Ic 10μM R08 exp506	2.19
Aminopterin 0.005μM R07 exp335	2.26
GW501516 25μM R06 exp285	2.27
Fas-L 44ng/ml R08 exp482	2.28
CCT251545 20μM R07 exp326	2.38
KU-0063794 3.8μM R02 exp71	2.45
FK-506 5μM R07 exp358	3.04

Gene	Correlation
STIL	0.447
KCTD17	0.401

Global Fraction of Cell Lines Where Essential: 166/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	0/1
bile duct	5/28
blood	8/28
bone	8/26
breast	6/33
central nervous system	16/56
cervix	1/4
colorectal	5/17
esophagus	4/13
fibroblast	0/1
gastric	4/16
kidney	2/21
liver	2/20
lung	11/75
lymphocyte	4/16
ovary	7/26
pancreas	3/24
peripheral nervous system	5/16
plasma cell	5/15
prostate	0/1
skin	0/24
soft tissue	2/9
thyroid	0/2
upper aerodigestive	7/22
urinary tract	6/29
uterus	3/5

Essentiality in NALM6

Essentiality Rank: 84
Expression level (log2 read counts): 6.43

Expression Distribution