TAF1
Gene Information
- Official Symbol: TAF1
- Official Name: TATA-box binding protein associated factor 1
- Aliases and Previous Symbols: N/A
- Entrez ID: 6872
- UniProt: P21675
- Interactions: BioGRID
- PubMed articles: Open PubMed
- OMIM: Open OMIM
Function Summary
- Entrez Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013].
- UniProt Summary: N/A
Pfam Domains GO Terms
Pfam Domains
| Bromodomain |
| TBP-binding |
| DUF3591 |
GO Terms
| negative regulation of protein autoubiquitination |
| transcription factor catabolic process |
| negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage |
| H3K27me3 modified histone binding |
| acetyl-CoA binding |
| positive regulation of androgen receptor activity |
| RNA polymerase II-specific DNA-binding transcription factor binding |
| regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage |
| regulation of protein autoubiquitination |
| negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding |
| regulation of RNA polymerase II regulatory region sequence-specific DNA binding |
| RNA polymerase II general transcription initiation factor activity |
| RNA polymerase II preinitiation complex assembly |
| cellular response to ATP |
| positive regulation of transcription initiation from RNA polymerase II promoter |
| lysine-acetylated histone binding |
| negative regulation of transcription regulatory region DNA binding |
| TBP-class protein binding |
| positive regulation of transcription by RNA polymerase I |
| positive regulation of DNA-templated transcription, initiation |
| nuclear hormone receptor binding |
| regulation of transcription initiation from RNA polymerase II promoter |
| transcription preinitiation complex assembly |
| MLL1 complex |
| response to ATP |
| regulation of transcription by RNA polymerase I |
| regulation of DNA-templated transcription, initiation |
| transcription factor TFIID complex |
| ubiquitin conjugating enzyme activity |
| negative regulation of ubiquitin-dependent protein catabolic process |
| histone acetyltransferase activity |
| regulation of transcription regulatory region DNA binding |
| negative regulation of DNA binding |
| p53 binding |
| cellular response to nutrient |
| negative regulation of proteolysis involved in cellular protein catabolic process |
| negative regulation of protein ubiquitination |
| kinase activity |
| peptidyl-threonine phosphorylation |
| positive regulation of proteasomal ubiquitin-dependent protein catabolic process |
| negative regulation of protein modification by small protein conjugation or removal |
| cellular response to UV |
| midbrain development |
| negative regulation of cellular protein catabolic process |
| peptidyl-threonine modification |
| positive regulation of protein binding |
| positive regulation of ubiquitin-dependent protein catabolic process |
| positive regulation of proteasomal protein catabolic process |
| cellular response to light stimulus |
| histone acetylation |
| internal peptidyl-lysine acetylation |
| regulation of transcription from RNA polymerase II promoter in response to stress |
| positive regulation of proteolysis involved in cellular protein catabolic process |
| peptidyl-lysine acetylation |
| internal protein amino acid acetylation |
| regulation of proteasomal ubiquitin-dependent protein catabolic process |
| regulation of DNA-templated transcription in response to stress |
| regulation of DNA binding |
| negative regulation of protein catabolic process |
| response to organophosphorus |
| positive regulation of cellular protein catabolic process |
| response to UV |
| protein acetylation |
| response to purine-containing compound |
| regulation of ubiquitin-dependent protein catabolic process |
| negative regulation of DNA-binding transcription factor activity |
| regulation of cell cycle G1/S phase transition |
| negative regulation of binding |
| cellular response to radiation |
| peptidyl-serine phosphorylation |
| transcription initiation from RNA polymerase II promoter |
| positive regulation of binding |
| protein stabilization |
| regulation of signal transduction by p53 class mediator |
| regulation of proteasomal protein catabolic process |
| protein acylation |
| protein autophosphorylation |
| peptidyl-serine modification |
| regulation of protein ubiquitination |
| protein-DNA complex assembly |
| transcription factor complex |
| response to nutrient |
| regulation of proteolysis involved in cellular protein catabolic process |
| DNA-templated transcription, initiation |
| positive regulation of protein catabolic process |
| transcription regulatory region DNA binding |
| regulation of protein binding |
| regulation of protein modification by small protein conjugation or removal |
| cellular response to nutrient levels |
| nuclear chromatin |
| protein-DNA complex subunit organization |
| regulation of cellular protein catabolic process |
| negative regulation of cellular catabolic process |
| positive regulation of DNA-binding transcription factor activity |
| cellular response to extracellular stimulus |
| transcription coactivator activity |
| regulation of protein stability |
| protein polyubiquitination |
| response to light stimulus |
| negative regulation of catabolic process |
| peptidyl-lysine modification |
| cellular response to abiotic stimulus |
| cellular response to environmental stimulus |
| proteasome-mediated ubiquitin-dependent protein catabolic process |
| transcription factor binding |
| cellular response to external stimulus |
| proteasomal protein catabolic process |
| negative regulation of proteolysis |
| positive regulation of proteolysis |
| protein serine/threonine kinase activity |
| histone modification |
| positive regulation of cellular catabolic process |
| covalent chromatin modification |
| regulation of binding |
| regulation of protein catabolic process |
| cellular response to drug |
| sequence-specific DNA binding |
| regulation of DNA-binding transcription factor activity |
| positive regulation of catabolic process |
| response to radiation |
| regulation of cell cycle phase transition |
| transcription by RNA polymerase II |
| protein heterodimerization activity |
| response to nutrient levels |
| ubiquitin-dependent protein catabolic process |
| response to extracellular stimulus |
| modification-dependent protein catabolic process |
| cellular response to organic cyclic compound |
| modification-dependent macromolecule catabolic process |
| proteolysis involved in cellular protein catabolic process |
| negative regulation of protein modification process |
| cellular response to organonitrogen compound |
| cellular protein catabolic process |
| transcription, DNA-templated |
| nucleic acid-templated transcription |
| RNA biosynthetic process |
| cellular response to nitrogen compound |
| protein catabolic process |
| protein ubiquitination |
| chromatin organization |
| viral process |
| regulation of proteolysis |
| brain development |
| regulation of cell cycle process |
| protein modification by small protein conjugation |
| cellular response to DNA damage stimulus |
| head development |
| symbiotic process |
| interspecies interaction between organisms |
| regulation of cellular catabolic process |
| cellular protein-containing complex assembly |
| nucleolus |
| negative regulation of transcription by RNA polymerase II |
| peptidyl-amino acid modification |
| cellular macromolecule catabolic process |
| response to organic cyclic compound |
| protein phosphorylation |
| central nervous system development |
| protein modification by small protein conjugation or removal |
| regulation of catabolic process |
| response to organonitrogen compound |
| response to drug |
| negative regulation of cellular protein metabolic process |
| macromolecule catabolic process |
| cellular response to oxygen-containing compound |
| organonitrogen compound catabolic process |
| chromosome organization |
| response to nitrogen compound |
| nucleobase-containing compound biosynthetic process |
| negative regulation of protein metabolic process |
| negative regulation of molecular function |
| response to abiotic stimulus |
| heterocycle biosynthetic process |
| aromatic compound biosynthetic process |
| regulation of cell cycle |
| negative regulation of transcription, DNA-templated |
| positive regulation of transcription by RNA polymerase II |
| cellular response to endogenous stimulus |
| negative regulation of nucleic acid-templated transcription |
| negative regulation of RNA biosynthetic process |
| proteolysis |
| phosphorylation |
| organic cyclic compound biosynthetic process |
| negative regulation of RNA metabolic process |
| cell cycle |
| negative regulation of cellular macromolecule biosynthetic process |
| negative regulation of nucleobase-containing compound metabolic process |
| negative regulation of macromolecule biosynthetic process |
| response to endogenous stimulus |
| ATP binding |
| negative regulation of cellular biosynthetic process |
| positive regulation of transcription, DNA-templated |
| negative regulation of biosynthetic process |
| response to oxygen-containing compound |
| protein-containing complex assembly |
| DNA-binding transcription factor activity, RNA polymerase II-specific |
| positive regulation of cellular protein metabolic process |
| cellular nitrogen compound biosynthetic process |
| positive regulation of nucleic acid-templated transcription |
| positive regulation of RNA biosynthetic process |
| positive regulation of signal transduction |
| RNA metabolic process |
| cellular response to stress |
| positive regulation of protein metabolic process |
| cellular macromolecule biosynthetic process |
| negative regulation of gene expression |
| positive regulation of RNA metabolic process |
| macromolecule biosynthetic process |
| organic substance catabolic process |
| positive regulation of molecular function |
| cellular catabolic process |
| positive regulation of cell communication |
| positive regulation of signaling |
| regulation of intracellular signal transduction |
| regulation of protein modification process |
| protein-containing complex subunit organization |
| positive regulation of nucleobase-containing compound metabolic process |
| positive regulation of macromolecule biosynthetic process |
| positive regulation of cellular biosynthetic process |
| positive regulation of gene expression |
| gene expression |
| positive regulation of biosynthetic process |
CRISPR Data
Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Compound Hit
Most Correlated Genes in Chemogenomics
| Gene | Correlation |
|---|---|
| POLR2J3 | 0.601 |
| GOLGA6L1 | 0.598 |
| AATF | 0.519 |
| PSMB4 | 0.515 |
| BRD2 | 0.507 |
| PRIM1 | 0.501 |
| TCEB3CL | 0.491 |
| TCEB3C | 0.483 |
| MYB | 0.48 |
| NOP16 | 0.479 |
| TCEB3CL2 | 0.476 |
| PSMA7 | 0.474 |
| GOLGA6L6 | 0.465 |
| PSMB1 | 0.451 |
| RPS7 | 0.449 |
| RPL35 | 0.449 |
| VPS54 | 0.447 |
| SLC6A17 | 0.445 |
| NOL12 | 0.436 |
| PSMA6 | 0.433 |
| COX7A2L | 0.431 |
| RANBP2 | 0.43 |
| CHD4 | 0.426 |
| MRS2 | 0.426 |
| RPS19 | 0.426 |
| PAN3 | 0.423 |
| NARFL | 0.423 |
| ORC1 | 0.422 |
| SMG1 | 0.42 |
| COPG1 | 0.417 |
| AKIRIN2 | 0.417 |
| GID4 | 0.417 |
| RPS12 | 0.415 |
| WDR75 | 0.415 |
| DIDO1 | 0.414 |
| TERF2 | 0.41 |
| AAMP | 0.41 |
| TSG101 | 0.41 |
| TYMS | 0.41 |
| SUPT6H | 0.41 |
| SMG7 | 0.409 |
| RPS3A | 0.409 |
| PRPF3 | 0.407 |
| POLR2D | 0.406 |
| NIP7 | 0.404 |
| PSMB2 | 0.402 |
| PPM1D | 0.401 |
| SF3A1 | 0.401 |
| TSPY8 | 0.401 |
| RPL14 | 0.4 |
Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Global Fraction of Cell Lines Where Essential: 5/683
| Tissue | Fraction Of Cell Lines Where Essential |
|---|---|
| 1290807.0 | 0/1 |
| 909776.0 | 0/1 |
| bile duct | 0/28 |
| blood | 0/26 |
| bone | 0/25 |
| breast | 2/30 |
| central nervous system | 0/49 |
| cervix | 0/4 |
| colorectal | 1/17 |
| esophagus | 0/11 |
| fibroblast | 0/1 |
| gastric | 0/14 |
| kidney | 0/18 |
| liver | 0/19 |
| lung | 0/72 |
| lymphocyte | 0/14 |
| ovary | 0/25 |
| pancreas | 0/22 |
| peripheral nervous system | 0/15 |
| plasma cell | 1/12 |
| prostate | 0/1 |
| skin | 0/20 |
| soft tissue | 0/7 |
| thyroid | 0/2 |
| upper aerodigestive | 0/22 |
| urinary tract | 0/28 |
| uterus | 0/5 |
Essentiality in NALM6
- Essentiality Rank: 747
- Expression level (log2 read counts): 6.83
Expression Distribution
TAF1 Expression in NALM6 Cells: 6.83
