DABN 20μM R00 exp17

DABN 20μM R00 exp17

Mechanism of Action

Unknown

Class / Subclass 1: Uncharacterized Mechanism / Chemical Screen Hit

Technical Notes

Protein References

PubChem Name: [1,1'-Binaphthalene]-2,2'-diamine
Synonyms: N/A
CAS #: 4488-22-6
PubChem CID: 20571
IUPAC: 1-(2-aminonaphthalen-1-yl)naphthalen-2-amine
INCHI Name: InChI=1S/C20H16N2/c21-17-11-9-13-5-1-3-7-15(13)19(17)20-16-8-4-2-6-14(16)10-12-18(20)22/h1-12H,21-22H2
INCHI Key: DDAPSNKEOHDLKB-UHFFFAOYSA-N
Molecular Weight: 284.4
Canonical SMILES: C1=CC=C2C(=C1)C=CC(=C2C3=C(C=CC4=CC=CC=C43)N)N
Isomeric SMILES: N/A
Molecular Formula: C20H16N2

Protein Supplier

Supplier Name: Chem-Impex International
Catalog #: 24623
Lot #: 001702-20140303

Protein Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C20H16N2 285.13862; found 285.13894

Dose Response Curve

Platform ID: DABN
Min: -10.2228; Max: 85.8979

IC	Concentration (µM)
IC10	N/A
IC20	6.0249
IC30	6.1377
IC40	6.2318
IC50	6.3193
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 00
Dose: 20µM
Days of incubation: 8
Doublings: 0.4
Numbers of reads: 11551454

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
10/9	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
ATP6V1A	-3.93	<0.01
ATP6V1B2	-3.88	<0.01
SPTLC2	-3.71	<0.01
AURKB	-3.51	0.02
ATP6V0A2	-3.40	<0.01
PDS5B	-3.35	<0.01
DDX3X	-3.09	<0.01
CNOT2	-3.00	<0.01
PRAMEF25	-2.93	0.30
PRAMEF26	-2.91	0.30
EP300	-2.89	0.11
BRD2	-2.88	<0.01
ATP6AP1	-2.77	0.35
XAB2	-2.74	0.12
SRBD1	-2.70	0.12
PSMB4	-2.67	0.08
LRP10	-2.59	0.09
HNRNPCL1	-2.53	0.10
ATP6V0D1	-2.53	0.12
COPA	-2.53	0.21
BAGE	-2.52	0.44
BAGE3	-2.51	0.44
PRIM1	-2.51	0.44
BAGE2	-2.50	0.44
BAGE5	-2.49	0.44
BAGE4	-2.48	0.44
EGFL8	-2.47	0.04
INCENP	-2.44	0.09
RNF103-CHMP3	-2.42	0.09
ATP6V0B	-2.41	0.11
TLE4	2.05	0.39
ITIH2	2.09	0.25
HSPD1	2.09	0.16
RIMS2	2.12	0.15
MED23	2.14	0.14
CSNK2B	2.15	0.23
SKIL	2.16	0.14
ATG16L1	2.18	0.13
TRIAP1	2.22	0.13
PABPC4	2.24	0.13
LDB2	2.24	0.15
CRLS1	2.25	0.13
GPC3	2.27	0.12
MTOR	2.29	0.23
CCDC117	2.32	0.13
BTBD9	2.32	0.13
TIMM23B	2.35	0.39
RBM14-RBM4	2.43	0.14
RBM14	2.44	0.14
RAD1	2.44	0.05
APAF1	2.73	0.01
HCCS	2.73	0.06
PELO	2.91	<0.01
MAD1L1	3.33	<0.01
BUB1B	3.77	<0.01
ZW10	4.17	<0.01
MAD2L1	4.79	<0.01
BUB3	4.85	<0.01
TRIP13	5.07	<0.01
KNTC1	6.66	<0.01

Screen	Correlation	Plot
Pimelic-diphenylamide-106 5μM R00 exp28	0.113	View
Nocodazole 0.2μM R00 exp24	0.111	View
Etoposide 1μM R00 exp19	0.11	View
Rotenone 2μM R00 exp33	0.094	View
Oligomycin-A 20μM R00 exp26	0.092	View
MLN-4924 2μM R00 exp22	0.083	View
Rotenone 20μM R00 exp34	0.079	View
Nocodazole 0.2μM R02 exp64	0.078	View
THZ1 0.06μM R08 exp531	0.074	View
Vinblastine 0.015μM R05 exp208	0.074	View
Vinblastine 0.2μM R03 exp108	0.071	View
GSK461364A 0.1μM R03 exp126	0.057	View
HMS-I1 10μM R06 exp256	0.056	View
Etoposide 0.3μM R05 exp199	0.054	View
PU-H71 1μM R08 exp515	0.053	View
R-DABN 8μM R03 exp111	0.053	View
DABN racemic mixture R03 exp93	0.052	View
Doxorubicin 0.02μM R08 exp478	0.051	View
Bortezomib 0.05μM R00 exp7	0.119	View
5-Fluorouracil 20μM R00 exp2	0.098	View
BI-6727 0.04μM R03 exp98	0.066	View
Actinomycin-D 0.001μM R00 exp3	0.064	View
Brefeldin A 0.02μM R00 exp8	0.056	View
Actinomycin-D 0.01μM R00 exp4	0.056	View
BI-2536 0.02μM R03 exp96	0.053	View
Centrinone 0.5μM R07 exp343	0.052	View

GO Term	Fold Change	Genes
cellular response to increased oxygen levels	205.55	ATP6V1A,ATP6V0A2,ATP6AP1
synaptic vesicle lumen acidification	200.98	ATP6V1A,ATP6V1B2,ATP6AP1,ATP6V0D1
proton-transporting V-type ATPase complex	139.57	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1
ATPase-coupled ion transmembrane transporter activity	131.08	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6V0D1
proton-transporting ATPase activity, rotational mechanism	131.08	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6V0D1
ATPase activity, coupled to transmembrane movement of ions, rotational mechanism	131.08	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6V0D1
vacuolar proton-transporting V-type ATPase complex	125.61	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6V0D1
response to increased oxygen levels	120.59	ATP6V1A,ATP6V0A2,ATP6AP1,ATP6V0D1
vacuolar acidification	114.19	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1
proton-transporting two-sector ATPase complex	83.74	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1
intracellular pH reduction	73.89	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1
ATPase-coupled monoatomic cation transmembrane transporter activity	59.11	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6V0D1
intracellular iron ion homeostasis	44.33	ATP6V1A,ATP6V0A2,ATP6AP1,ATP6V0D1
regulation of intracellular pH	43.32	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1
regulation of cellular pH	40.96	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1
regulation of pH	36.95	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1
proton transmembrane transport	28.55	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1
ATPase complex	26.92	ATP6V1A,ATP6V1B2,ATP6V0A2,ATP6AP1,ATP6V0D1
coated vesicle membrane	19.53	ATP6V1A,ATP6V1B2,ATP6AP1,ATP6V0D1,COPA

GO Term	Fold Change	Genes
mitotic spindle assembly checkpoint signaling	143.29	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
spindle assembly checkpoint signaling	143.29	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
mitotic spindle checkpoint signaling	143.29	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
negative regulation of mitotic sister chromatid segregation	128.96	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
negative regulation of mitotic sister chromatid separation	128.96	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
negative regulation of chromosome segregation	120.90	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
negative regulation of metaphase/anaphase transition of cell cycle	120.90	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
negative regulation of chromosome separation	120.90	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
spindle checkpoint signaling	118.44	KNTC1,TRIP13,BUB3,ZW10,BUB1B,MAD1L1
negative regulation of sister chromatid segregation	110.54	KNTC1,TRIP13,BUB3,ZW10,BUB1B,MAD1L1
negative regulation of mitotic metaphase/anaphase transition	110.54	KNTC1,TRIP13,MAD2L1,ZW10,BUB1B,MAD1L1
negative regulation of nuclear division	98.26	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1,RAD1
regulation of mitotic sister chromatid segregation	73.00	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
regulation of chromosome separation	53.73	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
negative regulation of chromosome organization	52.28	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1
mitotic cell cycle checkpoint signaling	36.54	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1,TRIAP1
cell cycle checkpoint signaling	32.09	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1,RAD1,TRIAP1
negative regulation of cell cycle process	21.18	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1,RAD1,RBM14,TRIAP1
negative regulation of organelle organization	18.82	KNTC1,TRIP13,BUB3,MAD2L1,ZW10,BUB1B,MAD1L1,RAD1,RBM14,MTOR,TRIAP1

Table of Contents

DABN 20μM R00 exp17

Mechanism of Action

Technical Notes

Protein References

Protein Supplier

Protein Characterization

Dose Response Curve

Screen Summary

Screen Results