SB743921 0.001μM R05 exp244

Inhibits Eg5 kinesin, derivative of S-trityl-L-cysteine

Class / Subclass 1: Cell Cycle / Mitotic Inhibitor

Compound References

PubChem Name: N-(3-Aminopropyl)-N-[(1R)-1-(3-benzyl-7-chloro-4-oxochromen-2-yl)-2-methylpropyl]-4-methylbenzamide;hydrochloride
Synonyms: N/A
CAS #: 940929-33-9
PubChem CID: 49867937
IUPAC: N-(3-aminopropyl)-N-[(1R)-1-(3-benzyl-7-chloro-4-oxochromen-2-yl)-2-methylpropyl]-4-methylbenzamide;hydrochloride
INCHI Name: InChI=1S/C31H33ClN2O3.ClH/c1-20(2)28(34(17-7-16-33)31(36)23-12-10-21(3)11-13-23)30-26(18-22-8-5-4-6-9-22)29(35)25-15-14-24(32)19-27(25)37-30;/h4-6,8-15,19-20,28H,7,16-18,33H2,1-3H3;1H/t28-;/m1./s1
INCHI Key: MLMZVWABFOLFGV-LNLSOMNWSA-N
Molecular Weight: 553.5
Canonical SMILES: CC1=CC=C(C=C1)C(=O)N(CCCN)C(C2=C(C(=O)C3=C(O2)C=C(C=C3)Cl)CC4=CC=CC=C4)C(C)C.Cl
Isomeric SMILES: CC1=CC=C(C=C1)C(=O)N(CCCN)[C@@H](C2=C(C(=O)C3=C(O2)C=C(C=C3)Cl)CC4=CC=CC=C4)C(C)C.Cl
Molecular Formula: C31H34Cl2N2O3

Compound Supplier

Supplier Name: N/A
Catalog #: N/A
Lot #: N/A

Compound Characterization

Characterization data not available.

Dose Response Curve

Platform ID: SB743921
Min: -3.7906; Max: 93.6518

IC	Concentration (µM)
IC10	N/A
IC20	0.0006
IC30	0.0007
IC40	0.0007
IC50	0.0008
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 05
Dose: 1nM
Days of incubation: 8
Doublings: -0.0
Numbers of reads: 22832707

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
0/155	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
COPA	-2.95	0.31
AURKB	-2.87	0.31
TPX2	-2.82	0.24
PSMC5	-2.81	0.31
SMC2	-2.69	0.31
UBE2Z	-2.61	0.21
TCERG1L	-2.50	0.24
TSC2	-2.47	0.22
SKIV2L2	-2.40	0.57
OR2A14	-2.36	0.24
TBCC	-2.34	0.38
CDC73	-2.32	0.33
PPP1R13L	-2.31	0.31
PSMC6	-2.28	0.54
ZNF202	-2.26	0.31
KRTAP4-8	-2.19	0.38
CTR9	-2.18	0.38
GSN	-2.17	0.31
PTPRJ	-2.17	0.31
C1orf173	-2.11	0.31
RBKS	-2.09	0.31
DNAJC21	-2.09	0.31
C1orf131	-2.07	0.38
CEP250	-2.07	0.31
ASH2L	-2.07	0.31
DONSON	-2.07	0.69
COG3	-2.07	0.38
ANKRD12	-2.07	0.31
CHMP6	-2.06	0.31
ANKRD34A	-2.05	0.31
PDE1C	3.47	<0.01
IGF2BP3	3.47	<0.01
RADIL	3.47	<0.01
HOXA1	3.49	<0.01
HDAC9	3.51	<0.01
NT5C3A	3.53	<0.01
DCTN5	3.69	<0.01
INMT	3.76	<0.01
BZW2	4.09	<0.01
RAC1	4.13	<0.01
TNRC18	4.15	<0.01
MAD2L1BP	4.16	<0.01
USP42	4.31	<0.01
RBM14-RBM4	4.41	<0.01
CYTH3	4.43	<0.01
RBM14	4.56	<0.01
ITGB8	4.67	<0.01
AMZ1	4.69	<0.01
BMPER	4.74	<0.01
ZWILCH	4.93	<0.01
PAFAH1B1	5.04	<0.01
MAD1L1	5.20	<0.01
DYNLRB1	5.54	<0.01
DYNC1H1	5.70	<0.01
BUB3	5.75	<0.01
BUB1B	5.87	<0.01
MAD2L1	6.04	<0.01
ZW10	6.17	<0.01
KNTC1	6.56	<0.01
TRIP13	7.14	<0.01

Screen	Correlation	Plot
Vinblastine 0.015μM R05 exp208	0.291	View
Nocodazole 0.1μM R03 exp94	0.341	View
Rotenone 2μM R00 exp33	0.338	View
Nocodazole 0.2μM R00 exp24	0.311	View
DABN racemic mixture R03 exp93	0.273	View
R-DABN 8μM R03 exp111	0.227	View
Nocodazole 0.2μM R02 exp64	0.221	View
HMS-I1 10μM R06 exp256	0.216	View
BI-6727 0.04μM R03 exp98	0.091	View

GO Term	Fold Change	Genes
mitotic spindle checkpoint signaling	157.81	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH
spindle assembly checkpoint signaling	157.81	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH
mitotic spindle assembly checkpoint signaling	157.81	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH
negative regulation of mitotic sister chromatid segregation	142.54	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH
negative regulation of mitotic sister chromatid separation	142.54	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH
negative regulation of metaphase/anaphase transition of cell cycle	133.90	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH
negative regulation of chromosome separation	133.90	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH
negative regulation of chromosome segregation	133.90	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH
spindle checkpoint signaling	133.32	TRIP13,KNTC1,ZW10,BUB1B,BUB3,MAD1L1,ZWILCH
negative regulation of mitotic metaphase/anaphase transition	124.72	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,MAD1L1,ZWILCH
negative regulation of sister chromatid segregation	124.72	TRIP13,KNTC1,ZW10,BUB1B,BUB3,MAD1L1,ZWILCH
negative regulation of nuclear division	96.06	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH
regulation of mitotic sister chromatid segregation	93.79	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH,MAD2L1BP
regulation of mitotic sister chromatid separation	77.52	TRIP13,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH,MAD2L1BP
regulation of chromosome separation	69.04	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH,MAD2L1BP
regulation of mitotic metaphase/anaphase transition	56.49	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH,MAD2L1BP
regulation of metaphase/anaphase transition of cell cycle	54.63	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH,MAD2L1BP
regulation of sister chromatid segregation	48.74	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH,MAD2L1BP
regulation of mitotic nuclear division	42.85	TRIP13,KNTC1,ZW10,MAD2L1,BUB1B,BUB3,MAD1L1,ZWILCH,MAD2L1BP

SB743921 0.001μM R05 exp244

Mechanism of Action

Technical Notes

Compound References

Compound Supplier

Compound Characterization

Dose Response Curve

Screen Summary

Screen Results