PCNA

Gene Information

Official Symbol: PCNA
Official Name: proliferating cell nuclear antigen
Aliases and Previous Symbols: N/A
Entrez ID: 5111
UniProt: P12004
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008].
UniProt Summary: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. {ECO:0000269|PubMed:18719106, ECO:0000269|PubMed:19443450, ECO:0000269|PubMed:24939902}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
AICAR 240μM R05 exp211	-2.14
Cidofovir 10μM R07 exp345	-2.11
Combretastatin A4 0.002 to 0.003μM day4 R04 exp179	-1.74
Vinblastine 0.2μM R03 exp108	-1.73
UNC2400 2μM R03 exp156	1.71
Chlorpromazine 10μM R07 exp344	1.73
Epigallocatechin gallate 20μM R05 exp230	1.76
Dexamethasone 0.006 to 0.015μM on day4 R07 exp352	1.81
Asunaprenir 3μM R07 exp336	1.82
JQ1 0.01μM R03 exp130	2.01
Ibrutinib 1μM R07 exp372	2.09
Dexamethasone 0.006μM R07 exp351	2.11
VHL-ligand-1 20μM R07 exp421	2.15
A-395N 10μM R05 exp219	2.21
PFI-3 10μM R03 exp144	2.33
Losmapimod 1μM R07 exp376	2.47

Global Fraction of Cell Lines Where Essential: 724/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	25/25
breast	33/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	15/15
kidney	21/21
liver	20/20
lung	75/75
lymphocyte	14/14
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	6/7
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 12
Expression level (log2 read counts): 8.38

Expression Distribution

Gene	Correlation
GOLGA6L1	0.708
PRIM1	0.466

PCNA N
Rad1
Rad9
PCNA C

PCNA complex
DNA polymerase processivity factor activity
replisome
PCNA-p21 complex
purine-specific mismatch base pair DNA N-glycosylase activity
dinucleotide insertion or deletion binding
leading strand elongation
mitotic telomere maintenance via semi-conservative replication
positive regulation of deoxyribonuclease activity
base-excision repair, gap-filling
MutLalpha complex binding
nuclear lamina
positive regulation of nuclease activity
regulation of deoxyribonuclease activity
response to L-glutamate
nuclear replication fork
DNA strand elongation involved in DNA replication
estrous cycle
DNA polymerase binding
error-prone translesion synthesis
error-free translesion synthesis
DNA strand elongation
regulation of nuclease activity
nucleotide-excision repair, DNA gap filling
telomere maintenance via semi-conservative replication
replication fork
regulation of transcription involved in G1/S transition of mitotic cell cycle
histone acetyltransferase binding
cyclin-dependent protein kinase holoenzyme complex
liver regeneration
replication fork processing
mismatch repair
positive regulation of DNA replication
nucleotide-excision repair, DNA incision, 5-to lesion
DNA damage response, detection of DNA damage
base-excision repair
DNA-dependent DNA replication maintenance of fidelity
response to dexamethasone
nucleotide-excision repair, DNA incision
translesion synthesis