EIF3E

Gene Information

Official Symbol: EIF3E
Official Name: eukaryotic translation initiation factor 3 subunit E
Aliases and Previous Symbols: N/A
Entrez ID: 3646
UniProt: P60228
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF- 3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway (PubMed:17468741). May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (PubMed:17310990, PubMed:17324924). {ECO:0000255|HAMAP-Rule:MF_03004, ECO:0000269|PubMed:17310990, ECO:0000269|PubMed:17324924, ECO:0000269|PubMed:17468741, ECO:0000269|PubMed:17581632, ECO:0000269|PubMed:25849773, ECO:0000269|PubMed:27462815}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Phorbol-12-myristate-13-acetate 0.57μM R08 exp514	-1.71
MLN-4924 0.2μM R00 exp21	1.74
Bisphenol S 100μM R08 exp458	1.75
UM0011500 5μM R05 exp245	1.75
THZ531 0.11 to 0.175μM on day4 R07 exp413	1.76
Nicotine 3000μM R08 exp510	1.77
Lead acetate 2000μM R08 exp498 no dilution day6	1.79
Nicotinamide Riboside 100μM R03 exp139	1.81
THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412	1.81
Celastrol 0.12μM R05 exp225	1.82
Doxycycline 10μM R00 exp18	1.85
Geldanamycin 0.015 to 0.05μM on day4 R07 exp317	1.86
QNZ 0.01μM R05 exp241	1.93
BI-6727 0.0125μM R03 exp97	1.95
BI-2536 0.0042μM R03 exp95	2.01
IU1-47 25μM R04 exp182	2.01
UMK57 0.6μM R03 exp107	2.05
Golgicide-A 4μM R03 exp122	2.08
SGC0946 7μM R03 exp151	2.14
Bortezomib 0.005μM R00 exp6	2.21
Dimethyl-Sulfoxide 1pc R03 exp120	2.22
Nifuroxazide 5μM R03 exp102	2.23
Epothilone-B 0.0015μM R05 exp231	2.24
Citral 50μM R06 exp274	2.28
Quinacrine 2.5μM R03 exp146	2.28
THZ531 0.225μM R08 exp444	3.05

Global Fraction of Cell Lines Where Essential: 707/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	26/28
blood	27/28
bone	23/26
breast	25/33
central nervous system	54/56
cervix	4/4
colorectal	17/17
esophagus	12/13
fibroblast	1/1
gastric	16/16
kidney	20/21
liver	20/20
lung	72/75
lymphocyte	16/16
ovary	26/26
pancreas	23/24
peripheral nervous system	16/16
plasma cell	14/15
prostate	1/1
skin	24/24
soft tissue	8/9
thyroid	2/2
upper aerodigestive	20/22
urinary tract	28/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 1156
Expression level (log2 read counts): 7.96

Expression Distribution

PCI
eIF3 N

eukaryotic translation initiation factor 3 complex, eIF3e
positive regulation of mRNA binding
positive regulation of RNA binding
regulation of mRNA binding
regulation of RNA binding
eukaryotic 48S preinitiation complex
formation of cytoplasmic translation initiation complex
eukaryotic translation initiation factor 3 complex
eukaryotic 43S preinitiation complex
negative regulation of translational initiation
cytoplasmic translational initiation
translation initiation factor activity
cytoplasmic translation
regulation of translational initiation
PML body
protein N-terminus binding
chromatin
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
positive regulation of translation
negative regulation of translation
translational initiation
negative regulation of cellular amide metabolic process
positive regulation of cellular amide metabolic process
positive regulation of binding
nuclear-transcribed mRNA catabolic process
mRNA catabolic process
ribonucleoprotein complex assembly
ribonucleoprotein complex subunit organization
RNA catabolic process
nuclear body
cadherin binding
regulation of translation
nucleobase-containing compound catabolic process
regulation of binding
translation
regulation of cellular amide metabolic process
peptide biosynthetic process
heterocycle catabolic process
cellular nitrogen compound catabolic process
aromatic compound catabolic process