LARS
Gene Information
- Official Symbol: LARS1
- Official Name: leucyl-tRNA synthetase 1
- Aliases and Previous Symbols: N/A
- Entrez ID: 51520
- UniProt: Q9P2J5
- Interactions: BioGRID
- PubMed articles: Open PubMed
- OMIM: Open OMIM
Function Summary
- Entrez Summary: N/A
- UniProt Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs. {ECO:0000269|PubMed:19426743}.
Pfam Domains GO Terms
Pfam Domains
| tRNA-synt 1e |
| tRNA-synt 1g |
| Anticodon 1 |
| tRNA-synt 1 |
GO Terms
| leucine-tRNA ligase activity |
| glutaminyl-tRNA aminoacylation |
| glutamine-tRNA ligase activity |
| leucyl-tRNA aminoacylation |
| cellular response to leucine |
| cellular response to leucine starvation |
| aminoacyl-tRNA synthetase multienzyme complex |
| response to leucine |
| aminoacyl-tRNA metabolism involved in translational fidelity |
| aminoacyl-tRNA editing activity |
| positive regulation of TORC1 signaling |
| protein targeting to lysosome |
| regulation of translational fidelity |
| protein targeting to vacuole |
| protein localization to lysosome |
| regulation of TORC1 signaling |
| positive regulation of TOR signaling |
| cellular response to amino acid starvation |
| tRNA aminoacylation for protein translation |
| response to amino acid starvation |
| establishment of protein localization to vacuole |
| tRNA aminoacylation |
| amino acid activation |
| protein localization to vacuole |
| cellular response to amino acid stimulus |
| endomembrane system |
| negative regulation of autophagy |
| regulation of TOR signaling |
| lysosomal transport |
| response to amino acid |
| vacuolar transport |
| cellular response to starvation |
| regulation of cell size |
| tRNA metabolic process |
| response to starvation |
| cellular response to acid chemical |
| cellular response to nutrient levels |
| lysosome |
| negative regulation of cellular catabolic process |
| cellular response to extracellular stimulus |
| GTPase activator activity |
| nuclear body |
| negative regulation of catabolic process |
| cellular amino acid metabolic process |
| regulation of autophagy |
| cellular response to external stimulus |
| response to acid chemical |
| protein targeting |
| regulation of cellular component size |
| translation |
| peptide biosynthetic process |
| positive regulation of GTPase activity |
| establishment of protein localization to organelle |
| ncRNA metabolic process |
| regulation of GTPase activity |
| response to nutrient levels |
| regulation of anatomical structure size |
| amide biosynthetic process |
| peptide metabolic process |
| response to extracellular stimulus |
| cellular response to organonitrogen compound |
| cellular response to nitrogen compound |
| protein localization to organelle |
| positive regulation of hydrolase activity |
| cellular amide metabolic process |
| regulation of cellular catabolic process |
| carboxylic acid metabolic process |
| regulation of catabolic process |
| intracellular protein transport |
| response to organonitrogen compound |
| oxoacid metabolic process |
| endoplasmic reticulum |
| positive regulation of intracellular signal transduction |
| organic acid metabolic process |
| cellular response to oxygen-containing compound |
| response to nitrogen compound |
| cellular response to endogenous stimulus |
| regulation of hydrolase activity |
| organonitrogen compound biosynthetic process |
| positive regulation of catalytic activity |
| response to endogenous stimulus |
| ATP binding |
| protein transport |
| intracellular transport |
| peptide transport |
| response to oxygen-containing compound |
| amide transport |
| cellular protein localization |
| cellular macromolecule localization |
| establishment of protein localization |
| cellular nitrogen compound biosynthetic process |
| positive regulation of signal transduction |
| RNA metabolic process |
| cellular response to stress |
| cellular macromolecule biosynthetic process |
| small molecule metabolic process |
| macromolecule biosynthetic process |
| positive regulation of molecular function |
| positive regulation of cell communication |
| positive regulation of signaling |
| regulation of intracellular signal transduction |
| establishment of localization in cell |
| nitrogen compound transport |
| gene expression |
CRISPR Data
Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Compound Hit
| Screen | Score |
|---|---|
| Mitomycin-C 0.06μM R08 exp503 | -2.65 |
| Rucaparib 6.5μM R08 exp520 | -2.33 |
| KU-0063794 3.8μM R02 exp71 | -1.85 |
| AICAR 240μM R03 exp116 | -1.82 |
| TIC10 10μM R08 exp532 | -1.81 |
| A-366 10μM R03 exp115 | 1.82 |
| GSK-J5 1.5μM R08 exp484 | 1.87 |
| Nifuroxazide 1μM R01 exp51 | 1.89 |
| Hydroxyurea 15μM R06 exp289 | 2.36 |
| IU1-C 25μM R04 exp183 | 2.53 |
Most Correlated Genes in Chemogenomics
| Gene | Correlation |
|---|---|
| RRM1 | 0.442 |
Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Global Fraction of Cell Lines Where Essential: 171/739
| Tissue | Fraction Of Cell Lines Where Essential |
|---|---|
| 1290807.0 | 0/1 |
| 909776.0 | 0/1 |
| bile duct | 9/28 |
| blood | 8/28 |
| bone | 8/26 |
| breast | 6/33 |
| central nervous system | 9/56 |
| cervix | 1/4 |
| colorectal | 7/17 |
| esophagus | 2/13 |
| fibroblast | 0/1 |
| gastric | 2/16 |
| kidney | 4/21 |
| liver | 4/20 |
| lung | 19/75 |
| lymphocyte | 5/16 |
| ovary | 7/26 |
| pancreas | 4/24 |
| peripheral nervous system | 2/16 |
| plasma cell | 3/15 |
| prostate | 1/1 |
| skin | 8/24 |
| soft tissue | 3/9 |
| thyroid | 0/2 |
| upper aerodigestive | 2/22 |
| urinary tract | 5/29 |
| uterus | 1/5 |
Essentiality in NALM6
- Essentiality Rank: 74
- Expression level (log2 read counts): 7.21
Expression Distribution
LARS Expression in NALM6 Cells: 7.21
