PRPF4

Entrez Summary: The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016].
UniProt Summary: Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. {ECO:0000269|PubMed:25383878}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Cytochalasin-B 5μM R07 exp349	-2.15
Sulforaphane 9μM R08 exp526 no dilution day6	-2.11
Salinomycin 3.5μM R08 exp521	-2.06
ABT-702 5μM plus Dimethyloxaloylglycine 11μM R07 exp319	-1.93
Palbociclib 1μM R07 exp382	-1.88
Ammonium tetrathiomolybdate 10μM R08 exp448	-1.81
Epothilone-D 0.004 to 0.006μM on day4 R05 exp232	1.71
Mubritinib 0.2μM R02 exp65	1.73
Docosahexaenoic-acid 50μM R07 exp356	1.75
INK128 0.2μM R02 exp70	1.81
SGC0946 7μM R03 exp151	1.82
Apcin 50 to 100μM on day4 R04 exp176	1.9
JQ1 0.01μM R03 exp130	1.93
Myriocin 25μM R06 exp293	1.98
PFI-3 10μM R03 exp144	2.12
Trametinib 10μM R02 exp83	2.39
Isonicotinamide 500μM R03 exp129	2.43
Ethanol 0.01 R05 exp234	2.44

Global Fraction of Cell Lines Where Essential: 408/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	15/28
blood	18/28
bone	13/26
breast	16/33
central nervous system	25/56
cervix	1/4
colorectal	11/17
esophagus	4/13
fibroblast	0/1
gastric	10/16
kidney	10/21
liver	13/20
lung	41/75
lymphocyte	7/16
ovary	18/26
pancreas	17/24
peripheral nervous system	14/16
plasma cell	6/15
prostate	1/1
skin	12/24
soft tissue	7/9
thyroid	1/2
upper aerodigestive	11/22
urinary tract	15/29
uterus	5/5

Expression Distribution

WD40
PRP4

spliceosomal snRNP complex
U4/U6 snRNP
U4 snRNA binding
U6 snRNA binding
U4/U6 x U5 tri-snRNP complex
U2-type precatalytic spliceosome
Cajal body
spliceosomal complex
mRNA splicing, via spliceosome
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile
RNA splicing, via transesterification reactions
RNA splicing
nuclear speck
mRNA processing
mRNA metabolic process
RNA processing
RNA metabolic process
gene expression