Table of Contents

Phenformin 20μM R03 exp143

Phenformin 20μM R03 exp143

Mechanism of Action

Inhibits mitochondrial respiratory chain complex I, indirectly activates AMPK

Class / Subclass 1: Organelle Function / Mitochondrial Inhibitor
Class / Subclass 2: Metabolism / Metabolic Enzyme Activator

Technical Notes

Compound References

PubChem Name: Phenformin hydrochloride
Synonyms: Phenethylbiguanide hydrochloride
CAS #: 834-28-6
PubChem CID: 13266
IUPAC: 1-(diaminomethylidene)-2-(2-phenylethyl)guanidine;hydrochloride
INCHI Name: InChI=1S/C10H15N5.ClH/c11-9(12)15-10(13)14-7-6-8-4-2-1-3-5-8;/h1-5H,6-7H2,(H6,11,12,13,14,15);1H
INCHI Key: YSUCWSWKRIOILX-UHFFFAOYSA-N
Molecular Weight: 241.72
Canonical SMILES: C1=CC=C(C=C1)CCN=C(N)N=C(N)N.Cl
Isomeric SMILES: N/A
Molecular Formula: C10H16ClN5

Compound Supplier

Supplier Name: Sigma-Aldrich (Supelco)
Catalog #: P7045
Lot #: MKBV9127V

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C10H15N5 208.14493; found 208.14677

Dose Response Curve

Platform ID: PHENFORMINE
Min: -0.7796; Max: 41.1406

IC	Concentration (µM)
IC10	N/A
IC20	4.1494
IC30	21.8108
IC40	85.0051
IC50	N/A
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 03
Dose: 20µM
Days of incubation: 8
Doublings: 4.0
Numbers of reads: 11383615

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
2/4	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
GOT1	-4.58	<0.01
AGPAT6	-2.95	0.01
GCLC	-2.75	0.08
PGP	-2.70	0.08
PAFAH1B1	-2.59	0.41
SUCLG1	-2.46	0.32
ENO1	-2.40	0.32
SNCB	-2.34	0.32
RBM19	-2.30	0.32
PMPCA	-2.30	0.42
PLEKHA2	-2.27	0.38
XPO1	-2.24	0.84
PIPOX	-2.16	0.32
HNRNPA0	-2.14	0.32
GCDH	-2.12	0.32
C7orf55-LUC7L2	-2.09	0.41
C6orf132	-2.08	0.45
PFDN6	-2.07	0.41
NMT1	-2.06	0.41
TRAPPC3	-2.06	0.37
LRRC4C	-2.05	0.41
USHBP1	-2.04	0.37
APOA2	-2.04	0.37
CFL1	-2.04	0.82
ECHS1	-2.04	0.37
LETM2	-2.03	0.37
ADK	-2.02	0.37
NAE1	-2.01	0.42
ZNF470	-1.99	0.42
PURG	-1.99	0.42
METTL18	2.01	0.28
GARS	2.03	0.68
CYC1	2.03	0.26
ERBB2IP	2.04	0.26
SMC2	2.11	0.45
TMSB15B	2.12	0.54
SBDS	2.13	0.20
OR10H4	2.15	0.20
ITPK1	2.18	0.19
PCYT1A	2.19	0.19
UBA5	2.19	0.25
ENTPD6	2.19	0.25
ZNF804A	2.21	0.19
FAM50A	2.21	0.50
SMC1A	2.26	0.60
NEK1	2.27	0.20
TMEM261	2.27	0.17
NDUFAF7	2.28	0.17
KDSR	2.28	0.17
C1orf27	2.29	0.17
CD79A	2.33	0.25
SPC24	2.35	0.40
UQCC1	2.35	0.19
PRPS1	2.37	0.23
MTCH2	2.43	0.20
HSD17B12	2.59	0.17
PI4KB	2.68	0.03
MDH1	3.21	<0.01
ACSL4	3.34	<0.01
SLC25A51	4.98	<0.01

Screen	Correlation	Plot
QNZ 0.01μM R05 exp241	0.079	View
Phenformin 20μM R05 exp212	0.061	View
Rotenone 0.07μM R08 exp431	0.057	View
QNZ 0.01μM R08 exp439	0.056	View
NMS-873 0.07μM R07 exp380	0.051	View