Table of Contents

Etoposide 10μM R00 exp20

Mechanism of Action

Binds DNA-TopII complex, blocks re-ligation, induces strand breaks, semi-synthetic derivative of podophyllotoxin

Technical Notes

Protein References

  • PubChem Name: Etoposide
  • Synonyms: VP-16; VP-16-213
  • CAS #: 33419-42-0
  • PubChem CID: 36462
  • IUPAC: (5S,5aR,8aR,9R)-5-[[(2R,4aR,6R,7R,8R,8aS)-7,8-dihydroxy-2-methyl-4,4a,6,7,8,8a-hexahydropyrano[3,2-d][1,3]dioxin-6-yl]oxy]-9-(4-hydroxy-3,5-dimethoxyphenyl)-5a,6,8a,9-tetrahydro-5H-[2]benzofuro[6,5-f][1,3]benzodioxol-8-one
  • INCHI Name: InChI=1S/C29H32O13/c1-11-36-9-20-27(40-11)24(31)25(32)29(41-20)42-26-14-7-17-16(38-10-39-17)6-13(14)21(22-15(26)8-37-28(22)33)12-4-18(34-2)23(30)19(5-12)35-3/h4-7,11,15,20-22,24-27,29-32H,8-10H2,1-3H3/t11-,15+,20-,21-,22+,24-,25-,26-,27-,29+/m1/s1
  • INCHI Key: VJJPUSNTGOMMGY-MRVIYFEKSA-N
  • Molecular Weight: 588.6
  • Canonical SMILES: CC1OCC2C(O1)C(C(C(O2)OC3C4COC(=O)C4C(C5=CC6=C(C=C35)OCO6)C7=CC(=C(C(=C7)OC)O)OC)O)O
  • Isomeric SMILES: C[C@@H]1OC[C@@H]2[C@@H](O1)[C@@H]([C@H]([C@@H](O2)O[C@H]3[C@H]4COC(=O)[C@@H]4[C@@H](C5=CC6=C(C=C35)OCO6)C7=CC(=C(C(=C7)OC)O)OC)O)O
  • Molecular Formula: C29H32O13

Protein Supplier

  • Supplier Name: Sigma-Aldrich
  • Catalog #: E1383
  • Lot #: N/A

Protein Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C29H32O13 589.19157; found 589.19262

Dose Response Curve

  • Platform ID: Etoposide
  • Min: -33.3567; Max: 88.0314





IC Concentration (µM)
IC10 N/A
IC20 0.1877
IC30 0.2644
IC40 0.3501
IC50 0.4531
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
3/1ScoresViewViewViewViewView