Metformin 40μM R03 exp132

Mechanism of Action

Inhibits mitochondrial respiratory chain complex I, indirectly activates AMPK

  • Class / Subclass 1: Organelle Function / Mitochondrial Inhibitor
  • Class / Subclass 2: Signal Transduction / Kinase Activator

Technical Notes

Compound References

  • PubChem Name: Metformin hydrochloride
  • Synonyms: 1,1-Dimethylbiguanide hydrochloride
  • CAS #: 1115-70-4
  • PubChem CID: 14219
  • IUPAC: 3-(diaminomethylidene)-1,1-dimethylguanidine;hydrochloride
  • INCHI Name: InChI=1S/C4H11N5.ClH/c1-9(2)4(7)8-3(5)6;/h1-2H3,(H5,5,6,7,8);1H
  • INCHI Key: OETHQSJEHLVLGH-UHFFFAOYSA-N
  • Molecular Weight: 165.62
  • Canonical SMILES: CN(C)C(=N)N=C(N)N.Cl
  • Isomeric SMILES: N/A
  • Molecular Formula: C4H12ClN5

Compound Supplier

  • Supplier Name: Sigma-Aldrich
  • Catalog #: D150959
  • Lot #: BCBF1484V

Compound Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C4H11N5 130.10872; found 130.10841

Dose Response Curve

  • Platform ID: METFORMINE
  • Min: -12.1710; Max: 14.1584





IC Concentration (µM)
IC10 N/A
IC20 N/A
IC30 N/A
IC40 N/A
IC50 N/A
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

  • Round: 03
  • Dose: 40µM
  • Days of incubation: 8
  • Doublings: 7.9
  • Numbers of reads: 10584693

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
0/0ScoresViewViewViewViewView

  • Last modified: 2026/01/15 21:36
  • by 127.0.0.1