Campthothecin 0.001μM R06 exp270

Mechanism of Action

Inhibits topoisomerase I, stabilizes TopoI-DNA complex, causes DNA strand breaks

  • Class / Subclass 1: DNA Damage, Repair and Replication / Clastogen

Technical Notes

Compound References

  • PubChem Name: Camptothecin
  • Synonyms: Camptothecin; (S)-(+)-Camptothecin; CPT
  • CAS #: 7689-03-4
  • PubChem CID: 24360
  • IUPAC: (19S)-19-ethyl-19-hydroxy-17-oxa-3,13-diazapentacyclo[11.8.0.02,11.04,9.015,20]henicosa-1(21),2,4,6,8,10,15(20)-heptaene-14,18-dione
  • INCHI Name: InChI=1S/C20H16N2O4/c1-2-20(25)14-8-16-17-12(7-11-5-3-4-6-15(11)21-17)9-22(16)18(23)13(14)10-26-19(20)24/h3-8,25H,2,9-10H2,1H3/t20-/m0/s1
  • INCHI Key: VSJKWCGYPAHWDS-FQEVSTJZSA-N
  • Molecular Weight: 348.4
  • Canonical SMILES: CCC1(C2=C(COC1=O)C(=O)N3CC4=CC5=CC=CC=C5N=C4C3=C2)O
  • Isomeric SMILES: CC[C@@]1(C2=C(COC1=O)C(=O)N3CC4=CC5=CC=CC=C5N=C4C3=C2)O
  • Molecular Formula: C20H16N2O4

Compound Supplier

  • Supplier Name: Sigma-Aldrich
  • Catalog #: C9911
  • Lot #: N/A

Compound Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C20H16N2O4 349.11828; found 349.11567

Dose Response Curve

  • Platform ID: Camptothecin
  • Min: 25.8061; Max: 99.6985





IC Concentration (µM)
IC10 N/A
IC20 N/A
IC30 0.0006
IC40 N/A
IC50 0.0010
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

  • Round: 06
  • Dose: 1nM
  • Days of incubation: 8
  • Doublings: 5.8
  • Numbers of reads: 8689434

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
7/0ScoresViewViewViewViewView

  • Last modified: 2026/01/15 21:36
  • by 127.0.0.1