Table of Contents

CCCP 0.1μM R00 exp10

CCCP 0.1μM R00 exp10

Mechanism of Action

Mitochondrial proton gradient uncoupler, disrupts ATP synthesis

Class / Subclass 1: Organelle Function / Mitochondrial Inhibitor

Technical Notes

Protein References

PubChem Name: (3-Chlorophenyl)hydrazonomalononitrile
Synonyms: Carbonyl cyanide 3-chlorophenylhydrazone; Carbonyl Cyanide m-Chlorophenylhydrazone; Carbonyl cyanide 3-chlorophenylhydrazone;Carbonyl Cyanide m-Chlorophenylhydrazone
CAS #: 555-60-2
PubChem CID: 2603
IUPAC: 2-[(3-chlorophenyl)hydrazinylidene]propanedinitrile
INCHI Name: InChI=1S/C9H5ClN4/c10-7-2-1-3-8(4-7)13-14-9(5-11)6-12/h1-4,13H
INCHI Key: UGTJLJZQQFGTJD-UHFFFAOYSA-N
Molecular Weight: 204.61
Canonical SMILES: C1=CC(=CC(=C1)Cl)NN=C(C#N)C#N
Isomeric SMILES: N/A
Molecular Formula: C9H5ClN4

Protein Supplier

Supplier Name: Sigma-Aldrich
Catalog #: C2759
Lot #: MKBT2015V

Protein Characterization

HRMS (ESI-TOF) m/z: [M-H]- Calcd for C9H5NClN4 203.0130; found 202.9946

Dose Response Curve

Platform ID: CCCP
Min: -13.9463; Max: 78.4067

IC	Concentration (µM)
IC10	N/A
IC20	3.0761
IC30	4.2919
IC40	5.6392
IC50	7.2449
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 00
Dose: 100nM
Days of incubation: 8
Doublings: 6.7
Numbers of reads: 11956535

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
2/0	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
ADK	-2.95	0.05
PRAMEF25	-2.87	0.61
PRAMEF26	-2.85	0.61
CAMLG	-2.72	0.05
PADI2	-2.41	0.35
MOCS3	-2.40	0.35
MPHOSPH6	-2.26	0.37
RAB24	-2.20	0.35
EEF1E1	-2.19	0.35
RREB1	-2.17	0.35
TMEM240	-2.16	0.35
LAMTOR4	-2.11	0.61
DDX55	-2.07	0.61
KIAA1432	-2.06	0.50
SHOX2	-2.02	0.61
RPE	-2.02	0.61
UIMC1	-2.01	0.61
MAP1B	-2.01	0.61
TAF1A	-2.00	0.61
SEPHS2	-1.98	0.61
MYO9B	-1.97	0.61
PRAMEF9	-1.95	0.61
FCAR	-1.94	0.61
GMPPB	-1.94	0.61
RARS	-1.91	0.75
CEP57	-1.91	0.61
ACAN	-1.91	0.61
CSNK1D	-1.91	0.61
PRDM10	-1.89	0.61
LIAS	-1.89	0.61
C5orf28	2.07	0.31
PPP1R15B	2.10	0.33
FAM184A	2.11	0.33
FBXL19	2.12	0.44
RPL10A	2.14	0.38
PSMD13	2.17	0.35
SPZ1	2.19	0.31
ORC1	2.21	0.33
APEX1	2.22	0.16
NTNG1	2.23	0.16
RPS14	2.25	0.35
CCDC90B	2.27	0.14
GPSM3	2.27	0.33
PLG	2.28	0.14
NOP2	2.28	0.35
TRIM59	2.29	0.14
CDH17	2.31	0.14
MSLN	2.33	0.31
CKAP5	2.35	0.44
PRPF8	2.38	0.44
PPWD1	2.38	0.35
NDC1	2.40	0.12
SLCO1C1	2.43	0.12
EMD	2.47	0.12
USP27X	2.52	0.45
EGR2	2.58	0.26
IDE	2.61	0.11
ANKRD50	2.69	0.11
CHAF1A	3.14	0.14
COPE	3.22	0.12

Screen	Correlation	Plot
Chloramphenicol 2μM R00 exp12	0.081	View
Cycloheximide 0.2μM R00 exp15	0.074	View
Rifampicin 1μM R00 exp31	0.054	View
Alpha-Amanitin 0.5μM R00 exp5	0.057	View