Table of Contents

Tunicamycin 0.04μM R07 exp418

Mechanism of Action

Inhibits GlcNAc phosphotransferase (GPT), blocks N-linked glycoslylation, induces protein unfolding in ER, nucleoside antibiotic

Technical Notes

Protein References

  • PubChem Name: Tunicamycin from Streptomyces sp.
  • Synonyms: N/A
  • CAS #: 11089-65-9
  • PubChem CID: 16220051
  • IUPAC: (E)-N-[(2S,3R,4R,5R,6R)-2-[(2S,3S,4S,5R,6S)-3-acetamido-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[(2R)-2-[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]-2-hydroxyethyl]-4,5-dihydroxyoxan-3-yl]-12-methyltridec-2-enamide
  • INCHI Name: InChI=1S/C37H60N4O16/c1-18(2)12-10-8-6-4-5-7-9-11-13-23(45)39-26-30(50)27(47)21(54-36(26)57-35-25(38-19(3)43)29(49)28(48)22(17-42)55-35)16-20(44)33-31(51)32(52)34(56-33)41-15-14-24(46)40-37(41)53/h11,13-15,18,20-22,25-36,42,44,47-52H,4-10,12,16-17H2,1-3H3,(H,38,43)(H,39,45)(H,40,46,53)/b13-11+/t20-,21-,22+,25+,26-,27+,28+,29+,30-,31+,32-,33-,34-,35+,36+/m1/s1
  • INCHI Key: YJQCOFNZVFGCAF-WPTOCQRYSA-N
  • Molecular Weight: 816.9
  • Canonical SMILES: CC(C)CCCCCCCCC=CC(=O)NC1C(C(C(OC1OC2C(C(C(C(O2)CO)O)O)NC(=O)C)CC(C3C(C(C(O3)N4C=CC(=O)NC4=O)O)O)O)O)O
  • Isomeric SMILES: CC(C)CCCCCCCC/C=C/C(=O)N[C@@H]1[C@H]([C@H]([C@H](O[C@H]1O[C@H]2[C@H]([C@@H]([C@H]([C@@H](O2)CO)O)O)NC(=O)C)C[C@H]([C@@H]3[C@H]([C@H]([C@@H](O3)N4C=CC(=O)NC4=O)O)O)O)O)O
  • Molecular Formula: C37H60N4O16

Protein Supplier

  • Supplier Name: Sigma-Aldrich
  • Catalog #: T7765
  • Lot #: 037M4047V

Protein Characterization

  • HRMS (ESI-TOF) m/z: (M+NH4)+ Calcd for C37H60N4O16 834.43426; found 834.4337

Dose Response Curve

  • Platform ID: Tunicamycin
  • Min: -6.5253; Max: 80.8037





IC Concentration (µM)
IC10 N/A
IC20 0.0211
IC30 0.0328
IC40 0.0492
IC50 0.0748
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
4/15ScoresViewViewViewViewView