Table of Contents

Torin1 0.08μM R02 exp82

Torin1 0.08μM R02 exp82

Mechanism of Action

Inhibits mTORC1 and mTORC2, ATP competitive

Class / Subclass 1: Proteostasis / mTOR inhibitor
Class / Subclass 2: Signal Transduction / Kinase Inhibitor

Technical Notes

Protein References

PubChem Name: Torin 1
Synonyms: N/A
CAS #: 1222998-36-8
PubChem CID: 49836027
IUPAC: 1-[4-(4-propanoylpiperazin-1-yl)-3-(trifluoromethyl)phenyl]-9-quinolin-3-ylbenzo[h][1,6]naphthyridin-2-one
INCHI Name: InChI=1S/C35H28F3N5O2/c1-2-32(44)42-15-13-41(14-16-42)31-11-9-26(19-28(31)35(36,37)38)43-33(45)12-8-24-20-40-30-10-7-22(18-27(30)34(24)43)25-17-23-5-3-4-6-29(23)39-21-25/h3-12,17-21H,2,13-16H2,1H3
INCHI Key: AKCRNFFTGXBONI-UHFFFAOYSA-N
Molecular Weight: 607.6
Canonical SMILES: CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F
Isomeric SMILES: N/A
Molecular Formula: C35H28F3N5O2

Protein Supplier

Supplier Name: Selleck Chemicals
Catalog #: S2827
Lot #: N/A

Protein Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C35H28F3N5O2 608.22679; found 608.22884

Dose Response Curve

Platform ID: Torin1
Min: -11.3340; Max: 60.4301

IC	Concentration (µM)
IC10	N/A
IC20	0.0460
IC30	0.0897
IC40	0.1551
IC50	0.2563
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 02
Dose: 80nM
Days of incubation: 8
Doublings: 3.7
Numbers of reads: 11967482

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
0/9	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
UMPS	-3.24	0.33
THOC7	-2.62	0.11
TUBB	-2.62	0.22
UXS1	-2.60	0.11
CDK6	-2.49	0.13
MSH5	-2.43	0.22
SAFB	-2.31	0.38
PAG1	-2.29	0.22
RRAGA	-2.25	0.38
DROSHA	-2.20	0.39
LAMTOR4	-2.12	0.38
RHEB	-2.10	0.38
PACSIN2	-2.08	0.38
ZFP91	-2.07	0.38
TFAP2E	-2.07	0.39
ADAM17	-2.06	0.39
MCM5	-2.06	0.48
SLC9A3R1	-2.06	0.38
NRAS	-2.05	0.39
SCAPER	-2.05	0.39
SENP1	-2.04	0.38
PSMA1	-2.04	0.65
RSRC2	-2.03	0.83
GTF2E2	-2.03	0.39
U2AF2	-2.02	0.50
ACTL6A	-2.01	0.65
NPIPA5	-1.99	0.65
SCNN1G	-1.97	0.39
CDC45	-1.96	0.83
ACTB	-1.96	0.50
DEFB135	2.10	0.24
CMAS	2.11	0.20
KDM8	2.12	0.34
MTHFD1	2.12	0.49
COA6	2.13	0.18
MDH2	2.14	0.22
MRPS18A	2.14	0.22
PRKACA	2.18	0.15
EIF4A1	2.22	0.20
ATIC	2.22	0.20
MEMO1	2.22	0.13
NDUFAF3	2.25	0.24
WBSCR16	2.34	0.21
ATP5F1	2.37	0.12
TRMT6	2.45	0.08
KIAA0195	2.46	0.08
PTPMT1	2.48	0.22
GSK3A	2.49	0.04
GSK3B	2.53	0.04
ARID4B	2.55	0.06
BTK	2.57	0.06
IBA57	2.59	0.03
MRPL28	2.69	0.07
FIBP	2.78	0.03
METTL17	2.85	0.03
PPP3R1	2.85	0.01
PPP3CA	2.92	<0.01
RPL36A-HNRNPH2	2.94	0.45
PGM3	3.09	<0.01
EIF4EBP1	3.51	<0.01

Screen	Correlation	Plot
iCRT14 30μM R08 exp492	0.054	View
INK128 0.2μM R02 exp70	0.116	View
KU-0063794 3.8μM R02 exp71	0.071	View
Rapamycin 0.001μM R08 exp429	0.063	View
Rapamycin 2μM R07 exp306	0.062	View
Rapamycin 10μM R00 exp30	0.056	View
Rapamycin 1μM R00 exp29	0.055	View
Erlotinib 10μM R05 exp216	0.054	View