TCOF1

Entrez Summary: This gene encodes a nucleolar protein with a LIS1 homology domain. The protein is involved in ribosomal DNA gene transcription through its interaction with upstream binding factor (UBF). Mutations in this gene have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008].
UniProt Summary: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:12777385, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). {ECO:0000269|PubMed:12777385, ECO:0000269|PubMed:26399832}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Quinacrine 2.5μM R03 exp146	-2.38
Tozasertib 0.1μM R07 exp414	-2.24
2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312	-2.05
Rapamycin 2μM plus FK-506 5μM R07 exp308	-2
Radicicol 0.16μM R05 exp242	-2
Ibrutinib 1μM R07 exp372	-1.99
Pomalidomide 20μM R07 exp391	-1.97
Resminostat 0.5μM R07 exp393	-1.96
2-Methoxyestradiol 0.55μM R07 exp310	-1.95
R-DABN 8μM R03 exp111	-1.89
NFN1 1μM R03 exp100	-1.86
Nifurtimox 1μM R03 exp141	-1.84
TRAIL 50ng/ml R00 exp36	-1.84
Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307	-1.77
Cidofovir 10μM R07 exp345	-1.71
Nicotinamide Riboside 100μM R03 exp139	-1.71
Dorsomorphin 5μM R07 exp357	-1.7
Ammonium tetrathiomolybdate 10μM R08 exp448	1.77

Global Fraction of Cell Lines Where Essential: 59/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	2/28
blood	2/28
bone	0/26
breast	4/33
central nervous system	3/56
cervix	0/4
colorectal	1/17
esophagus	1/13
fibroblast	0/1
gastric	0/16
kidney	4/21
liver	0/20
lung	3/75
lymphocyte	0/16
ovary	6/26
pancreas	1/24
peripheral nervous system	1/16
plasma cell	0/15
prostate	0/1
skin	3/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	4/22
urinary tract	3/29
uterus	0/5

Expression Distribution

nucleolar large rRNA transcription by RNA polymerase I
RNA polymerase I core binding
neural crest formation
rRNA transcription
transcription by RNA polymerase I
transporter activity
scaffold protein binding
epithelial to mesenchymal transition
neural crest cell development
mesenchymal cell development
stem cell development
neural crest cell differentiation
ncRNA transcription
fibrillar center
mesenchymal cell differentiation
stem cell differentiation
mesenchyme development
regulation of translation
regulation of cellular amide metabolic process
protein heterodimerization activity
skeletal system development
posttranscriptional regulation of gene expression
chordate embryonic development
transcription, DNA-templated
nucleic acid-templated transcription
embryo development ending in birth or egg hatching
RNA biosynthetic process
nucleolus
anatomical structure formation involved in morphogenesis
embryo development
nucleobase-containing compound biosynthetic process
heterocycle biosynthetic process
aromatic compound biosynthetic process
organic cyclic compound biosynthetic process
RNA binding
cellular nitrogen compound biosynthetic process
cell development
RNA metabolic process
cellular macromolecule biosynthetic process
tissue development