WEE1

Entrez Summary: N/A
UniProt Summary: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
MK-1775 0.32μM R02 exp75	-3.31
Azithromycin 100μM R08 exp452	-2.67
Hydroxyurea 100μM R07 exp329	-2.34
NN-Dimethylsphingosine 2.5μM R08 exp508	-2.1
Pterostilbene 16μM R02 exp78	-2.09
6-Thio-2-deoxyguanosine 2μM R06 exp259	-2.07
Lead acetate 2000μM R08 exp498 no dilution day6	-1.93
Atovaquone 15μM R08 exp451	-1.88
Aminopterin 0.005μM R07 exp335	-1.87
Thapsigargin 0.005μM R07 exp407	-1.83
Torin1 0.08μM R02 exp82	-1.72
Staurosporine 0.02μM R08 exp524	-1.71
Ethambutol 25μM R08 exp481	1.71
NFN1 1μM R03 exp100	1.72
Rifampicin 10μM R00 exp32	1.72
Doxorubicin 0.02μM R08 exp478	1.73
Cisplatin 0.35μM R06 exp273	1.78
L-74142 5μM R03 exp131	1.8
NFN1 0.4μM R03 exp99	1.8
Nutlin-3A 1.6μM R06 exp294	1.81
Dexamethasone 0.006μM R07 exp351	1.83
Ibrutinib 1μM R07 exp372	1.84
Betulinic acid 10 to 15μM on day4 R05 exp222	1.87
2-Methoxyestradiol 0.55μM R07 exp310	1.9
Taxol 0.004μM R03 exp103	1.97
Vinorelbine 0.001μM R05 exp249	2.08
DABN 2μM R00 exp16	2.15
Chloramphenicol 2μM R00 exp12	2.19
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	2.21
Isoniazid 100μM R08 exp494	2.22
CVT-10216 0.1μM R06 exp278	2.54
Rapamycin 1μM R00 exp29	2.79
5-Fluorouracil 2μM R00 exp1	2.97
CCCP 1μM R00 exp11	3.72
Etoposide 0.1μM R05 exp198	4.63

Global Fraction of Cell Lines Where Essential: 739/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	26/26
breast	33/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	21/21
liver	20/20
lung	75/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Expression Distribution

Pkinase Tyr
Pkinase

non-membrane spanning protein tyrosine kinase activity
kinase activity
protein tyrosine kinase activity
negative regulation of G1/S transition of mitotic cell cycle
negative regulation of cell cycle G1/S phase transition
establishment of cell polarity
G2/M transition of mitotic cell cycle
cell cycle G2/M phase transition
peptidyl-tyrosine phosphorylation
peptidyl-tyrosine modification
regulation of G1/S transition of mitotic cell cycle
mitotic cell cycle checkpoint
regulation of cell cycle G1/S phase transition
cell cycle checkpoint
establishment or maintenance of cell polarity
negative regulation of mitotic cell cycle phase transition
magnesium ion binding
negative regulation of cell cycle phase transition
protein kinase activity
mitotic cell cycle phase transition
cell cycle phase transition
negative regulation of mitotic cell cycle
negative regulation of cell cycle process
regulation of mitotic cell cycle phase transition
regulation of cell cycle phase transition
microtubule cytoskeleton organization
neuron projection morphogenesis
plasma membrane bounded cell projection morphogenesis
cell projection morphogenesis
cell division
cell part morphogenesis
negative regulation of cell cycle
mitotic cell cycle process
regulation of mitotic cell cycle
neuron projection development
microtubule-based process
mitotic cell cycle
cell morphogenesis
regulation of cell cycle process
neuron development