Table of Contents

Trientine 500μM R08 exp534

Trientine 500μM R08 exp534

Mechanism of Action

Copper-selective chelator, enhances renal excretion of copper

Class / Subclass 1: Environmental Stresses / Metal Chelator

Technical Notes

Compound References

PubChem Name: Triethylenetetramine
Synonyms: N/A
CAS #: 112-24-3
PubChem CID: 5565
IUPAC: N'-[2-(2-aminoethylamino)ethyl]ethane-1,2-diamine
INCHI Name: InChI=1S/C6H18N4/c7-1-3-9-5-6-10-4-2-8/h9-10H,1-8H2
INCHI Key: VILCJCGEZXAXTO-UHFFFAOYSA-N
Molecular Weight: 146.23
Canonical SMILES: C(CNCCNCCN)N
Isomeric SMILES: N/A
Molecular Formula: QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA

Compound Supplier

Supplier Name: Combi-Blocks
Catalog #: QF-3275
Lot #: A32852

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C6H18N4 147.16042; found 147.1617

Dose Response Curve

Platform ID: Trientine
Min: 15.8115; Max: 99.8213

IC	Concentration (µM)
IC10	3609.0000
IC20	4737.0000
IC30	5675.0000
IC40	6582.0000
IC50	7540.0000
IC60	8639.0000
IC70	10020.0000
IC80	12000.0000
IC90	15750.0000

Screen Summary

Round: 08
Dose: 500µM
Days of incubation: 8
Doublings: 5.1
Numbers of reads: 15130273

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
3/4	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
VPS25	-3.60	0.15
GOT1	-3.33	<0.01
ODC1	-2.99	<0.01
RIC8A	-2.68	0.04
MDM2	-2.62	0.23
DPAGT1	-2.34	0.37
DEFB128	-2.32	0.45
NOP16	-2.16	0.58
PFN1	-2.15	0.42
GOLGA8H	-2.13	0.63
CSK	-2.12	0.42
PGGT1B	-2.11	0.42
MNT	-2.11	0.43
MTF2	-2.10	0.43
LAS1L	-2.10	0.63
NUBP1	-2.10	0.51
LCE2A	-2.06	0.45
RPF1	-2.05	0.43
PROSC	-2.05	0.43
PLEC	-2.05	0.43
RPS28	-2.04	0.63
NSMCE1	-2.03	0.43
RGS17	-2.03	0.43
BEX4	-2.01	0.43
ZBTB16	-2.00	0.45
CLNS1A	-1.99	0.43
ZNF507	-1.99	0.45
SPATA12	-1.96	0.45
RNF121	-1.95	0.51
CCL2	-1.95	0.51
NLK	2.14	0.27
MAP6	2.14	0.19
DAZAP1	2.14	0.15
ZBTB7A	2.15	0.15
PRM3	2.15	0.23
FPR2	2.15	0.19
COQ7	2.20	0.12
TRIM41	2.21	0.17
BEND3	2.21	0.12
TARS2	2.22	0.17
DNM1L	2.22	0.45
C2orf69	2.27	0.10
NDUFA2	2.28	0.15
GCNT4	2.29	0.10
GRSF1	2.34	0.12
MPHOSPH10	2.35	0.21
ATP5SL	2.39	0.08
ZNF544	2.40	0.08
NDUFB9	2.41	0.10
EIF3H	2.42	0.10
NDUFC1	2.45	0.07
ROBO1	2.45	0.19
LEO1	2.50	0.06
NDUFAF7	2.50	0.06
GNA11	2.51	0.08
NPRL3	2.68	0.08
LARS2	2.82	0.04
ASUN	2.85	0.01
ATP7A	4.91	<0.01
ATOX1	5.56	<0.01

GO Term	Fold Change	Genes
mitochondrial respiratory chain complex I assembly	53.11	NDUFAF7,NDUFC1,NDUFB9,DMAC2,NDUFA2
NADH dehydrogenase complex assembly	53.11	NDUFAF7,NDUFC1,NDUFB9,DMAC2,NDUFA2
mitochondrial respiratory chain complex assembly	31.74	NDUFAF7,NDUFC1,NDUFB9,DMAC2,NDUFA2
mitochondrion organization	10.52	ATP7A,NDUFAF7,NDUFC1,NDUFB9,DMAC2,NDUFA2,DNM1L,TP53