Table of Contents

Rotenone 20μM R00 exp34

Mechanism of Action

Inhibits complex I, blocks electron transfer from iron-sulfur centers to ubiquinone, generates ROS, also inhibits microtubule assembly

Technical Notes

Protein References

  • PubChem Name: Rotenone
  • Synonyms: N/A
  • CAS #: 83-79-4
  • PubChem CID: 6758
  • IUPAC: (1S,6R,13S)-16,17-dimethoxy-6-prop-1-en-2-yl-2,7,20-trioxapentacyclo[11.8.0.03,11.04,8.014,19]henicosa-3(11),4(8),9,14,16,18-hexaen-12-one
  • INCHI Name: InChI=1S/C23H22O6/c1-11(2)16-8-14-15(28-16)6-5-12-22(24)21-13-7-18(25-3)19(26-4)9-17(13)27-10-20(21)29-23(12)14/h5-7,9,16,20-21H,1,8,10H2,2-4H3/t16-,20-,21+/m1/s1
  • INCHI Key: JUVIOZPCNVVQFO-HBGVWJBISA-N
  • Molecular Weight: 394.4
  • Canonical SMILES: CC(=C)C1CC2=C(O1)C=CC3=C2OC4COC5=CC(=C(C=C5C4C3=O)OC)OC
  • Isomeric SMILES: CC(=C)[C@H]1CC2=C(O1)C=CC3=C2O[C@@H]4COC5=CC(=C(C=C5[C@@H]4C3=O)OC)OC
  • Molecular Formula: C23H22O6

Protein Supplier

  • Supplier Name: Sigma-Aldrich
  • Catalog #: R8875
  • Lot #: SLBG7568V

Protein Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C23H22O6 395.14891; found 395.15075

Dose Response Curve

  • Platform ID: Rotenone
  • Min: 11.0154; Max: 87.2842





IC Concentration (µM)
IC10 N/A
IC20 0.0123
IC30 0.0322
IC40 0.0711
IC50 0.1468
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
15/52ScoresViewViewViewViewView