Disulfiram 4.3μM R06 exp281 [The Human Chemical-Genetic Interaction Map Project]

ALDH1 inhibitor, putative 26S proteasome inhibitor

Class / Subclass 1: Metabolism / Metabolic Enzyme Inhibitor
Class / Subclass 2: Proteostasis / Ubiquitin-Proteasome Inhibitor

Compound References

PubChem Name: Disulfiram
Synonyms: Tetraethylthiuram disulfide; TETD
CAS #: 97-77-8
PubChem CID: 3117
IUPAC: diethylcarbamothioylsulfanyl N,N-diethylcarbamodithioate
INCHI Name: InChI=1S/C10H20N2S4/c1-5-11(6-2)9(13)15-16-10(14)12(7-3)8-4/h5-8H2,1-4H3
INCHI Key: AUZONCFQVSMFAP-UHFFFAOYSA-N
Molecular Weight: 296.5
Canonical SMILES: CCN(CC)C(=S)SSC(=S)N(CC)CC
Isomeric SMILES: N/A
Molecular Formula: C10H20N2S4

Compound Supplier

Supplier Name: Sigma-Aldrich (Supelco)
Catalog #: PHR1690
Lot #: LRAA6757

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C10H20N2S4 297.05821; found 297.05867

Dose Response Curve

Platform ID: Disulfiram
Min: -18.8948; Max: 99.3710

IC	Concentration (µM)
IC10	N/A
IC20	5.5369
IC30	5.7585
IC40	5.9467
IC50	6.1249
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 06
Dose: 4.3µM
Days of incubation: 8
Doublings: 5.8
Numbers of reads: 9578940

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
1/2	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
SLC30A1	-4.10	<0.01
TXNL4A	-3.25	0.46
ARIH1	-2.69	0.16
RPSA	-2.45	0.86
SEPHS1	-2.44	0.18
VPS25	-2.44	0.78
OTUD5	-2.39	0.35
ZNF407	-2.24	0.30
GCLM	-2.22	0.30
GCLC	-2.21	0.30
ABCA5	-2.19	0.30
UACA	-2.17	0.30
PLK2	-2.16	0.30
FLCN	-2.12	0.43
FBXO7	-2.10	0.35
PPP2R2A	-2.08	0.36
PRKAA1	-2.07	0.36
ANKRD32	-2.07	0.64
ZNF208	-2.04	0.40
MINA	-2.02	0.71
LTBR	-2.01	0.43
TMEM206	-1.98	0.45
CPA4	-1.95	0.46
AP3M2	-1.94	0.46
CSRP2BP	-1.94	0.46
ST20	-1.90	0.90
CDIPT	-1.89	0.90
STT3A	-1.89	0.86
OR52B4	-1.85	0.64
RFC3	-1.84	0.90
SUPT20HL2	1.95	0.88
RAN	1.95	0.88
CACTIN	1.98	0.65
LRRC63	1.98	0.88
HNRNPM	1.99	0.81
RHOC	2.00	0.81
PES1	2.01	0.88
RRM2	2.05	0.88
DNM2	2.09	0.88
PNKD	2.09	0.65
INO80C	2.09	0.50
CHST12	2.10	0.65
IMP3	2.11	0.88
ABLIM1	2.19	0.35
NPM3	2.20	0.50
IL4I1	2.28	0.29
BAGE4	2.30	0.88
BAGE5	2.30	0.88
RPL37A	2.30	0.88
BAGE2	2.31	0.88
BAGE3	2.31	0.88
BAGE	2.31	0.88
NAA40	2.34	0.50
CSN3	2.38	0.20
TINAG	2.45	0.17
SETD1A	2.51	0.35
COPB2	2.56	0.72
POLA1	2.59	0.88
HOXD12	2.80	0.03
KMT2D	3.13	<0.01

Last modified: 2026/01/15 21:36
by 127.0.0.1