TNF-alpha 44ng/ml R08 exp533

Ligand for TNFR1, induces apoptosis or necrosis

Class / Subclass 1: Signal Transduction / Other Receptor Agonist

Compound References

PubChem Name: N/A
Synonyms: N/A
CAS #: N/A
PubChem CID: N/A
IUPAC: N/A
INCHI Name: N/A
INCHI Key: N/A
Molecular Weight: 16.9kDa
Canonical SMILES: N/A
Isomeric SMILES: N/A
Molecular Formula: MRKR+KPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN

Compound Supplier

Supplier Name: Cusabio
Catalog #: CSB-AP002151HU
Lot #: 03962

Compound Characterization

Characterization data not available.

Dose Response Curve

Platform ID: TNF_alpha
Min: -13.9154; Max: 15.1482

IC	Concentration (µM)
IC10	N/A
IC20	N/A
IC30	N/A
IC40	N/A
IC50	N/A
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 08
Dose: 44ng/mL
Days of incubation: 8
Doublings: 6.6
Numbers of reads: 14832845

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
8/0	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
TRAF2	-4.62	<0.01
TNFAIP3	-4.04	<0.01
RNF31	-3.76	<0.01
ATG9A	-3.60	<0.01
IKBKG	-3.40	<0.01
WIPI2	-3.19	<0.01
CFLAR	-2.94	<0.01
C12orf44	-2.78	<0.01
CCT2	-2.74	0.57
VPS25	-2.67	0.41
RIPK1	-2.43	0.06
ZNF30	-2.43	0.06
CNOT2	-2.41	0.06
BIRC2	-2.32	0.15
LUC7L2	-2.28	0.15
UBE2N	-2.28	0.11
NCOA5	-2.27	0.15
ZNF638	-2.24	0.14
DUSP11	-2.22	0.14
CNOT4	-2.20	0.15
C10orf142	-2.19	0.22
MAP3K7	-2.15	0.15
KIF6	-2.14	0.15
DCAF15	-2.07	0.32
HOXA5	-2.06	0.22
CD1D	-2.05	0.23
PIK3R3	-1.99	0.30
C2orf88	-1.98	0.31
PPHLN1	-1.98	0.41
FRG2B	-1.96	0.32
CDC5L	1.93	0.61
SUPT6H	1.96	0.68
TRIOBP	1.96	0.60
ANXA5	1.97	0.60
RPS27A	1.97	0.60
CASP8	1.98	0.60
BANF1	1.98	0.71
ATP6V0B	2.01	0.79
MDM2	2.01	0.66
BEND3	2.02	0.60
ZDHHC3	2.03	0.61
RPS29	2.06	0.61
PNO1	2.06	0.72
OR10X1	2.09	0.60
PAK4	2.10	0.46
POLR1A	2.11	0.61
ZNF431	2.11	0.61
KDM6A	2.13	0.43
NVL	2.19	0.70
CATSPER3	2.20	0.61
PAFAH1B1	2.30	0.72
CRAMP1L	2.31	0.20
FADD	2.32	0.20
POLE	2.41	0.61
YTHDC2	2.43	0.20
TSR2	2.45	0.70
CDC34	2.50	0.13
DDX3X	2.62	0.13
NUP88	2.65	0.13
PSMA3	2.75	0.15

GO Term	Fold Change	Genes
linear polyubiquitin binding	662.76	RNF31,MAP3K7
negative regulation of necroptotic process	132.55	RNF31,RIPK1,BIRC2
regulation of necroptotic process	101.96	RNF31,CFLAR,RIPK1,BIRC2
programmed necrotic cell death	101.96	TRAF2,ATG9A,RIPK1,BIRC2
regulation of programmed necrotic cell death	88.37	RNF31,CFLAR,RIPK1,BIRC2
K63-linked polyubiquitin modification-dependent protein binding	82.84	TNFAIP3,RNF31,IKBKG
ubiquitin-like protein binding	33.70	TNFAIP3,RNF31,IKBKG,BIRC2,UBE2N,MAP3K7
ubiquitin binding	33.47	TNFAIP3,IKBKG,BIRC2,UBE2N,MAP3K7
positive regulation of canonical NF-kappaB signal transduction	27.62	TRAF2,RNF31,IKBKG,CFLAR,RIPK1,BIRC2,UBE2N,MAP3K7
positive regulation of NF-kappaB transcription factor activity	27.05	TRAF2,RNF31,IKBKG,CFLAR,RIPK1,UBE2N
regulation of canonical NF-kappaB signal transduction	23.39	TRAF2,TNFAIP3,RNF31,IKBKG,CFLAR,RIPK1,BIRC2,UBE2N,MAP3K7
protein polyubiquitination	19.49	TRAF2,TNFAIP3,RNF31,BIRC2,UBE2N,DCAF15
positive regulation of DNA-binding transcription factor activity	16.78	TRAF2,RNF31,IKBKG,CFLAR,RIPK1,UBE2N
ubiquitin protein ligase binding	15.57	TRAF2,RNF31,IKBKG,CCT2,RIPK1,UBE2N,MAP3K7
ubiquitin-like protein ligase binding	14.68	TRAF2,RNF31,IKBKG,CCT2,RIPK1,UBE2N,MAP3K7
regulation of DNA-binding transcription factor activity	12.40	TRAF2,TNFAIP3,RNF31,IKBKG,CFLAR,RIPK1,UBE2N
protein ubiquitination	7.76	TRAF2,TNFAIP3,RNF31,BIRC2,UBE2N,CNOT4,DCAF15
positive regulation of immune response	7.56	TRAF2,TNFAIP3,RNF31,IKBKG,UBE2N,MAP3K7,CD1D
intracellular protein-containing complex	6.82	TRAF2,RNF31,IKBKG,CCT2,CNOT2,UBE2N,CNOT4,MAP3K7,DCAF15

GO Term	Fold Change	Genes
TRAIL-activated apoptotic signaling pathway	276.15	FADD,ZDHHC3,CASP8
extrinsic apoptotic signaling pathway via death domain receptors	52.60	DDX3X,FADD,ZDHHC3,CASP8
ribosome biogenesis	11.35	NUP88,DDX3X,TSR2,NVL,PNO1,UTP3

TNF-alpha 44ng/ml R08 exp533

Mechanism of Action

Technical Notes

Compound References

Compound Supplier

Compound Characterization

Dose Response Curve

Screen Summary

Screen Results