Oligomycin-A 2μM R00 exp25

Oligomycin-A 2μM R00 exp25

Mechanism of Action

Inhibits F0 proton channel of mitochondrial ATP synthase, blocks electron flow through ETC

Class / Subclass 1: Organelle Function / Mitochondrial Inhibitor

Technical Notes

Protein References

PubChem Name: Oligomycin A
Synonyms: MCH 32
CAS #: 579-13-5
PubChem CID: 5281899
IUPAC: (1S,4E,5'R,6R,6'R,7S,8R,10S,11S,12R,14S,15R,16S,18E,20E,22S,25R,27S,28R,29S)-22-ethyl-7,11,14,15-tetrahydroxy-6'-[(2S)-2-hydroxypropyl]-5',6,8,10,12,14,16,28,29-nonamethylspiro[2,26-dioxabicyclo[23.3.1]nonacosa-4,18,20-triene-27,2'-oxane]-3,9,13-trione
INCHI Name: InChI=1S/C45H74O11/c1-12-34-17-15-13-14-16-27(4)42(51)44(11,53)43(52)32(9)40(50)31(8)39(49)30(7)38(48)26(3)18-21-37(47)54-41-29(6)35(20-19-34)55-45(33(41)10)23-22-25(2)36(56-45)24-28(5)46/h13-15,17-18,21,25-36,38,40-42,46,48,50-51,53H,12,16,19-20,22-24H2,1-11H3/b14-13+,17-15+,21-18+/t25-,26-,27+,28+,29+,30-,31-,32-,33-,34-,35-,36-,38+,40+,41+,42-,44+,45-/m1/s1
INCHI Key: MNULEGDCPYONBU-WMBHJXFZSA-N
Molecular Weight: 791.1
Canonical SMILES: CCC1CCC2C(C(C(C3(O2)CCC(C(O3)CC(C)O)C)C)OC(=O)C=CC(C(C(C(=O)C(C(C(C(=O)C(C(C(CC=CC=C1)C)O)(C)O)C)O)C)C)O)C)C
Isomeric SMILES: CC[C@H]\\1CC[C@@H]2[C@@H]([C@@H]([C@H]([C@@]3(O2)CC[C@H]([C@H](O3)C[C@H](C)O)C)C)OC(=O)/C=C/[C@H]([C@@H]([C@H](C(=O)[C@H]([C@@H]([C@H](C(=O)[C@@]([C@@H]([C@H](C/C=C/C=C1)C)O)(C)O)C)O)C)C)O)C)C
Molecular Formula: C45H74O11

Protein Supplier

Supplier Name: Tocris Bioscience
Catalog #: 4110
Lot #: 6A/200926

Protein Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C45H74O11 791.53039; found 791.53025

Dose Response Curve

Dose response curve not available.

Screen Summary

Round: 00
Dose: 2µM
Days of incubation: 8
Doublings: 4.1
Numbers of reads: 12775855

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
19/188	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
PARL	-4.71	<0.01
TCF4	-4.49	<0.01
PRKAG1	-4.12	<0.01
SOX4	-3.55	<0.01
SPG7	-3.34	<0.01
COX7A2L	-3.09	<0.01
UXS1	-3.04	<0.01
PSMA5	-2.97	0.04
PRKAA1	-2.81	<0.01
KCMF1	-2.77	<0.01
PSPC1	-2.75	<0.01
LRRC16A	-2.74	<0.01
PGP	-2.66	0.01
CDCA8	-2.59	0.04
DNAJC7	-2.59	0.04
SLC25A5	-2.55	0.02
PCBP2	-2.44	0.04
NME6	-2.36	0.05
MRS2	-2.36	0.05
SRF	-2.35	0.08
SOX7	-2.35	0.08
ELF2	-2.33	0.05
ZNF319	-2.32	0.05
ACACA	-2.32	0.09
DNAH17	-2.29	0.14
LDHB	-2.29	0.09
ARHGEF10	-2.29	0.06
WT1	-2.27	0.07
VPS53	-2.26	0.21
PFKP	-2.25	0.07
DDX3X	3.52	<0.01
NDUFAF6	3.53	<0.01
NDUFAF1	3.54	<0.01
PPP3R1	3.58	<0.01
TFB1M	3.58	<0.01
NDUFC2	3.60	<0.01
NDUFB10	3.62	<0.01
NDUFS2	3.63	<0.01
YLPM1	3.68	<0.01
TMEM261	3.78	<0.01
NDUFS5	3.80	<0.01
BRD1	3.80	<0.01
NDUFB8	3.80	<0.01
NDUFA3	3.82	<0.01
RTN4IP1	3.86	<0.01
C10orf137	3.87	<0.01
PET117	3.91	<0.01
GRSF1	3.98	<0.01
NDUFB11	3.99	<0.01
SLC25A51	4.24	<0.01
ACAD9	4.31	<0.01
TXNIP	4.37	<0.01
NDUFA11	4.42	<0.01
NDUFA2	4.50	<0.01
NDUFA1	5.25	<0.01
RBM26	5.30	<0.01
TIMM21	5.62	<0.01
CLUH	5.98	<0.01
STARD7	8.30	<0.01
OMA1	8.37	<0.01

Screen	Correlation	Plot
UM0125461 0.74μM R02 exp84	0.118	View
Thimerosal 0.85μM R08 exp529	0.1	View
iCRT14 30μM R08 exp492	0.095	View
Rapamycin 10μM R00 exp30	0.062	View
Ribavirin 10 to 15μM on day4 R04 exp160	0.055	View
Tubacin 1.6μM R07 exp416	0.054	View
Rapamycin 1μM R00 exp29	0.054	View
Vemurafenib 6.6μM R08 exp434	0.054	View
HMS-I2 10μM R06 exp288	0.124	View
Hydroxyurea 100μM R07 exp329	0.109	View
Artemisinin 50μM R08 exp450	0.097	View
Calcium Ionophore 0.4μM R07 exp342	0.095	View
Atovaquone 15μM R08 exp451	0.09	View
Genistein 15μM R07 exp360	0.088	View
Mubritinib 0.2μM R02 exp65	0.068	View
Chloramphenicol 20μM R00 exp13	0.065	View
LLY-283 2.6μM R06 exp290	0.06	View
Mubritinib 0.2μM R01 exp48	0.055	View
Hippuristanol 0.12μM R08 exp488	0.052	View

GO Term	Fold Change	Genes
mitochondrial respiratory chain complex I assembly	159.44	TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,NDUFB11,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6
NADH dehydrogenase complex assembly	159.44	TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,NDUFB11,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6
mitochondrial respiratory chain complex I	144.76	NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1
respiratory chain complex I	134.81	NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFC2,NDUFAF1
NADH dehydrogenase complex	134.81	NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2
mitochondrial electron transport, NADH to ubiquinone	130.91	NDUFA1,NDUFA2,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1
mitochondrial respiratory chain complex assembly	108.01	OMA1,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,NDUFB11,PET117,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6
proton motive force-driven ATP synthesis	92.12	NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2
respiratory chain complex	82.16	NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1
mitochondrial respirasome	81.07	NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1
respirasome	74.15	NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1
oxidative phosphorylation	67.00	NDUFA1,NDUFA2,NDUFA11,SLC25A51,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1
inner mitochondrial membrane protein complex	49.87	TIMM21,NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1
aerobic respiration	47.04	NDUFA1,NDUFA2,NDUFA11,SLC25A51,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1
mitochondrion organization	23.63	OMA1,CLUH,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,NDUFB11,PET117,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6
mitochondrial inner membrane	21.68	OMA1,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,SLC25A51,NDUFB11,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6,TIMMDC1
organelle inner membrane	19.47	OMA1,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,SLC25A51,NDUFB11,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6,TIMMDC1
mitochondrial membrane	15.66	OMA1,STARD7,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,SLC25A51,NDUFB11,RTN4IP1,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6,TIMMDC1
mitochondrial envelope	14.70	OMA1,STARD7,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,SLC25A51,NDUFB11,RTN4IP1,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6,TIMMDC1

Table of Contents

Oligomycin-A 2μM R00 exp25

Mechanism of Action

Technical Notes

Protein References

Protein Supplier

Protein Characterization

Dose Response Curve

Screen Summary

Screen Results