Nocodazole 0.2μM R02 exp64

Inhibits microtubule polymerization and mitotic spindle formation, induces spindle assembly checkpoint (SAC)

Class / Subclass 1: Cell Cycle / Microtubule Poison
Class / Subclass 2: Organelle Function / Cytoskeletal Inhibitor

Compound References

PubChem Name: Nocodazole
Synonyms: Oncodazole; R17934
CAS #: 31430-18-9
PubChem CID: 4122
IUPAC: methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate
INCHI Name: InChI=1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
INCHI Key: KYRVNWMVYQXFEU-UHFFFAOYSA-N
Molecular Weight: 301.32
Canonical SMILES: COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3
Isomeric SMILES: N/A
Molecular Formula: C14H11N3O3S

Compound Supplier

Supplier Name: Sigma-Aldrich
Catalog #: M1404
Lot #: N/A

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C14H11N3O3S 302.05939; found 302.05966

Dose Response Curve

Platform ID: Nocodazole
Min: -9.8754; Max: 86.3481

IC	Concentration (µM)
IC10	N/A
IC20	0.0313
IC30	0.0403
IC40	0.0496
IC50	0.0601
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 02
Dose: 200nM
Days of incubation: 8
Doublings: 0.0
Numbers of reads: 9840623

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
0/63	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
ESPL1	-2.83	0.33
ATP6V1A	-2.78	0.27
COPG1	-2.53	0.27
GOLGA8H	-2.45	0.67
HSPG2	-2.41	0.27
CNTRL	-2.39	0.27
SLC47A1	-2.38	0.27
CCDC27	-2.32	0.39
ZFAND5	-2.32	0.29
YLPM1	-2.31	0.39
PSMB3	-2.30	0.62
PAQR6	-2.29	0.27
UBE2E1	-2.28	0.27
LIG1	-2.28	0.67
WDR43	-2.21	0.61
EME1	-2.18	0.29
SKIV2L2	-2.16	0.61
OR51Q1	-2.13	0.41
LOC200726	-2.13	0.41
ECT2	-2.12	0.41
UBE4A	-2.12	0.33
ERCC8	-2.10	0.35
SART1	-2.10	0.76
KANSL2	-2.08	0.42
RPL30	-2.06	0.61
RFC1	-2.06	0.76
INO80E	-2.05	0.39
RNPC3	-2.04	0.42
DNM2	-2.03	0.76
PSAP	-2.02	0.42
ZDHHC4	2.77	<0.01
AP5Z1	2.82	<0.01
MPP6	2.87	<0.01
GMPPB	2.88	0.04
SUMF2	2.93	<0.01
HIBADH	2.97	0.00
ACTB	2.99	<0.01
TTYH3	3.10	<0.01
KBTBD2	3.14	0.00
ZNRF2	3.18	0.00
MYL7	3.23	0.00
HECW1	3.24	0.00
TNRC18	3.31	0.00
ZWILCH	3.34	0.00
PELO	3.35	<0.01
IGFBP1	3.46	0.00
BMPER	3.49	0.00
HOXA11	3.64	0.00
MAD2L1BP	3.79	0.00
AMZ1	3.96	0.00
MAD2L1	4.02	0.00
CDC20	4.33	<0.01
BUB1B	4.66	0.00
RBM14-RBM4	5.04	0.00
RBM14	5.42	0.00
MAD1L1	5.43	0.00
BUB3	5.78	0.00
KNTC1	6.55	0.00
TRIP13	6.63	0.00
ZW10	7.05	0.00

Screen	Correlation	Plot
SB743921 0.001μM R05 exp244	0.221	View
Rotenone 2μM R00 exp33	0.197	View
Vinblastine 0.015μM R05 exp208	0.19	View
R-DABN 8μM R03 exp111	0.18	View
Vinblastine 0.2μM R03 exp108	0.077	View
Nocodazole 0.1μM R03 exp94	0.217	View
Nocodazole 0.2μM R00 exp24	0.212	View
DABN racemic mixture R03 exp93	0.185	View
HMS-I1 10μM R06 exp256	0.184	View
BI-6727 0.04μM R03 exp98	0.1	View
DABN 20μM R00 exp17	0.078	View
BI-2536 0.02μM R03 exp96	0.054	View
GSK461364A 0.1μM R03 exp126	0.052	View

GO Term	Fold Change	Genes
mitotic spindle assembly checkpoint signaling	177.69	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH
spindle assembly checkpoint signaling	177.69	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH
mitotic spindle checkpoint signaling	177.69	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH
negative regulation of mitotic sister chromatid segregation	160.49	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH
negative regulation of mitotic sister chromatid separation	160.49	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH
spindle checkpoint signaling	152.50	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,ZWILCH
negative regulation of chromosome segregation	150.76	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH
negative regulation of chromosome separation	150.76	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH
negative regulation of metaphase/anaphase transition of cell cycle	150.76	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH
negative regulation of mitotic metaphase/anaphase transition	142.66	ZW10,TRIP13,KNTC1,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH
negative regulation of sister chromatid segregation	142.66	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,ZWILCH
negative regulation of nuclear division	108.16	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH
regulation of mitotic sister chromatid segregation	93.87	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,MAD2L1,MAD2L1BP,ZWILCH
regulation of mitotic sister chromatid separation	85.78	ZW10,TRIP13,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH
regulation of chromosome separation	75.73	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH
regulation of mitotic metaphase/anaphase transition	68.32	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH,ACTB
regulation of metaphase/anaphase transition of cell cycle	66.10	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH,ACTB
regulation of sister chromatid segregation	59.04	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH,ACTB
regulation of chromosome segregation	48.26	ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH,ACTB

Nocodazole 0.2μM R02 exp64

Mechanism of Action

Technical Notes

Compound References

Compound Supplier

Compound Characterization

Dose Response Curve

Screen Summary

Screen Results