CTU1

Entrez Summary: N/A
UniProt Summary: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. {ECO:0000255|HAMAP-Rule:MF_03053, ECO:0000269|PubMed:19017811}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Erlotinib 10μM R05 exp216	-2.4
GSK2606414 1μM R05 exp236	-2.16
Colchicine 0.009μM R05 exp215	-1.85
Epigallocatechin gallate 20μM R05 exp230	-1.8
1-Methyl-nicotinamide-chloride 1000μM R03 exp113	-1.73
GSK-J4 1-1.25μM to day4 R07 exp363	1.7
Trimetrexate 0.03μM R08 exp535	1.7
Lead acetate 2000μM R08 exp498 no dilution day6	1.76
CoCl2 18μM R07 exp346	1.8
Hippuristanol 0.12μM R08 exp489 no dilution day6	1.84
UM0129480 7μM R02 exp85	1.85
QNZ 0.01μM R08 exp439	1.92
CR131-b 0.005μM R08 exp474	1.93
Nocodazole 0.2μM R02 exp64	1.93
Thapsigargin 0.005μM R07 exp407	2.01
Rotenone 0.07μM R08 exp431	2.09
NMS-873 0.07μM R07 exp380	2.12
Mubritinib 0.2μM R02 exp65	2.23
Hippuristanol 0.12μM R08 exp488	2.33
Artemisinin 50μM R08 exp450	2.35
2-Deoxy-D-glucose 800μM R05 exp214	2.58
MS023 7μM R03 exp135	2.69
6-Thio-2-deoxyguanosine 2μM R06 exp259	2.76
MLN-4924 0.2μM R00 exp21	3
Mubritinib 0.2μM R01 exp48	3.22

Global Fraction of Cell Lines Where Essential: 6/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	1/28
bone	1/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	1/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	1/5

Expression Distribution

cytosolic tRNA wobble base thiouridylase complex
protein urmylation
tRNA wobble position uridine thiolation
tRNA thio-modification
nucleotidyltransferase activity
tRNA wobble uridine modification
tRNA wobble base modification
tRNA binding
tRNA modification
tRNA processing
RNA modification
tRNA metabolic process
ncRNA processing
ncRNA metabolic process
protein modification by small protein conjugation
RNA processing
protein modification by small protein conjugation or removal
RNA metabolic process
gene expression