IWS1

Entrez Summary: N/A
UniProt Summary: Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. {ECO:0000269|PubMed:17184735, ECO:0000269|PubMed:17234882, ECO:0000269|PubMed:19141475}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
ABT-702 5μM plus CoCl2 18μM R07 exp320	-2.36
FK-506 5μM R07 exp358	-2.32
A-769662 20μM R07 exp331	-2.21
Latrunculin-B 10μM R07 exp374	-1.94
Asunaprenir 3μM R07 exp336	-1.89
Cycloheximide 0.02μM R00 exp14	-1.84
CoCl2 18μM R07 exp346	-1.81
Dexamethasone 0.006μM R07 exp351	-1.79
Olaparib 4μM R08 exp512	-1.72
UM0011500 5μM R05 exp245	-1.7
GSK461364A 0.1μM R03 exp126	1.7
Clomiphene 4.4μM R02 exp68	1.71
KU-0063794 3.8μM R02 exp71	1.72
MS023 2μM R03 exp134	1.73
Deguelin 0.05μM R02 exp69	1.74
Hippuristanol 0.12μM R08 exp488	1.74
A-395 10μM R05 exp218	1.78
Ethanol 0.01 R05 exp234	1.82
Hippuristanol 0.12μM R08 exp489 no dilution day6	2.27

Global Fraction of Cell Lines Where Essential: 1/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	1/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

regulation of histone H3-K36 trimethylation
regulation of mRNA export from nucleus
regulation of histone H3-K36 methylation
regulation of ribonucleoprotein complex localization
regulation of histone H4 acetylation
regulation of RNA export from nucleus
regulation of nucleobase-containing compound transport
regulation of protein export from nucleus
regulation of histone acetylation
regulation of peptidyl-lysine acetylation
regulation of histone methylation
regulation of protein acetylation
transcription elongation from RNA polymerase II promoter
DNA-templated transcription, elongation
regulation of nucleocytoplasmic transport
regulation of mRNA processing
regulation of histone modification
mRNA transport
regulation of chromatin organization
nucleic acid transport
RNA transport
establishment of RNA localization
RNA localization
regulation of intracellular protein transport
nucleobase-containing compound transport
regulation of mRNA metabolic process
regulation of chromosome organization
regulation of intracellular transport
RNA splicing
mRNA processing
transcription by RNA polymerase II
regulation of cellular protein localization
transcription, DNA-templated
nucleic acid-templated transcription
RNA biosynthetic process
mRNA metabolic process
regulation of protein transport
regulation of peptide transport
regulation of establishment of protein localization
RNA processing