Chloramphenicol 20μM R00 exp13

Chloramphenicol 20μM R00 exp13

Mechanism of Action

Binds 23S rRNA of 50S ribosomal subunit, inhibits protein synthesis

Class / Subclass 1: Infectious Disease / Antibiotic
Class / Subclass 2: Organelle Function / Mitochondrial Inhibitor

Technical Notes

Protein References

PubChem Name: Chloramphenicol
Synonyms: N/A
CAS #: 56-75-7
PubChem CID: 5959
IUPAC: 2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide
INCHI Name: InChI=1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)/t8-,9-/m1/s1
INCHI Key: WIIZWVCIJKGZOK-RKDXNWHRSA-N
Molecular Weight: 323.13
Canonical SMILES: C1=CC(=CC=C1C(C(CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]
Isomeric SMILES: C1=CC(=CC=C1[C@H]([C@@H](CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]
Molecular Formula: C11H12Cl2N2O5

Protein Supplier

Supplier Name: Sigma-Aldrich
Catalog #: C0378
Lot #: 096K1244

Protein Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C11H12Cl2N2O5 323.0196; found 323.01899

Dose Response Curve

Platform ID: Chloramphenicol
Min: -5.6180; Max: 8.0817

IC	Concentration (µM)
IC10	N/A
IC20	N/A
IC30	N/A
IC40	N/A
IC50	N/A
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 00
Dose: 20µM
Days of incubation: 8
Doublings: 4.8
Numbers of reads: 10775991

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
3/18	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
GOT1	-4.37	<0.01
NUDT5	-2.91	0.01
HARS	-2.82	0.37
AURKB	-2.76	0.37
PDXK	-2.72	0.03
PRMT5	-2.68	0.44
ORC3	-2.49	0.37
KDM2B	-2.32	0.37
UNK	-2.25	0.37
HPRT1	-2.23	0.37
FAM132B	-2.23	0.37
RACGAP1	-2.16	0.37
UROD	-2.15	0.44
C17orf82	-2.11	0.37
SLC4A7	-2.09	0.37
XPO5	-2.08	0.51
CPXM1	-2.05	0.37
ECHS1	-2.05	0.37
ZFAND4	-2.02	0.37
PHF15	-2.00	0.37
DGAT1	-2.00	0.42
HADH	-2.00	0.37
KIF23	-1.99	0.37
CARKD	-1.99	0.37
YTHDF1	-1.98	0.37
LAMTOR4	-1.98	0.42
MAP6D1	-1.97	0.42
ADRA1B	-1.97	0.42
PCK1	-1.96	0.43
PIGY	-1.95	0.42
ACAD9	2.29	0.06
SMG7	2.31	0.39
NDUFS1	2.32	0.10
PRKCA	2.32	0.06
CHMP2A	2.38	0.37
SLC25A52	2.40	0.04
ATP5E	2.40	0.12
NFE2L1	2.42	0.07
CD79B	2.43	0.11
RPL23	2.46	0.26
CWC22	2.52	0.03
MTIF2	2.53	0.05
ATP5O	2.53	0.03
TMEM165	2.57	0.04
ATP5A1	2.61	0.03
CKAP5	2.62	0.29
VCP	2.63	0.03
ATP5L	2.65	0.05
IPPK	2.67	0.02
NDUFC2	2.69	0.02
NR2C2AP	2.71	0.03
KIF11	2.73	0.37
SNRNP70	2.74	0.03
MYCBP2	2.75	0.03
IPMK	2.80	0.02
STARD7	2.81	0.02
MDH1	2.97	0.01
G3BP1	3.18	<0.01
ITPK1	3.31	<0.01
SLC25A51	3.92	<0.01

Screen	Correlation	Plot
QNZ 0.01μM R08 exp439	0.107	View
Mubritinib 0.2μM R01 exp48	0.095	View
Mubritinib 0.2μM R02 exp65	0.094	View
Phenformin 20μM R05 exp212	0.09	View
QNZ 0.01μM R05 exp241	0.084	View
Rotenone 0.07μM R08 exp431	0.076	View
Deguelin 0.15μM R05 exp209	0.073	View
Oligomycin-A 2μM R00 exp25	0.065	View
NMS-873 0.07μM R07 exp380	0.052	View
Rapamycin 1μM R00 exp29	0.052	View

GO Term	Fold Change	Genes
mitotic spindle midzone assembly	177.65	AURKB,RACGAP1,KIF23
spindle elongation	148.04	AURKB,RACGAP1,KIF23
spindle midzone assembly	136.66	AURKB,RACGAP1,KIF23

GO Term	Fold Change	Genes
proton-transporting ATP synthase activity, rotational mechanism	157.91	ATP5MG,ATP5F1A,ATP5PO,ATP5F1E
mitochondrial proton-transporting ATP synthase complex	147.39	ATP5MG,ATP5F1A,ATP5PO,ATP5F1E
proton motive force-driven mitochondrial ATP synthesis	62.57	NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
proton motive force-driven ATP synthesis	55.27	NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
ATP biosynthetic process	47.37	NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
purine ribonucleoside triphosphate biosynthetic process	40.94	NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
purine nucleoside triphosphate biosynthetic process	40.44	NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
oxidative phosphorylation	39.08	SLC25A51,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
ribonucleoside triphosphate biosynthetic process	38.56	NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
ATP metabolic process	38.31	NDUFC2,ATP5MG,VCP,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
aerobic respiration	31.36	SLC25A51,MDH1,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
purine ribonucleoside triphosphate metabolic process	30.95	NDUFC2,ATP5MG,VCP,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
purine nucleoside triphosphate metabolic process	29.76	NDUFC2,ATP5MG,VCP,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
ribonucleoside triphosphate metabolic process	29.53	NDUFC2,ATP5MG,VCP,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
nucleoside triphosphate metabolic process	26.50	NDUFC2,ATP5MG,VCP,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
cellular respiration	26.16	SLC25A51,MDH1,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
energy derivation by oxidation of organic compounds	18.90	SLC25A51,MDH1,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,NDUFS1
mitochondrial membrane	9.39	SLC25A51,STARD7,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,SLC25A52,PRKCA,NDUFS1,ALAS1,ACAD9
mitochondrial envelope	8.82	SLC25A51,STARD7,NDUFC2,ATP5MG,ATP5F1A,ATP5PO,ATP5F1E,SLC25A52,PRKCA,NDUFS1,ALAS1,ACAD9

Table of Contents

Chloramphenicol 20μM R00 exp13

Mechanism of Action

Technical Notes

Protein References

Protein Supplier

Protein Characterization

Dose Response Curve

Screen Summary

Screen Results