Cytochalasin-B 5μM R07 exp349

Cytochalasin-B 5μM R07 exp349

Mechanism of Action

Binds fast-growing (barbed) end of F-actin filament, inhibits actin polymerization

Class / Subclass 1: Cell Cycle / Cytokinesis Inhibitor
Class / Subclass 2: Organelle Function / Cytoskeletal Inhibitor
Class / Subclass 3: Environmental Stresses / Toxin

Technical Notes

Protein References

PubChem Name: Cytochalasin B
Synonyms: Phomin
CAS #: 14930-96-2
PubChem CID: 5311281
IUPAC: (1S,4E,6R,10R,12E,14S,15S,17S,18S,19S)-19-benzyl-6,15-dihydroxy-10,17-dimethyl-16-methylidene-2-oxa-20-azatricyclo[12.7.0.01,18]henicosa-4,12-diene-3,21-dione
INCHI Name: InChI=1S/C29H37NO5/c1-18-9-7-13-22(31)15-16-25(32)35-29-23(14-8-10-18)27(33)20(3)19(2)26(29)24(30-28(29)34)17-21-11-5-4-6-12-21/h4-6,8,11-12,14-16,18-19,22-24,26-27,31,33H,3,7,9-10,13,17H2,1-2H3,(H,30,34)/b14-8+,16-15+/t18-,19-,22-,23+,24+,26+,27-,29-/m1/s1
INCHI Key: GBOGMAARMMDZGR-TYHYBEHESA-N
Molecular Weight: 479.6
Canonical SMILES: CC1CCCC(C=CC(=O)OC23C(C=CC1)C(C(=C)C(C2C(NC3=O)CC4=CC=CC=C4)C)O)O
Isomeric SMILES: C[C@@H]1CCC[C@H](/C=C/C(=O)O[C@]23[C@@H](/C=C/C1)[C@@H](C(=C)[C@H]([C@H]2[C@@H](NC3=O)CC4=CC=CC=C4)C)O)O
Molecular Formula: C29H37NO5

Protein Supplier

Supplier Name: Sigma-Aldrich
Catalog #: C6762
Lot #: 086M4129V

Protein Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C29H37NO5 480.27445; found 480.27738

Dose Response Curve

Platform ID: Cytochalasin_B
Min: -24.8534; Max: 80.8909

IC	Concentration (µM)
IC10	N/A
IC20	0.6851
IC30	1.3466
IC40	2.6729
IC50	5.6719
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 07
Dose: 5µM
Days of incubation: 8
Doublings: -0.1
Numbers of reads: 21844473

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
0/71	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
USP5	-2.75	0.08
ZNF300	-2.66	0.08
VDAC1	-2.58	0.08
NHP2L1	-2.58	0.40
NFS1	-2.51	0.42
ATP6V0B	-2.43	0.33
ABCD1	-2.39	0.18
ARAP2	-2.34	0.18
FAM89B	-2.29	0.18
ZBTB1	-2.28	0.18
RCOR1	-2.28	0.18
VPS25	-2.24	0.45
PSMA2	-2.23	0.40
DDX3X	-2.21	0.24
KPNB1	-2.18	0.60
CDC16	-2.17	0.31
PPP2R2A	-2.15	0.24
PRPF4	-2.15	0.61
RSPH4A	-2.15	0.40
NEK11	-2.15	0.32
GATAD2A	-2.15	0.24
ZNF816-ZNF321P	-2.14	0.24
CCM2	-2.14	0.24
HSPA9	-2.13	0.69
SATB2	-2.13	0.24
JAM2	-2.10	0.34
POLR3G	-2.08	0.36
NDUFC2	-2.08	0.30
R3HDM1	-2.07	0.30
CDK5RAP2	-2.06	0.36
RTTN	3.21	0.00
RBM26	3.32	0.00
BAK1	3.35	0.00
ELAVL1	3.37	0.00
ATMIN	3.40	0.00
TUBE1	3.41	0.00
EIF4A1	3.52	0.00
CEP78	3.75	0.00
CNTRL	3.92	0.00
FOPNL	3.93	0.00
CEP44	3.93	0.00
BUB3	3.95	0.00
C16orf59	4.16	0.00
SCLT1	4.37	0.00
NIN	4.51	0.00
ANKRD26	4.56	0.00
CEP120	4.57	0.00
CEP76	4.65	0.00
TUBD1	4.88	0.00
CRADD	4.98	0.00
C14orf80	5.15	0.00
ODF2	5.23	0.00
CEP128	5.32	0.00
PIDD	5.81	0.00
CASP2	6.29	0.00
OFD1	6.31	0.00
PIBF1	6.48	0.00
CCDC41	6.58	0.00
C2CD3	6.67	0.00
KIAA0753	6.75	0.00

Screen	Correlation	Plot
Latrunculin-B 10μM R07 exp374	0.273	View
Tozasertib 0.1μM R07 exp414	0.223	View
RO-3307 4.7μM R02 exp80	0.118	View
Olaparib 4μM R08 exp512	0.066	View
GSK626616 14μM R08 exp485	0.061	View
Hippuristanol 0.12μM R08 exp488	0.055	View
Staurosporine 0.02μM R08 exp524	0.052	View
Centrinone 0.5μM R07 exp343	0.072	View
2-Deoxy-D-glucose 800μM R05 exp214	0.055	View

GO Term	Fold Change	Genes
ciliary transition fiber	236.84	CEP83,NIN,SCLT1
centriole-centriole cohesion	184.21	ODF2,NIN,CEP44,RTTN
centriole replication	115.13	KIAA0753,C2CD3,OFD1,CEP44,RTTN
protein localization to centrosome	100.48	KIAA0753,C2CD3,CEP83,PIBF1
centriole assembly	89.13	KIAA0753,C2CD3,OFD1,CEP44,RTTN
centrosome duplication	78.95	KIAA0753,C2CD3,OFD1,CEP44,RTTN
centriole	56.39	KIAA0753,C2CD3,CEP83,OFD1,CEP128,ODF2,TUBD1,CEP76,CEP120,NIN,SCLT1,CEP44,CEP20,CEP78,RTTN
centrosome cycle	55.26	KIAA0753,C2CD3,OFD1,ODF2,CEP120,NIN,CEP44,TUBE1,RTTN
microtubule organizing center organization	46.48	KIAA0753,C2CD3,OFD1,ODF2,CEP120,NIN,CEP44,TUBE1,RTTN
centriolar satellite	29.09	KIAA0753,C2CD3,PIBF1,OFD1,CEP20,CNTRL
cilium organization	18.82	KIAA0753,C2CD3,CEP83,PIBF1,OFD1,ODF2,SCLT1,CEP20,CEP78,ATMIN,RTTN
centrosome	16.20	KIAA0753,C2CD3,CEP83,PIBF1,OFD1,CEP128,ODF2,CEP76,CEP120,ANKRD26,NIN,SCLT1,CEP44,CEP20,CNTRL,CEP78,TUBE1,RTTN
cilium assembly	14.99	KIAA0753,C2CD3,CEP83,PIBF1,OFD1,SCLT1,CEP20,ATMIN
microtubule organizing center	13.04	KIAA0753,C2CD3,CEP83,PIBF1,OFD1,CEP128,ODF2,TUBD1,CEP76,CEP120,ANKRD26,NIN,SCLT1,CEP44,CEP20,CNTRL,CEP78,TUBE1,RTTN
microtubule cytoskeleton organization	12.33	KIAA0753,C2CD3,PIBF1,OFD1,ODF2,TUBD1,CEP120,NIN,CEP44,CEP20,TUBE1,RTTN
plasma membrane bounded cell projection assembly	11.19	KIAA0753,C2CD3,CEP83,PIBF1,OFD1,SCLT1,CEP20,ATMIN
cilium	9.77	C2CD3,CEP83,OFD1,CEP128,ODF2,TUBD1,NIN,SCLT1,CEP20,CNTRL,CEP78,RTTN
cell cycle process	8.87	KIAA0753,C2CD3,PIBF1,OFD1,CASP2,PIDD1,ODF2,CRADD,CEP120,NIN,BUB3,CEP44,TUBE1,RTTN
microtubule-based process	8.51	KIAA0753,C2CD3,PIBF1,OFD1,ODF2,TUBD1,CEP120,NIN,CEP44,CEP20,TUBE1,RTTN

Table of Contents

Cytochalasin-B 5μM R07 exp349

Mechanism of Action

Technical Notes

Protein References

Protein Supplier

Protein Characterization

Dose Response Curve

Screen Summary

Screen Results