Staurosporine 0.02μM R08 exp524

Inhibits many protein kinases including PKC, PKA, tyrosine kinases, CDKs, others

Class / Subclass 1: Signal Transduction / Kinase Inhibitor

Compound References

PubChem Name: Staurosporine
Synonyms: Antibiotic AM-2282; STS; AM-2282
CAS #: 62996-74-1
PubChem CID: 44259
IUPAC: (2S,3R,4R,6R)-3-methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.12,6.07,28.08,13.015,19.020,27.021,26]nonacosa-8,10,12,14,19,21,23,25,27-nonaen-16-one
INCHI Name: InChI=1S/C28H26N4O3/c1-28-26(34-3)17(29-2)12-20(35-28)31-18-10-6-4-8-14(18)22-23-16(13-30-27(23)33)21-15-9-5-7-11-19(15)32(28)25(21)24(22)31/h4-11,17,20,26,29H,12-13H2,1-3H3,(H,30,33)/t17-,20-,26-,28+/m1/s1
INCHI Key: HKSZLNNOFSGOKW-FYTWVXJKSA-N
Molecular Weight: 466.5
Canonical SMILES: CC12C(C(CC(O1)N3C4=CC=CC=C4C5=C6C(=C7C8=CC=CC=C8N2C7=C53)CNC6=O)NC)OC
Isomeric SMILES: C[C@@]12[C@@H]([C@@H](C[C@@H](O1)N3C4=CC=CC=C4C5=C6C(=C7C8=CC=CC=C8N2C7=C53)CNC6=O)NC)OC
Molecular Formula: C28H26N4O3

Compound Supplier

Supplier Name: Med Chem Express
Catalog #: HY-15141
Lot #: 32940

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C28H26N4O3 467.20777; found 467.20856

Dose Response Curve

Platform ID: Staurosporin
Min: -7.4960; Max: 99.8510

IC	Concentration (µM)
IC10	0.0059
IC20	0.0122
IC30	0.0197
IC40	0.0292
IC50	0.0419
IC60	0.0602
IC70	0.0892
IC80	0.1443
IC90	0.2973

Screen Summary

Round: 08
Dose: 20nM
Days of incubation: 8
Doublings: 0.5
Numbers of reads: 17250856

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
2/66	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
OTUB1	-3.45	<0.01
EP300	-2.85	0.12
MBTD1	-2.81	0.03
KCTD10	-2.56	0.09
ATP6V0A2	-2.54	0.11
CWC27	-2.51	0.09
CMYA5	-2.48	0.23
IPO11	-2.46	0.09
PSMC5	-2.44	0.38
CDK19	-2.42	0.11
CENPE	-2.38	0.18
CHMP7	-2.37	0.11
INCENP	-2.33	0.37
SOX4	-2.30	0.30
RAD21	-2.22	0.36
DRD3	-2.15	0.30
MORF4L1	-2.14	0.27
FAM217A	-2.11	0.33
GPX4	-2.11	0.29
HNRNPD	-2.09	0.29
CSE1L	-2.09	0.49
UBE2N	-2.09	0.29
MED12	-2.08	0.49
DUSP23	-2.06	0.30
MLLT10	-2.05	0.30
EPAS1	-2.05	0.37
ZBTB7A	-2.04	0.30
EIF3L	-2.04	0.40
PSMB1	-2.03	0.41
GPR101	-2.02	0.30
NSD1	2.83	<0.01
FAM204A	2.86	<0.01
HDAC5	2.87	<0.01
FBXO11	2.87	<0.01
ROMO1	2.89	<0.01
TSR2	2.92	0.09
MAP2K5	3.04	<0.01
TAZ	3.04	0.02
SRM	3.17	0.00
ARID4B	3.21	0.00
NCOR1	3.24	0.00
LPAR5	3.25	0.00
CASP9	3.26	0.00
KMT2D	3.36	0.00
RBM12	3.37	0.00
YLPM1	3.49	<0.01
MAPK7	3.77	0.00
VDAC2	3.94	0.00
FAF2	3.99	0.00
BUB3	4.04	0.00
PRKACA	4.08	0.00
MIDN	4.12	0.00
PLAA	4.13	0.00
ATMIN	4.32	0.00
CXorf56	4.32	0.00
GNAS	4.75	0.00
UBQLN1	4.94	0.00
APAF1	5.05	0.00
MARCH5	5.12	0.00
BAK1	7.39	0.00

Screen	Correlation	Plot
Tanespimycin 14μM R08 exp527	0.077	View
THZ1 0.06μM R08 exp531	0.077	View
Thimerosal 0.85μM R08 exp529	0.065	View
UM0129480 7μM R02 exp85	0.061	View
Benzoate 20000μM R08 exp456	0.132	View
Hippuristanol 0.12μM R08 exp489 no dilution day6	0.12	View
Hippuristanol 0.12μM R08 exp488	0.118	View
Mitomycin-C 0.06μM R08 exp503	0.116	View
Bleomycin 5μM R08 exp459	0.115	View
MS023 7μM R03 exp135	0.105	View
Milciclib 2μM R08 exp502	0.101	View
GSK626616 14μM R08 exp485	0.101	View
Aphidicolin 0.4μM R08 exp440	0.1	View
Actinomycin-D 0.001μM R00 exp3	0.096	View
MS023 2μM R03 exp134	0.094	View
Doxorubicin 0.02μM R08 exp478	0.093	View
PU-H71 1μM R08 exp515	0.091	View
RO-3307 4.7μM R02 exp80	0.09	View
CR131-b 0.005μM R08 exp474	0.089	View
Etoposide 0.3μM R05 exp199	0.088	View
JQ1 0.8μM R08 exp435	0.084	View
Dorsomorphin 5μM R07 exp357	0.079	View
Pimelic-diphenylamide-106 5μM R00 exp28	0.079	View
Latrunculin-B 10μM R07 exp374	0.078	View
AICAR 240μM R05 exp211	0.075	View
SNS-032 15μM R08 exp443	0.075	View
Dinaciclib 0.007μM R07 exp355	0.074	View
Ethanol 0.01 R05 exp234	0.073	View
Olaparib 4μM R08 exp512	0.072	View
ML-792 0.2μM R08 exp505	0.071	View
5-Fluorouracil 20μM R00 exp2	0.071	View
Rotenone 20μM R00 exp34	0.07	View
Etoposide 1μM R00 exp19	0.07	View
CB-5083 0.4μM R08 exp468	0.066	View
Erlotinib 10μM R05 exp216	0.061	View
CI-1040 9.5μM R08 exp472	0.061	View
6-Thio-2-deoxyguanosine 2μM R06 exp259	0.06	View
Atovaquone 15μM R08 exp451	0.052	View
Cytochalasin-B 5μM R07 exp349	0.052	View
Artemisinin 50μM R08 exp450	0.051	View
AICAR 240μM R03 exp116	0.051	View

Staurosporine 0.02μM R08 exp524

Mechanism of Action

Technical Notes

Compound References

Compound Supplier

Compound Characterization

Dose Response Curve

Screen Summary

Screen Results