Brefeldin A 0.02μM R00 exp8
Mechanism of Action
Inhibits Golgi to ER transport, forms uncompetitive complex with GBF1 and GDP-bound Arf1
- Class / Subclass 1: Organelle Function / Vesicule Trafficking Inhibitor
Technical Notes
Compound References
- PubChem Name: Brefeldin A
- Synonyms: BFA; Cyanein; Decumbin
- CAS #: 20350-15-6
- PubChem CID: 5287620
- IUPAC: (1R,2R,3E,7S,11E,13S,15S)-2,15-dihydroxy-7-methyl-6-oxabicyclo[11.3.0]hexadeca-3,11-dien-5-one
- INCHI Name: InChI=1S/C16H24O4/c1-11-5-3-2-4-6-12-9-13(17)10-14(12)15(18)7-8-16(19)20-11/h4,6-8,11-15,17-18H,2-3,5,9-10H2,1H3/b6-4+,8-7+/t11-,12+,13-,14+,15+/m0/s1
- INCHI Key: KQNZDYYTLMIZCT-KQPMLPITSA-N
- Molecular Weight: 280.36
- Canonical SMILES: CC1CCCC=CC2CC(CC2C(C=CC(=O)O1)O)O
- Isomeric SMILES: C[C@H]1CCC/C=C/[C@@H]2C[C@@H](C[C@H]2[C@@H](/C=C/C(=O)O1)O)O
- Molecular Formula: C16H24O4
Compound Supplier
- Supplier Name: LC Laboratories
- Catalog #: B-8500
- Lot #: BBF-401
Compound Characterization
- HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C16H24O4 281.17474; found 281.17412
Dose Response Curve
- Platform ID: Brefeldin_A
- Min: -14.8343; Max: 90.1805
| IC | Concentration (µM) |
|---|---|
| IC10 | N/A |
| IC20 | 0.1694 |
| IC30 | 0.1783 |
| IC40 | 0.1859 |
| IC50 | 0.1931 |
| IC60 | N/A |
| IC70 | N/A |
| IC80 | N/A |
| IC90 | N/A |
Screen Summary
- Round: 00
- Dose: 20nM
- Days of incubation: 8
- Doublings: 0.6
- Numbers of reads: 11323380
Screen Results
| Sensitive/Resistant hits (FDR<0.05) | CRANKS | Score Plot | Top 30 Genes | Screen Similarity | Top 30 Sensitive GO terms | Top 30 Resistant GO terms |
|---|---|---|---|---|---|---|
| 7/0 | Scores |
Brefeldin A 0.02μM R00 exp8
Brefeldin A 0.02μM R00 exp8 vs DABN 20μM R00 exp17
Brefeldin A 0.02μM R00 exp8 vs Oligomycin-A 20μM R00 exp26
Bortezomib 0.05μM R00 exp7 vs Brefeldin A 0.02μM R00 exp8
