Ribavirin 10 to 15μM on day4 R04 exp160

Ribavirin 10 to 15μM on day4 R04 exp160

Mechanism of Action

Prodrug metabolized to purine RNA nucleotide mimetic, also inhibits EIF4E

Class / Subclass 1: Infectious Disease / Antiviral
Class / Subclass 2: Metabolism / Antimetabolite
Class / Subclass 3: DNA Damage, Repair and Replication / Reverse Transcriptase Inhibitor

Technical Notes

Protein References

PubChem Name: Ribavirin
Synonyms: ICN-1229
CAS #: 36791-04-5
PubChem CID: 37542
IUPAC: 1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,2,4-triazole-3-carboxamide
INCHI Name: InChI=1S/C8H12N4O5/c9-6(16)7-10-2-12(11-7)8-5(15)4(14)3(1-13)17-8/h2-5,8,13-15H,1H2,(H2,9,16)/t3-,4-,5-,8-/m1/s1
INCHI Key: IWUCXVSUMQZMFG-AFCXAGJDSA-N
Molecular Weight: 244.2
Canonical SMILES: C1=NC(=NN1C2C(C(C(O2)CO)O)O)C(=O)N
Isomeric SMILES: C1=NC(=NN1[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O)C(=O)N
Molecular Formula: C8H12N4O5

Protein Supplier

Supplier Name: Kemprotec Ltd
Catalog #: K-1104
Lot #: 170221

Protein Characterization

Characterization data not available.

Dose Response Curve

Platform ID: Ribavirin
Min: -21.0950; Max: 37.2497

IC	Concentration (µM)
IC10	N/A
IC20	30.6995
IC30	36.4591
IC40	N/A
IC50	N/A
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 04
Dose: 10-15µM
Days of incubation: 8
Doublings: 6.6
Numbers of reads: 14345761

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
18/8	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
AMPD2	-5.11	<0.01
SEPSECS	-3.15	<0.01
PRPS1	-3.11	<0.01
GPR107	-3.05	<0.01
SUMO2	-2.91	0.11
ARL1	-2.80	0.01
RALA	-2.71	0.02
MBTPS1	-2.69	0.05
KDSR	-2.65	0.02
PCGF6	-2.64	0.02
HPRT1	-2.59	0.04
FBXW7	-2.58	0.03
TCF4	-2.56	0.03
KMT2D	-2.55	0.03
SIVA1	-2.47	0.03
GCLC	-2.46	0.03
LAMTOR4	-2.43	0.04
ZFYVE20	-2.40	0.13
ATP6V1G1	-2.40	0.08
EIF4A1	-2.38	0.05
EXOC2	-2.37	0.05
PRRC2C	-2.35	0.15
XPOT	-2.28	0.11
TRAPPC3	-2.27	0.08
ATP2A2	-2.25	0.26
RAET1G	-2.20	0.21
LACC1	-2.20	0.11
IVNS1ABP	-2.17	0.11
SEPHS1	-2.16	0.11
TEN1	-2.16	0.11
C10orf2	2.15	0.16
COX18	2.15	0.16
FAM129C	2.15	0.20
ZNF317	2.17	0.20
NFU1	2.18	0.31
UQCC1	2.18	0.20
TRUB1	2.20	0.14
APOBEC3F	2.22	0.19
CD1C	2.25	0.17
ATP6AP2	2.26	0.21
COQ4	2.27	0.11
DDX54	2.29	0.20
MRPL48	2.32	0.23
FOXRED1	2.34	0.08
SHD	2.38	0.07
COX15	2.42	0.06
PIK3C2A	2.43	0.06
NDUFA5	2.49	0.05
NDUFAF3	2.52	0.13
COX6C	2.53	0.19
ADSS	2.54	0.23
RPL30	2.56	0.45
NUBPL	2.60	0.04
YBEY	2.60	0.06
CDK13	2.74	0.04
PET117	2.78	0.01
NDUFA2	3.02	0.01
MTO1	3.43	<0.01
NUDT2	5.35	<0.01
ADK	8.18	<0.01

Screen	Correlation	Plot
Ribavirin 10μM R01 exp52	0.06	View
HMS-I2 10μM R06 exp288	0.066	View
Genistein 15μM R07 exp360	0.055	View
Oligomycin-A 2μM R00 exp25	0.055	View
BH1 1μM R04 exp169	0.051	View

GO Term	Fold Change	Genes
hypoxanthine biosynthetic process	571.52	PRPS1,HPRT1

GO Term	Fold Change	Genes
cytochrome complex assembly	65.00	PET117,COX15,UQCC1,COX18
mitochondrial respiratory chain complex I assembly	63.75	NDUFA2,NUBPL,NDUFAF3,NDUFA5,FOXRED1,NDUFA9
NADH dehydrogenase complex assembly	63.75	NDUFA2,NUBPL,NDUFAF3,NDUFA5,FOXRED1,NDUFA9
mitochondrial respiratory chain complex assembly	57.15	NDUFA2,PET117,NUBPL,NDUFAF3,NDUFA5,FOXRED1,UQCC1,NDUFA9,COX18
mitochondrial respirasome	44.20	NDUFA2,COX6C,NDUFA5,COX15,FOXRED1,NDUFA9
respirasome	40.42	NDUFA2,COX6C,NDUFA5,COX15,FOXRED1,NDUFA9
aerobic electron transport chain	37.84	NDUFA2,COX6C,NDUFA5,COX15,NDUFA9
respiratory chain complex	37.33	NDUFA2,COX6C,NDUFA5,FOXRED1,NDUFA9
mitochondrial ATP synthesis coupled electron transport	36.35	NDUFA2,COX6C,NDUFA5,COX15,NDUFA9
purine nucleoside triphosphate biosynthetic process	34.10	ADK,NUDT2,NDUFA2,NDUFA5,NDUFA9
nucleoside triphosphate biosynthetic process	29.70	ADK,NUDT2,NDUFA2,NDUFA5,NDUFA9
aerobic respiration	23.51	NDUFA2,COX6C,NDUFA5,COX15,NDUFA9,MDH2
cellular respiration	19.61	NDUFA2,COX6C,NDUFA5,COX15,NDUFA9,MDH2
purine ribonucleotide biosynthetic process	19.05	ADK,NUDT2,NDUFA2,ADSS2,NDUFA5,NDUFA9
mitochondrial inner membrane	13.27	NDUFA2,COX6C,NDUFAF3,NDUFA5,COX15,FOXRED1,MRPL48,COQ4,UQCC1,NDUFA9,COX18
organelle inner membrane	11.92	NDUFA2,COX6C,NDUFAF3,NDUFA5,COX15,FOXRED1,MRPL48,COQ4,UQCC1,NDUFA9,COX18
mitochondrion organization	11.84	NDUFA2,PET117,NUBPL,NDUFAF3,NDUFA5,FOXRED1,UQCC1,NDUFA9,COX18
mitochondrial membrane	8.62	NDUFA2,COX6C,NDUFAF3,NDUFA5,COX15,FOXRED1,MRPL48,COQ4,UQCC1,NDUFA9,COX18
mitochondrial envelope	8.09	NDUFA2,COX6C,NDUFAF3,NDUFA5,COX15,FOXRED1,MRPL48,COQ4,UQCC1,NDUFA9,COX18

Table of Contents

Ribavirin 10 to 15μM on day4 R04 exp160

Mechanism of Action

Technical Notes

Protein References

Protein Supplier

Protein Characterization

Dose Response Curve

Screen Summary

Screen Results