CoCl2 18μM R07 exp346

CoCl2 18μM R07 exp346

Mechanism of Action

Hypoxia mimetic, inhibits VHL-HIF-1α interaction, induces HIF-1α accumulation

Class / Subclass 1: Environmental Stresses / Heavy Metal
Class / Subclass 2: Metabolism / Metabolic Enzyme Inhibitor

Technical Notes

Protein References

PubChem Name: Cobalt dichloride hexahydrate
Synonyms: N/A
CAS #: 7791-13-1
PubChem CID: 24643
IUPAC: cobalt(2+);dichloride;hexahydrate
INCHI Name: InChI=1S/2ClH.Co.6H2O/h2*1H;;6*1H2/q;;+2;;;;;;/p-2
INCHI Key: GFHNAMRJFCEERV-UHFFFAOYSA-L
Molecular Weight: 237.93
Canonical SMILES: O.O.O.O.O.O.[Cl-].[Cl-].[Co+2]
Isomeric SMILES: N/A
Molecular Formula: Cl2CoH12O6

Protein Supplier

Supplier Name: Sigma-Aldrich
Catalog #: 255599
Lot #: 04324JB

Protein Characterization

XRPD: Expected CoCl2 6H2O; found CoCl2 2H2O

Dose Response Curve

Platform ID: Cobalt_chloride
Min: -6.9301; Max: 88.2117

IC	Concentration (µM)
IC10	N/A
IC20	11.8286
IC30	16.0679
IC40	21.1851
IC50	27.8871
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 07
Dose: 18µM
Days of incubation: 8
Doublings: 1.7
Numbers of reads: 20414333

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
34/37	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
SLC39A9	-4.33	<0.01
SLC30A1	-3.31	<0.01
HIF1AN	-3.05	<0.01
AP1G1	-2.95	<0.01
PACS1	-2.89	0.03
HEXIM1	-2.86	<0.01
ABCB7	-2.85	<0.01
SLC39A10	-2.82	<0.01
SLC7A11	-2.79	<0.01
MBNL1	-2.77	<0.01
ATP6V0A2	-2.66	0.02
SH3GL1	-2.66	0.03
TFRC	-2.66	0.23
SLC35E1	-2.64	0.01
IREB2	-2.62	0.02
TCF4	-2.60	0.02
C7orf55-LUC7L2	-2.56	0.02
SLC10A7	-2.55	0.02
SPEN	-2.54	0.02
NUBP1	-2.52	0.03
RBM26	-2.52	0.03
LARP7	-2.52	0.02
CCDC22	-2.50	0.02
PSMB8	-2.47	0.02
UBE2A	-2.46	0.02
POLE3	-2.42	0.03
RAB14	-2.41	0.03
CYB561A3	-2.40	0.05
NID1	-2.39	0.03
UBE2E1	-2.37	0.03
TAMM41	2.42	0.02
PITRM1	2.46	0.02
CSNK2A3	2.46	0.04
MSL1	2.54	0.03
SLC39A14	2.57	0.01
MRPL23	2.60	0.01
MOGS	2.69	0.01
MBTPS1	2.69	0.03
LAP3	2.74	<0.01
SEC16A	2.74	0.05
RABL6	2.76	<0.01
STRAP	2.77	<0.01
USO1	2.79	<0.01
PTPMT1	2.82	0.02
UGP2	2.86	<0.01
BACH2	2.90	<0.01
IBA57	2.91	<0.01
NAIF1	2.92	<0.01
RBM10	3.04	<0.01
DNAJC13	3.13	0.00
SCAP	3.17	0.00
ATXN7L3	3.28	0.00
PYROXD1	3.30	<0.01
PGM3	3.41	0.00
SLC39A6	3.84	0.00
MAT2B	4.11	0.00
TMEM165	4.36	0.00
ATP2C1	4.50	0.00
SEC31A	4.75	0.00
SLC39A8	8.50	0.00

Screen	Correlation	Plot
Hydroxyurea 100μM R07 exp329	0.079	View
TIC10 10μM R08 exp532	0.051	View
ABT-702 5μM plus CoCl2 18μM R07 exp320	0.181	View
ABT-702 5μM plus Deferoxamine 11μM R07 exp321	0.068	View
Bafilomycin-A1 0.009μM R08 exp454	0.063	View
BML-284 0.09μM R08 exp460	0.054	View
Aphidicolin 0.4μM R08 exp440	0.052	View

GO Term	Fold Change	Genes
zinc ion transmembrane transporter activity	77.23	SLC39A9,SLC30A1,SLC39A10
intracellular zinc ion homeostasis	74.01	SLC39A9,SLC30A1,SLC39A10
zinc ion transmembrane transport	68.32	SLC39A9,SLC30A1,SLC39A10
zinc ion transport	65.79	SLC39A9,SLC30A1,SLC39A10
intracellular iron ion homeostasis	52.24	ABCB7,ATP6V0A2,TFRC,IREB2,NUBP1,CYB561A3
transition metal ion transmembrane transporter activity	43.32	SLC39A9,SLC30A1,SLC39A10
transition metal ion transport	31.50	SLC39A9,SLC30A1,ABCB7,SLC39A10,TFRC
intracellular monoatomic cation homeostasis	16.12	SLC39A9,SLC30A1,ABCB7,SLC39A10,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3
inorganic ion homeostasis	15.81	SLC39A9,SLC30A1,ABCB7,SLC39A10,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3
intracellular monoatomic ion homeostasis	15.77	SLC39A9,SLC30A1,ABCB7,SLC39A10,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3
monoatomic cation homeostasis	13.65	SLC39A9,SLC30A1,ABCB7,SLC39A10,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3
monoatomic ion homeostasis	13.40	SLC39A9,SLC30A1,ABCB7,SLC39A10,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3
intracellular chemical homeostasis	13.38	SLC39A9,SLC30A1,ABCB7,SLC39A10,SLC7A11,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3
cellular homeostasis	11.14	SLC39A9,SLC30A1,ABCB7,SLC39A10,SLC7A11,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3
active transmembrane transporter activity	10.77	SLC30A1,ABCB7,SLC39A10,SLC7A11,ATP6V0A2,SLC35E1,SLC10A7,CYB561A3
chemical homeostasis	8.50	SLC39A9,SLC30A1,ABCB7,SLC39A10,SLC7A11,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3

GO Term	Fold Change	Genes
cadmium ion transmembrane transport	221.05	SLC39A8,SLC39A14
cadmium ion transport	221.05	SLC39A8,SLC39A14
manganese ion transport	221.05	SLC39A8,ATP2C1,TMEM165,SLC39A14
manganese ion transmembrane transporter activity	207.24	ATP2C1,TMEM165,SLC39A14
iron import into cell	184.21	SLC39A8,SLC39A14
manganese ion transmembrane transport	184.21	SLC39A8,ATP2C1,TMEM165
monoatomic cation:bicarbonate symporter activity	138.16	SLC39A8,SLC39A6,SLC39A14
zinc ion transmembrane transporter activity	72.08	SLC39A8,SLC39A6,SLC39A14
intracellular zinc ion homeostasis	69.08	SLC39A8,SLC39A6,SLC39A14
transition metal ion transmembrane transporter activity	67.39	SLC39A8,ATP2C1,TMEM165,SLC39A6,SLC39A14
zinc ion transmembrane transport	63.77	SLC39A8,SLC39A6,SLC39A14
zinc ion transport	61.40	SLC39A8,SLC39A6,SLC39A14
bicarbonate transmembrane transporter activity	59.21	SLC39A8,SLC39A6,SLC39A14
bicarbonate transport	50.24	SLC39A8,SLC39A6,SLC39A14
COPII-coated vesicle budding	43.63	SEC31A,SCAP,SEC16A
ER to Golgi transport vesicle membrane	37.47	SEC31A,SCAP,USO1,SEC16A
protein N-linked glycosylation	32.04	SLC39A8,TMEM165,PGM3,MOGS
transition metal ion transport	29.40	SLC39A8,ATP2C1,TMEM165,SLC39A6,SLC39A14
COPII-coated ER to Golgi transport vesicle	24.84	SEC31A,SCAP,USO1,SEC16A

Table of Contents

CoCl2 18μM R07 exp346

Mechanism of Action

Technical Notes

Protein References

Protein Supplier

Protein Characterization

Dose Response Curve

Screen Summary

Screen Results