Nocodazole 0.1μM R03 exp94

Nocodazole 0.1μM R03 exp94

Mechanism of Action

Inhibits microtubule polymerization and mitotic spindle formation, induces spindle assembly checkpoint (SAC)

Class / Subclass 1: Cell Cycle / Microtubule Poison
Class / Subclass 2: Organelle Function / Cytoskeletal Inhibitor

Technical Notes

Protein References

PubChem Name: Nocodazole
Synonyms: Oncodazole; R17934
CAS #: 31430-18-9
PubChem CID: 4122
IUPAC: methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate
INCHI Name: InChI=1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
INCHI Key: KYRVNWMVYQXFEU-UHFFFAOYSA-N
Molecular Weight: 301.32
Canonical SMILES: COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3
Isomeric SMILES: N/A
Molecular Formula: C14H11N3O3S

Protein Supplier

Supplier Name: Sigma-Aldrich
Catalog #: M1404
Lot #: N/A

Protein Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C14H11N3O3S 302.05939; found 302.05966

Dose Response Curve

Platform ID: Nocodazole
Min: -9.8754; Max: 86.3481

IC	Concentration (µM)
IC10	N/A
IC20	0.0313
IC30	0.0403
IC40	0.0496
IC50	0.0601
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 03
Dose: 100nM
Days of incubation: 8
Doublings: -0.3
Numbers of reads: 10482471

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
0/179	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
COPE	-3.10	0.40
SART1	-2.86	0.43
RPL36A-HNRNPH2	-2.78	0.45
NPM3	-2.72	0.30
ATP6V0B	-2.72	0.48
NAPA	-2.56	0.35
NOM1	-2.46	0.35
MUC15	-2.41	0.35
VPS25	-2.35	0.42
MDH2	-2.34	0.35
ATP12A	-2.32	0.35
BICD2	-2.28	0.35
SLC30A1	-2.24	0.35
CDC73	-2.20	0.42
ZNF830	-2.20	0.35
BOD1L1	-2.18	0.35
SPI1	-2.14	0.38
FBXW9	-2.14	0.35
HCFC1	-2.13	0.68
GPX4	-2.10	0.35
CLEC4C	-2.09	0.35
RFC1	-2.09	0.50
CENPL	-2.08	0.38
CD5L	-2.07	0.35
SCML1	-2.06	0.35
IQCF3	-2.05	0.35
SYVN1	-2.04	0.45
SENP1	-2.02	0.39
DNAJB8	-2.00	0.39
KDM6A	-1.99	0.38
TWIST1	3.58	<0.01
DFNA5	3.59	<0.01
CAMK2B	3.61	<0.01
LRRC72	3.69	<0.01
ACTB	3.75	<0.01
LFNG	3.77	<0.01
PLEKHA8	3.79	<0.01
MYL7	3.82	<0.01
NPY	3.87	<0.01
IQCE	3.98	<0.01
ICA1	3.99	<0.01
CPVL	4.04	<0.01
TTYH3	4.11	<0.01
OSBPL3	4.12	<0.01
ITGB8	4.14	<0.01
CYTH3	4.30	<0.01
ZWILCH	4.31	<0.01
FNTB	4.38	<0.01
SGOL1	4.52	<0.01
AMZ1	4.53	<0.01
RBM14-RBM4	5.25	<0.01
MAD2L1BP	5.51	<0.01
RBM14	5.54	<0.01
BUB1B	5.70	<0.01
MAD2L1	5.89	<0.01
BUB3	6.01	<0.01
MAD1L1	6.44	<0.01
ZW10	6.67	<0.01
KNTC1	7.45	<0.01
TRIP13	8.13	<0.01

Screen	Correlation	Plot
Rotenone 2μM R00 exp33	0.343	View
SB743921 0.001μM R05 exp244	0.341	View
Nocodazole 0.2μM R00 exp24	0.311	View
R-DABN 8μM R03 exp111	0.286	View
Vinblastine 0.015μM R05 exp208	0.225	View
Nocodazole 0.2μM R02 exp64	0.217	View
Vinblastine 0.2μM R03 exp108	0.056	View
DABN racemic mixture R03 exp93	0.338	View
HMS-I1 10μM R06 exp256	0.271	View
BI-6727 0.04μM R03 exp98	0.118	View
BI-2536 0.02μM R03 exp96	0.064	View
GSK461364A 0.1μM R03 exp126	0.06	View

GO Term	Fold Change	Genes
mitotic spindle checkpoint signaling	157.90	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH
spindle assembly checkpoint signaling	157.90	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH
mitotic spindle assembly checkpoint signaling	157.90	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH
negative regulation of mitotic sister chromatid segregation	142.62	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH
negative regulation of mitotic sister chromatid separation	142.62	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH
negative regulation of chromosome segregation	133.98	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH
negative regulation of metaphase/anaphase transition of cell cycle	133.98	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH
negative regulation of chromosome separation	133.98	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH
spindle checkpoint signaling	133.40	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,BUB1B,ZWILCH
negative regulation of mitotic metaphase/anaphase transition	124.80	TRIP13,KNTC1,ZW10,MAD1L1,MAD2L1,BUB1B,ZWILCH
negative regulation of sister chromatid segregation	124.80	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,BUB1B,ZWILCH
negative regulation of nuclear division	96.12	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,ZWILCH
regulation of mitotic sister chromatid segregation	93.85	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH
regulation of mitotic sister chromatid separation	76.23	TRIP13,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH
regulation of chromosome separation	68.14	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH
regulation of mitotic metaphase/anaphase transition	62.10	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH,ACTB
regulation of metaphase/anaphase transition of cell cycle	60.07	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH,ACTB
regulation of sister chromatid segregation	53.66	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH,ACTB
regulation of chromosome segregation	43.86	TRIP13,KNTC1,ZW10,MAD1L1,BUB3,MAD2L1,BUB1B,MAD2L1BP,ZWILCH,ACTB

Table of Contents

Nocodazole 0.1μM R03 exp94

Mechanism of Action

Technical Notes

Protein References

Protein Supplier

Protein Characterization

Dose Response Curve

Screen Summary

Screen Results