Cycloheximide 0.2μM R00 exp15 [The Human Chemical-Genetic Interaction Map Project]

Translation elongation inhibitor, binds 60S ribosomal subunit

Class / Subclass 1: Proteostasis / Translation Inhibitor
Class / Subclass 2: Environmental Stresses / Toxin

Compound References

PubChem Name: Cycloheximide
Synonyms: Naramycin A; Actidione; CHX
CAS #: 66-81-9
PubChem CID: 6197
IUPAC: 4-[(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl]piperidine-2,6-dione
INCHI Name: InChI=1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
INCHI Key: YPHMISFOHDHNIV-FSZOTQKASA-N
Molecular Weight: 281.35
Canonical SMILES: CC1CC(C(=O)C(C1)C(CC2CC(=O)NC(=O)C2)O)C
Isomeric SMILES: C[C@H]1C[C@@H](C(=O)[C@@H](C1)[C@@H](CC2CC(=O)NC(=O)C2)O)C
Molecular Formula: C15H23NO4

Compound Supplier

Supplier Name: Sigma-Aldrich
Catalog #: C7698
Lot #: MKBR7146V

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C15H23NO4 282.16998; found 282.17004

Dose Response Curve

Platform ID: Cycloheximide
Min: 3.7773; Max: 80.9065

IC	Concentration (µM)
IC10	N/A
IC20	0.1270
IC30	0.3675
IC40	0.8782
IC50	1.9535
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 00
Dose: 200nM
Days of incubation: 8
Doublings: 5.6
Numbers of reads: 9929747

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
3/0	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
SETD6	-4.60	<0.01
PRAMEF25	-3.41	0.27
PRAMEF26	-3.39	0.27
DDX39A	-3.09	<0.01
CD2BP2	-2.76	0.02
LAMTOR4	-2.58	0.10
DDX6	-2.53	0.07
AHCY	-2.50	0.07
ORC3	-2.38	0.21
CALML3	-2.37	0.18
DYSF	-2.32	0.15
SCO2	-2.28	0.15
DRG2	-2.28	0.15
PTRH2	-2.27	0.21
KRTAP10-3	-2.23	0.21
CDK2	-2.18	0.26
KRT71	-2.15	0.21
ODC1	-2.15	0.21
GGPS1	-2.12	0.30
FBXO11	-2.12	0.21
TOMM34	-2.12	0.21
ASB3	-2.11	0.21
MEF2A	-2.08	0.35
CFAP45	-2.08	0.67
NEMF	-2.07	0.35
TP53I13	-2.03	0.38
ATF7IP	-2.02	0.28
DCAF15	-2.00	0.43
PRDM10	-1.99	0.44
ZNF598	-1.95	0.46
TNFRSF10D	2.15	0.27
PSMD1	2.15	0.55
ZNF28	2.16	0.27
NSL1	2.16	0.27
GPR183	2.16	0.27
TIMM23B	2.16	0.44
MPC2	2.17	0.27
COPG1	2.19	0.43
NUP153	2.20	0.30
RRP1	2.21	0.27
CCDC90B	2.21	0.27
KRAS	2.22	0.27
VASN	2.22	0.27
DNM2	2.24	0.49
ELOVL5	2.24	0.27
RPL8	2.25	0.57
TMEM165	2.25	0.27
WDR43	2.27	0.40
USP27X	2.30	0.57
MARVELD1	2.30	0.57
PSMD7	2.32	0.40
DDI2	2.45	0.25
BCL2L1	2.52	0.27
RPS6	2.57	0.40
PLK4	2.64	0.29
DTL	2.68	0.27
SCAF1	2.79	0.43
COL28A1	3.00	0.17
KIF11	3.05	0.36
CKAP5	3.28	0.27

Screen	Correlation	Plot
Rifampicin 1μM R00 exp31	0.071	View
Geldanamycin 0.01μM R05 exp235	0.065	View
Rifampicin 10μM R00 exp32	0.061	View
Rapamycin 1μM R00 exp29	0.058	View
Wortmannin 5μM R00 exp38	0.056	View
CCCP 0.1μM R00 exp10	0.074	View
5-Fluorouracil 2μM R00 exp1	0.059	View
Alpha-Amanitin 0.5μM R00 exp5	0.051	View

Last modified: 2026/01/15 21:36
by 127.0.0.1