Table of Contents

UM0131023 0.05μM R05 exp248

UM0131023 0.05μM R05 exp248

Mechanism of Action

Unknown

Class / Subclass 1: Uncharacterized Mechanism / Chemical Screen Hit

Technical Notes

Protein References

PubChem Name: N/A
Synonyms: N/A
CAS #: N/A
PubChem CID: N/A
IUPAC: N/A
INCHI Name: N/A
INCHI Key: N/A
Molecular Weight: 582.61
Canonical SMILES: N/A
Isomeric SMILES: N/A
Molecular Formula: C33H29N2O.C2O2F3

Protein Supplier

Supplier Name: In house - University of Montreal - Chemistry Platform
Catalog #: N/A
Lot #: IRIC-009 / JFL-2177-097 / Batch #01

Protein Characterization

HRMS (ESI-TOF) m/z: (M+NH4)+ Calcd for C33H29N2O 487.26181; found 487.26162

Dose Response Curve

Platform ID: UM0131023
Min: 28.5214; Max: 99.4598

IC	Concentration (µM)
IC10	N/A
IC20	N/A
IC30	0.0253
IC40	0.0424
IC50	0.0681
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 05
Dose: 50nM
Days of incubation: 8
Doublings: 5.8
Numbers of reads: 19294630

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
9/17	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
ABCC1	-3.84	<0.01
GOT1	-3.41	<0.01
GCLM	-3.25	<0.01
SZT2	-3.23	<0.01
GCLC	-2.72	0.04
OSGIN2	-2.69	0.04
C6orf136	-2.67	0.04
SLC25A20	-2.61	0.04
SLC22A14	-2.55	0.04
PTEN	-2.43	0.06
UBE2N	-2.41	0.11
NUDT5	-2.41	0.11
SEPHS2	-2.41	0.06
RNPS1	-2.35	0.21
SRP14	-2.31	0.10
SUCLA2	-2.29	0.18
QRICH1	-2.25	0.26
SUCLG1	-2.25	0.19
UHRF1	-2.22	0.21
METTL16	-2.21	0.55
SWI5	-2.15	0.25
PRDX1	-2.15	0.18
RHOA	-2.12	0.19
SAFB	-2.11	0.26
XRN1	-2.11	0.31
FNTA	-2.08	0.21
ATP8B2	-2.06	0.41
ABHD3	-2.05	0.28
PTPN6	-2.05	0.24
VPS36	-2.03	0.25
MMD	2.31	0.05
MTHFD2	2.34	0.05
RPL14	2.38	0.18
SF3A1	2.40	0.23
SHOC2	2.43	0.06
FAF2	2.45	0.05
TBRG4	2.45	0.05
COPB2	2.47	0.22
C19orf43	2.49	0.05
SLC7A1	2.50	0.15
SHMT2	2.51	0.08
TADA2A	2.51	0.02
HMHA1	2.55	0.04
ERGIC1	2.56	0.04
PRPS1	2.58	0.02
DNAJC24	2.65	0.01
PSME3	2.73	<0.01
POLE2	2.78	0.21
SLC1A5	2.84	0.05
CD19	2.85	0.01
AK6	2.93	<0.01
PDHA1	3.02	<0.01
MDH1	3.04	<0.01
CNOT2	3.05	<0.01
ACSL4	3.11	<0.01
PPRC1	3.14	<0.01
SLC25A32	3.14	<0.01
KCNJ2	3.29	<0.01
ARID4B	3.42	<0.01
MTA2	3.45	<0.01

Screen	Correlation	Plot
Citral 50μM R06 exp274	0.112	View
Deguelin 0.15μM R05 exp209	0.084	View
Phenformin 20μM R05 exp212	0.072	View
Doxycycline 10μM R00 exp18	0.066	View
QNZ 0.01μM R05 exp241	0.062	View
GSK-J4 1-1.25μM to day4 R07 exp363	0.06	View
Hypoxia 5%O2 R04 exp191	0.057	View
Nifuroxazide 5μM R03 exp102	0.057	View
GSK-J4 1μM R07 exp362	0.055	View
Amiloride 100μM R08 exp447	0.052	View
Menadione 5μM R06 exp292	0.051	View

GO Term	Fold Change	Genes
succinate-CoA ligase activity	591.79	SUCLA2,SUCLG1
response to human chorionic gonadotropin	295.89	GCLM,GCLC
response to L-phenylalanine derivative	236.71	GCLM,GCLC
glutamate metabolic process	63.41	GOT1,GCLM,GCLC
dicarboxylic acid metabolic process	26.90	GOT1,GCLM,GCLC,SUCLA2
cellular nitrogen compound catabolic process	12.67	ABCC1,NUDT5,RNPS1,SUCLA2,SUCLG1,METTL16,XRN1
nucleobase-containing compound catabolic process	12.59	NUDT5,RNPS1,SUCLA2,SUCLG1,METTL16,XRN1
heterocycle catabolic process	12.59	ABCC1,NUDT5,RNPS1,SUCLA2,SUCLG1,METTL16,XRN1
aromatic compound catabolic process	12.22	ABCC1,NUDT5,RNPS1,SUCLA2,SUCLG1,METTL16,XRN1
organic cyclic compound catabolic process	11.11	ABCC1,NUDT5,RNPS1,SUCLA2,SUCLG1,METTL16,XRN1