Table of Contents

Alpha-Amanitin 0.5μM R00 exp5

Mechanism of Action

Inhibits RNA polymerase II and III, interferes with translocation

Technical Notes

Protein References

  • PubChem Name: alpha-Amanitin
  • Synonyms: α-Amanitin; α-Amatoxin
  • CAS #: 23109-05-9
  • PubChem CID: 441541
  • IUPAC: 2-[(1R,4S,8R,10S,13S,16S,34S)-34-[(2S)-butan-2-yl]-13-[(2R,3R)-3,4-dihydroxybutan-2-yl]-8,22-dihydroxy-2,5,11,14,27,30,33,36,39-nonaoxo-27Œª4-thia-3,6,12,15,25,29,32,35,38-nonazapentacyclo[14.12.11.06,10.018,26.019,24]nonatriaconta-18(26),19(24),20,22-tetraen-4-yl]acetamide
  • INCHI Name: InChI=1S/C39H54N10O14S/c1-4-16(2)31-36(60)42-11-29(55)43-25-15-64(63)38-21(20-6-5-18(51)7-22(20)46-38)9-23(33(57)41-12-30(56)47-31)44-37(61)32(17(3)27(53)14-50)48-35(59)26-8-19(52)13-49(26)39(62)24(10-28(40)54)45-34(25)58/h5-7,16-17,19,23-27,31-32,46,50-53H,4,8-15H2,1-3H3,(H2,40,54)(H,41,57)(H,42,60)(H,43,55)(H,44,61)(H,45,58)(H,47,56)(H,48,59)/t16-,17-,19+,23-,24-,25-,26-,27-,31-,32-,64?/m0/s1
  • INCHI Key: CIORWBWIBBPXCG-JAXJKTSHSA-N
  • Molecular Weight: 919
  • Canonical SMILES: CCC(C)C1C(=O)NCC(=O)NC2CS(=O)C3=C(CC(C(=O)NCC(=O)N1)NC(=O)C(NC(=O)C4CC(CN4C(=O)C(NC2=O)CC(=O)N)O)C(C)C(CO)O)C5=C(N3)C=C(C=C5)O
  • Isomeric SMILES: CC[C@H](C)[C@H]1C(=O)NCC(=O)N[C@H]2CS(=O)C3=C(C[C@@H](C(=O)NCC(=O)N1)NC(=O)[C@@H](NC(=O)[C@@H]4C[C@H](CN4C(=O)[C@@H](NC2=O)CC(=O)N)O)[C@@H](C)[C@H](CO)O)C5=C(N3)C=C(C=C5)O
  • Molecular Formula: C39H54N10O14S

Protein Supplier

  • Supplier Name: Sigma-Aldrich
  • Catalog #: A2263
  • Lot #: N/A

Protein Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C39H54N10O14S 919.36144; found 919.36336

Dose Response Curve

  • Platform ID: Alpha-amanitin
  • Min: -15.5942; Max: 56.4508





IC Concentration (µM)
IC10 N/A
IC20 9.6912
IC30 15.9233
IC40 23.9227
IC50 34.7566
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
7/0ScoresViewViewViewViewView