Rapamycin 1μM R00 exp29

Allosteric mTORC1 inhibitor, forms ternary complex with FKBP12 and mTOR, attenuates protein synthesis and metabolism

PubChem Name: Sirolimus
Synonyms: Sirolimus; AY 22989
CAS #: 53123-88-9
PubChem CID: 5284616
IUPAC: (1R,9S,12S,15R,16E,18R,19R,21R,23S,24E,26E,28E,30S,32S,35R)-1,18-dihydroxy-12-[(2R)-1-[(1S,3R,4R)-4-hydroxy-3-methoxycyclohexyl]propan-2-yl]-19,30-dimethoxy-15,17,21,23,29,35-hexamethyl-11,36-dioxa-4-azatricyclo[30.3.1.04,9]hexatriaconta-16,24,26,28-tetraene-2,3,10,14,20-pentone
INCHI Name: InChI=1S/C51H79NO13/c1-30-16-12-11-13-17-31(2)42(61-8)28-38-21-19-36(7)51(60,65-38)48(57)49(58)52-23-15-14-18-39(52)50(59)64-43(33(4)26-37-20-22-40(53)44(27-37)62-9)29-41(54)32(3)25-35(6)46(56)47(63-10)45(55)34(5)24-30/h11-13,16-17,25,30,32-34,36-40,42-44,46-47,53,56,60H,14-15,18-24,26-29H2,1-10H3/b13-11+,16-12+,31-17+,35-25+/t30-,32-,33-,34-,36-,37+,38+,39+,40-,42+,43+,44-,46-,47+,51-/m1/s1
INCHI Key: QFJCIRLUMZQUOT-HPLJOQBZSA-N
Molecular Weight: 914.2
Canonical SMILES: CC1CCC2CC(C(=CC=CC=CC(CC(C(=O)C(C(C(=CC(C(=O)CC(OC(=O)C3CCCCN3C(=O)C(=O)C1(O2)O)C(C)CC4CCC(C(C4)OC)O)C)C)O)OC)C)C)C)OC
Isomeric SMILES: C[C@@H]1CC[C@H]2C[C@@H](/C(=C/C=C/C=C/[C@H](C[C@H](C(=O)[C@@H]([C@@H](/C(=C/[C@H](C(=O)C[C@H](OC(=O)[C@@H]3CCCCN3C(=O)C(=O)[C@@]1(O2)O)[C@H](C)C[C@@H]4CC[C@H]([C@@H](C4)OC)O)C)/C)O)OC)C)C)/C)OC
Molecular Formula: C51H79NO13

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
7/45	Scores	View	View	View	View	View

Screen	Correlation	Plot
Rapamycin 2μM R07 exp306	0.14	View
Rapamycin 2μM plus FK-506 5μM R07 exp308	0.102	View
Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307	0.079	View
Torin1 0.08μM R02 exp82	0.055	View
Wortmannin 0.5μM R00 exp37	0.051	View
Rapamycin 10μM R00 exp30	0.185	View
Rapamycin 0.001μM R08 exp429	0.164	View
Dexamethasone 0.006 to 0.015μM on day4 R07 exp352	0.06	View
Dexamethasone 0.006μM R07 exp351	0.059	View
Cycloheximide 0.2μM R00 exp15	0.058	View
INK128 0.2μM R02 exp70	0.057	View
KU-0063794 3.8μM R02 exp71	0.054	View
Oligomycin-A 2μM R00 exp25	0.054	View
MK2206 4μM R08 exp504	0.052	View
DABN 2μM R00 exp16	0.052	View
Chloramphenicol 20μM R00 exp13	0.052	View

GO Term	Fold Change	Genes
negative regulation of calcium ion import across plasma membrane	276.35	PPP3R1,PPP3CA
GID complex	276.35	WDR26,MAEA
CCR4-NOT core complex	221.08	CNOT2,CNOT7
cellular response to interleukin-3	221.08	GSK3B,GSK3A
negative regulation of glycogen biosynthetic process	221.08	GSK3B,GSK3A
poly(A)-specific ribonuclease activity	138.18	CNOT8,CNOT2,CNOT7
3-5-RNA exonuclease activity	47.37	CNOT8,CNOT2,CNOT7
RNA exonuclease activity, producing 5-phosphomonoesters	43.63	CNOT8,CNOT2,CNOT7
regulation of calcineurin-NFAT signaling cascade	43.63	PPP3R1,PPP3CA,GSK3B
regulation of calcineurin-mediated signaling	42.52	PPP3R1,PPP3CA,GSK3B
proteasome-mediated ubiquitin-dependent protein catabolic process	11.25	UBE2H,WDR26,MAEA,GSK3B,PSMD11,GID8,GSK3A
proteasomal protein catabolic process	10.21	UBE2H,WDR26,MAEA,GSK3B,PSMD11,GID8,GSK3A
proteolysis involved in protein catabolic process	7.60	UBE2H,WDR26,MAEA,GSK3B,PSMD11,CLPP,GID8,GSK3A
ubiquitin-dependent protein catabolic process	7.59	UBE2H,WDR26,MAEA,GSK3B,PSMD11,GID8,GSK3A
intracellular protein-containing complex	7.57	YPEL5,CNOT8,PPP3R1,CNOT2,PPP3CA,WDR26,MAEA,GSK3B,PSMD11,CNOT7,GID8,GSK3A
modification-dependent protein catabolic process	7.44	UBE2H,WDR26,MAEA,GSK3B,PSMD11,GID8,GSK3A
modification-dependent macromolecule catabolic process	7.29	UBE2H,WDR26,MAEA,GSK3B,PSMD11,GID8,GSK3A
macromolecule catabolic process	7.20	CNOT8,UBE2H,CNOT2,WDR26,MAEA,GSK3B,PSMD11,CNOT7,BTK,CLPP,GID8,GSK3A
protein catabolic process	7.17	UBE2H,WDR26,MAEA,GSK3B,PSMD11,BTK,CLPP,GID8,GSK3A

GO Term	Fold Change	Genes
GTPase activator complex	177.65	LAMTOR4,RRAGA,LAMTOR2
enzyme activator complex	118.44	LAMTOR4,RRAGA,LAMTOR2