EIF3A

Gene Information

Official Symbol: EIF3A
Official Name: eukaryotic translation initiation factor 3 subunit A
Aliases and Previous Symbols: N/A
Entrez ID: 8661
UniProt: Q14152
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF- 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815). {ECO:0000255|HAMAP- Rule:MF_03000, ECO:0000269|PubMed:11169732, ECO:0000269|PubMed:17581632, ECO:0000269|PubMed:25849773, ECO:0000269|PubMed:27462815}. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426). {ECO:0000269|PubMed:18056426}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
CR131-b 0.005μM R08 exp474	-1.91
Dorsomorphin 5μM R07 exp357	1.7
LB-100 2μM R05 exp210	1.7
BAY-598 4μM R05 exp220	1.71
Oligomycin-A 2μM R00 exp25	1.72
Docetaxel 0.001 to 0.002μM on day4 R04 exp159	1.74
Ixabepilone 0.004 to 0.005μM on day4 R04 exp184	1.75
Chlorpromazine 10μM R07 exp344	1.76
Pomalidomide 20μM R07 exp391	1.77
All-trans-Retinoic-Acid 40μM R07 exp334	1.79
Deguelin 0.05μM R02 exp69	1.8
Nifuroxazide 1μM R01 exp51	1.8
MLN-4924 0.2μM R00 exp21	1.82
CVT-10216 0.1μM R06 exp278	1.83
Tozasertib 0.1μM R07 exp414	1.83
Salubrinal 20μM R07 exp399	1.84
THZ531 0.225μM R08 exp444	1.85
Caffeine 2600μM R08 exp463	1.87
FK-506 5μM R07 exp358	1.87
Hypoxia 5%O2 R04 exp191	1.91
CB-839 10μM R05 exp224	1.91
Demecolcine 0.03μM R05 exp228	1.95
NMS-873 0.07μM R07 exp380	1.97
BI-6727 0.0125μM R03 exp97	1.98
Trientine 500μM R08 exp534	1.99
Pimelic-diphenylamide-106 0.5μM R00 exp27	2.03
Betulinic acid 10 to 15μM on day4 R05 exp222	2.04
Mubritinib 0.2μM R02 exp65	2.04
Docetaxel 0.002μM R01 exp45	2.08
Ibrutinib 10μM R08 exp442	2.08
AICAR 240μM R05 exp211	2.11
Lead acetate 2000μM R08 exp498 no dilution day6	2.11
Vemurafenib 6.6μM R08 exp434	2.16
2-Deoxy-D-glucose 800μM R05 exp214	2.17
Wortmannin 5μM R00 exp38	2.17
Azithromycin 100μM R08 exp452	2.18
TRAIL 5ng/ml R00 exp35	2.22
NN-Diethyl-meta-toluamide 500μM R08 exp438	2.29
Latrunculin-B 10μM R07 exp374	2.3
TRAIL 50ng/ml R00 exp36	2.32
Citral 75μM R06 exp275	2.37
Dexamethasone 0.006 to 0.015μM on day4 R07 exp352	2.37
Lapatinib 5μM R01 exp47	2.37
LB-100 4.1μM R02 exp72	2.41
Pifithrin-alpha 20μM R07 exp390	2.45
NN-Dimethylsphingosine 2.5μM R08 exp508	2.47
Nicotinamide 2000μM R01 exp50	2.47
Dexamethasone 0.006μM R07 exp351	2.52
Tubacin 1.6μM R07 exp416	2.6
Colchicine 0.009μM R05 exp215	2.62
BI-2536 0.0042μM R03 exp95	2.67
Lead acetate 2000μM R08 exp497	2.69
Radicicol 0.16μM R05 exp242	2.74
Erlotinib 10μM R05 exp216	2.8
Pyridostatin 4μM R05 exp240	3.23
Mubritinib 0.2μM R01 exp48	3.73

Gene	Correlation
EIF3G	0.581
HGC6.3	0.484
RPS12	0.433
TFIP11	0.422
PSMB4	0.421
EIF3CL	0.419
EIF4B	0.417
GOLGA6L1	0.411
TP53TG3B	0.401
TP53TG3	0.4
TP53TG3C	0.4

Global Fraction of Cell Lines Where Essential: 739/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	26/26
breast	33/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	21/21
liver	20/20
lung	75/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 1483
Expression level (log2 read counts): 9.58

Expression Distribution

eukaryotic translation initiation factor 3 complex, eIF3e
multi-eIF complex
translation reinitiation
viral translational termination-reinitiation
eukaryotic translation initiation factor 3 complex, eIF3m
IRES-dependent viral translational initiation
viral translation
eukaryotic 48S preinitiation complex
eukaryotic translation initiation factor 3 complex
formation of cytoplasmic translation initiation complex
eukaryotic 43S preinitiation complex
cytoplasmic translational initiation
translation initiation factor activity
receptor tyrosine kinase binding
negative regulation of ERK1 and ERK2 cascade
cytoplasmic translation
viral gene expression
translational initiation
mRNA binding
negative regulation of MAPK cascade
structural molecule activity
ribonucleoprotein complex assembly
ribonucleoprotein complex subunit organization
postsynaptic density
regulation of ERK1 and ERK2 cascade
microtubule
translation
negative regulation of protein phosphorylation
peptide biosynthetic process
negative regulation of phosphorylation
ribonucleoprotein complex biogenesis
negative regulation of intracellular signal transduction
amide biosynthetic process
peptide metabolic process
negative regulation of phosphate metabolic process
negative regulation of phosphorus metabolic process
negative regulation of protein modification process
viral process
regulation of MAPK cascade
symbiotic process