GSK461364A 0.1μM R03 exp126

Inhibits PLK1

PubChem Name: Gsk-461364
Synonyms: GSK461364A
CAS #: 929095-18-1
PubChem CID: 15983966
IUPAC: 5-[6-[(4-methylpiperazin-1-yl)methyl]benzimidazol-1-yl]-3-[(1R)-1-[2-(trifluoromethyl)phenyl]ethoxy]thiophene-2-carboxamide
INCHI Name: InChI=1S/C27H28F3N5O2S/c1-17(19-5-3-4-6-20(19)27(28,29)30)37-23-14-24(38-25(23)26(31)36)35-16-32-21-8-7-18(13-22(21)35)15-34-11-9-33(2)10-12-34/h3-8,13-14,16-17H,9-12,15H2,1-2H3,(H2,31,36)/t17-/m1/s1
INCHI Key: ZHJGWYRLJUCMRT-QGZVFWFLSA-N
Molecular Weight: 543.6
Canonical SMILES: CC(C1=CC=CC=C1C(F)(F)F)OC2=C(SC(=C2)N3C=NC4=C3C=C(C=C4)CN5CCN(CC5)C)C(=O)N
Isomeric SMILES: C[C@H](C1=CC=CC=C1C(F)(F)F)OC2=C(SC(=C2)N3C=NC4=C3C=C(C=C4)CN5CCN(CC5)C)C(=O)N
Molecular Formula: C27H28F3N5O2S

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C27H28F3N5O2S 544.19886; found 544.20114

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
1/18	Scores	View	View	View	View	View

Screen	Correlation	Plot
Vinblastine 0.2μM R03 exp108	0.141	View
MLN-4924 2μM R00 exp22	0.096	View
SNS-032 15μM R08 exp443	0.081	View
Hinokiflavone 12μM R08 exp487	0.074	View
PU-H71 1μM R08 exp515	0.07	View
R-DABN 8μM R03 exp111	0.064	View
Pimelic-diphenylamide-106 5μM R00 exp28	0.062	View
THZ1 0.06μM R08 exp531	0.062	View
MS023 7μM R03 exp135	0.06	View
Nocodazole 0.1μM R03 exp94	0.06	View
Mitomycin-C 0.06μM R08 exp503	0.057	View
Nocodazole 0.2μM R02 exp64	0.052	View
UM131593 0.2μM R03 exp106	0.052	View
BI-2536 0.02μM R03 exp96	0.175	View
BI-6727 0.04μM R03 exp98	0.157	View
42°C R08 exp539	0.09	View
Bleomycin 5μM R08 exp459	0.087	View
DABN racemic mixture R03 exp93	0.085	View
Ammonium tetrathiomolybdate 10μM R08 exp448	0.078	View
CI-1040 9.5μM R08 exp472	0.073	View
Atovaquone 15μM R08 exp451	0.07	View
5-Fluorouracil 20μM R00 exp2	0.068	View
Benzoate 20000μM R08 exp456	0.065	View
Aphidicolin 0.4μM R08 exp440	0.064	View
Actinomycin-D 0.001μM R00 exp3	0.064	View
Doxorubicin 0.02μM R08 exp478	0.062	View
Etoposide 1μM R00 exp19	0.062	View
DABN 20μM R00 exp17	0.057	View
Bortezomib 0.05μM R00 exp7	0.055	View
Etoposide 0.3μM R05 exp199	0.052	View

GO Term	Fold Change	Genes
regulation of anaphase-promoting complex-dependent catabolic process	296.07	CDC20,PLK1
female meiosis chromosome segregation	296.07	TTK,PLK1
protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	126.89	VPS25,VPS37B,VPS28
mitotic spindle assembly checkpoint signaling	63.44	CDC20,TTK,PLK1
spindle checkpoint signaling	61.26	CDC20,TTK,PLK1
multivesicular body assembly	59.21	VPS25,VPS37B,VPS28
negative regulation of sister chromatid segregation	57.30	CDC20,TTK,PLK1
multivesicular body organization	57.30	VPS25,VPS37B,VPS28
negative regulation of mitotic sister chromatid separation	57.30	CDC20,TTK,PLK1
negative regulation of mitotic metaphase/anaphase transition	57.30	CDC20,TTK,PLK1
negative regulation of chromosome separation	53.83	CDC20,TTK,PLK1
negative regulation of chromosome segregation	53.83	CDC20,TTK,PLK1
late endosome to vacuole transport	52.25	VPS25,VPS37B,VPS28
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	50.76	VPS25,VPS37B,VPS28
endosome transport via multivesicular body sorting pathway	45.55	VPS25,VPS37B,VPS28
negative regulation of mitotic nuclear division	45.55	CDC20,TTK,PLK1

GO Term	Fold Change	Genes
GDP-mannose biosynthetic process	368.44	GMPPB,PMM2
proton-transporting ATP synthase complex, catalytic core F(1)	221.07	ATP5F1B,ATP5PO
nucleotide-sugar biosynthetic process	97.53	GMPPB,GFPT1,PMM2
nucleotide-sugar metabolic process	51.81	DPAGT1,GMPPB,PMM2
transcription by RNA polymerase III	43.63	GTF3A,GTF3C4,POLR3H
protein N-linked glycosylation	32.04	DPAGT1,GFPT1,ALG2,PMM2
DNA-templated transcription initiation	21.67	GTF2A2,GTF3C4,MED16,POLR3H
tRNA metabolic process	17.64	TRMT10A,THG1L,RPP21,RTCB,GTF3C4,YARS1
protein glycosylation	14.94	DPAGT1,GMPPB,GFPT1,ALG2,PMM2
macromolecule glycosylation	14.94	DPAGT1,GMPPB,GFPT1,ALG2,PMM2
glycosylation	13.29	DPAGT1,GMPPB,GFPT1,ALG2,PMM2
ncRNA metabolic process	8.74	TRMT10A,THG1L,PELO,RPP21,RTCB,GTF3A,GTF3C4,YARS1
nucleobase-containing compound biosynthetic process	7.31	GMPPB,ATP5F1B,GTF2A2,ATP5PO,GTF3A,GTF3C4,GFPT1,MED16,ENY2,POLR3H,PMM2
carbohydrate derivative biosynthetic process	6.97	DPAGT1,GMPPB,ATP5F1B,ATP5PO,GFPT1,ALG2,PMM2
heterocycle biosynthetic process	6.70	GMPPB,ATP5F1B,GTF2A2,ATP5PO,GTF3A,GTF3C4,GFPT1,MED16,ENY2,POLR3H,PMM2
aromatic compound biosynthetic process	6.64	GMPPB,ATP5F1B,GTF2A2,ATP5PO,GTF3A,GTF3C4,GFPT1,MED16,ENY2,POLR3H,PMM2